Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3414 and GFF3415 overlap by 1 nucleotides GFF3415 and GFF3416 are separated by 123 nucleotides GFF3416 and GFF3417 are separated by 3 nucleotides GFF3417 and GFF3418 are separated by 86 nucleotides
GFF3414 - Sensor protein PhoQ (EC 2.7.13.3), at 179,278 to 180,741
GFF3414
GFF3415 - Transcriptional regulatory protein PhoP, at 180,741 to 181,415
GFF3415
GFF3416 - Adenylosuccinate lyase (EC 4.3.2.2), at 181,539 to 182,909
GFF3416
GFF3417 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 182,913 to 183,554
GFF3417
GFF3418 - tRNA-specific 2-thiouridylase MnmA, at 183,641 to 184,747
GFF3418
Position (kb) 181
182
183 Strain fitness (log2 ratio) -1
0
1
2
3 at 180.561 kb on + strand, within GFF3414 at 180.736 kb on - strand at 180.849 kb on - strand, within GFF3415 at 180.877 kb on + strand, within GFF3415 at 181.079 kb on + strand, within GFF3415 at 181.257 kb on + strand, within GFF3415 at 181.298 kb on - strand, within GFF3415 at 181.405 kb on - strand at 181.483 kb on + strand at 181.541 kb on - strand at 181.545 kb on - strand at 182.982 kb on - strand, within GFF3417 at 183.143 kb on - strand, within GFF3417 at 183.166 kb on - strand, within GFF3417 at 183.234 kb on - strand, within GFF3417 at 183.291 kb on - strand, within GFF3417 at 183.291 kb on - strand, within GFF3417 at 183.405 kb on - strand, within GFF3417 at 183.572 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 180,561 + GFF3414 0.88 +2.0 180,736 - +1.2 180,849 - GFF3415 0.16 +2.8 180,877 + GFF3415 0.20 +2.5 181,079 + GFF3415 0.50 +1.9 181,257 + GFF3415 0.76 +2.8 181,298 - GFF3415 0.83 +2.0 181,405 - +3.2 181,483 + +1.2 181,541 - +0.4 181,545 - -1.4 182,982 - GFF3417 0.11 +1.8 183,143 - GFF3417 0.36 +1.4 183,166 - GFF3417 0.39 +0.6 183,234 - GFF3417 0.50 +2.1 183,291 - GFF3417 0.59 +2.3 183,291 - GFF3417 0.59 +2.0 183,405 - GFF3417 0.77 +1.6 183,572 - +0.3
Or see this region's nucleotide sequence