Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3529 and GFF3530 are separated by 91 nucleotides GFF3530 and GFF3531 are separated by 11 nucleotides GFF3531 and GFF3532 are separated by 76 nucleotides GFF3532 and GFF3533 are separated by 313 nucleotides
GFF3529 - Vitamin B12 ABC transporter, permease component BtuC, at 281,624 to 282,604
GFF3529
GFF3530 - Glutathione peroxidase family protein, at 282,696 to 283,247
GFF3530
GFF3531 - Vitamin B12 ABC transporter, ATPase component BtuD, at 283,259 to 283,996
GFF3531
GFF3532 - Probable lipoprotein nlpC precursor, at 284,073 to 284,537
GFF3532
GFF3533 - FIG00638687: hypothetical protein, at 284,851 to 285,564
GFF3533
Position (kb) 283
284 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 282.421 kb on - strand, within GFF3529 at 282.487 kb on - strand, within GFF3529 at 282.642 kb on - strand at 282.648 kb on - strand at 282.750 kb on - strand at 283.245 kb on + strand at 283.371 kb on + strand, within GFF3531 at 283.371 kb on + strand, within GFF3531 at 283.382 kb on - strand, within GFF3531 at 283.668 kb on + strand, within GFF3531 at 283.773 kb on + strand, within GFF3531 at 283.774 kb on - strand, within GFF3531 at 283.782 kb on + strand, within GFF3531 at 283.783 kb on - strand, within GFF3531 at 284.213 kb on + strand, within GFF3532 at 284.614 kb on - strand at 284.618 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 282,421 - GFF3529 0.81 -1.1 282,487 - GFF3529 0.88 -1.1 282,642 - -0.2 282,648 - -1.0 282,750 - -3.2 283,245 + -1.0 283,371 + GFF3531 0.15 +1.6 283,371 + GFF3531 0.15 +2.0 283,382 - GFF3531 0.17 -1.6 283,668 + GFF3531 0.55 +0.4 283,773 + GFF3531 0.70 +2.1 283,774 - GFF3531 0.70 -0.7 283,782 + GFF3531 0.71 +2.4 283,783 - GFF3531 0.71 -1.7 284,213 + GFF3532 0.30 +0.9 284,614 - +0.9 284,618 - -2.0
Or see this region's nucleotide sequence