Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3373 and GFF3374 are separated by 15 nucleotides GFF3374 and GFF3375 are separated by 12 nucleotides GFF3375 and GFF3376 are separated by 155 nucleotides GFF3376 and GFF3377 are separated by 121 nucleotides
GFF3373 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41), at 139,364 to 140,317
GFF3373
GFF3374 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 140,333 to 141,262
GFF3374
GFF3375 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 141,275 to 142,009
GFF3375
GFF3376 - Acyl carrier protein, at 142,165 to 142,401
GFF3376
GFF3377 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 142,523 to 143,728
GFF3377
Position (kb) 141
142
143 Strain fitness (log2 ratio) -1
0
1
2
3 at 142.033 kb on + strand at 142.033 kb on + strand at 142.456 kb on + strand at 142.492 kb on + strand at 142.549 kb on - strand at 142.644 kb on + strand, within GFF3377 at 142.657 kb on + strand, within GFF3377 at 142.931 kb on - strand, within GFF3377 at 142.931 kb on - strand, within GFF3377 at 142.937 kb on + strand, within GFF3377 at 142.937 kb on + strand, within GFF3377 at 142.938 kb on - strand, within GFF3377
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 142,033 + +2.4 142,033 + +2.2 142,456 + +2.8 142,492 + +3.6 142,549 - +3.6 142,644 + GFF3377 0.10 +1.9 142,657 + GFF3377 0.11 +2.6 142,931 - GFF3377 0.34 +3.1 142,931 - GFF3377 0.34 +2.4 142,937 + GFF3377 0.34 +2.1 142,937 + GFF3377 0.34 +3.3 142,938 - GFF3377 0.34 +1.9
Or see this region's nucleotide sequence