Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3457 and GFF3458 are separated by 229 nucleotides GFF3458 and GFF3459 are separated by 121 nucleotides GFF3459 and GFF3460 are separated by 7 nucleotides GFF3460 and GFF3461 are separated by 61 nucleotides GFF3461 and GFF3462 are separated by 254 nucleotides
GFF3457 - FIG074102: hypothetical protein, at 213,823 to 214,005
GFF3457
GFF3458 - Putative membrane protein, at 214,235 to 214,876
GFF3458
GFF3459 - FIG01045255: hypothetical protein, at 214,998 to 215,183
GFF3459
GFF3460 - Transglycosylase associated protein, at 215,191 to 215,439
GFF3460
GFF3461 - hypothetical protein, at 215,501 to 215,617
GFF3461
GFF3462 - FIG00638802: hypothetical protein, at 215,872 to 216,123
GFF3462
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1 at 214.108 kb on + strand at 214.113 kb on - strand at 214.228 kb on + strand at 214.270 kb on + strand at 214.270 kb on + strand at 214.409 kb on - strand, within GFF3458 at 214.409 kb on - strand, within GFF3458 at 214.409 kb on - strand, within GFF3458 at 214.411 kb on - strand, within GFF3458 at 214.486 kb on + strand, within GFF3458 at 214.732 kb on + strand, within GFF3458 at 214.780 kb on + strand, within GFF3458 at 215.171 kb on - strand at 215.280 kb on + strand, within GFF3460 at 215.332 kb on - strand, within GFF3460 at 215.332 kb on - strand, within GFF3460 at 215.485 kb on - strand at 215.537 kb on + strand, within GFF3461 at 215.680 kb on - strand at 215.683 kb on - strand at 215.711 kb on - strand at 215.860 kb on + strand at 215.903 kb on + strand, within GFF3462 at 216.150 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 214,108 + +0.2 214,113 - +0.0 214,228 + -1.0 214,270 + +0.1 214,270 + +0.1 214,409 - GFF3458 0.27 -0.0 214,409 - GFF3458 0.27 +0.6 214,409 - GFF3458 0.27 +0.8 214,411 - GFF3458 0.27 +0.4 214,486 + GFF3458 0.39 +0.4 214,732 + GFF3458 0.77 +0.3 214,780 + GFF3458 0.85 -1.7 215,171 - +0.5 215,280 + GFF3460 0.36 -1.6 215,332 - GFF3460 0.57 +1.3 215,332 - GFF3460 0.57 -2.0 215,485 - -0.2 215,537 + GFF3461 0.31 -0.2 215,680 - +0.2 215,683 - +0.3 215,711 - +0.6 215,860 + -0.3 215,903 + GFF3462 0.12 +0.1 216,150 + -0.4
Or see this region's nucleotide sequence