Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3238 and GFF3239 are separated by 98 nucleotides GFF3239 and GFF3240 are separated by 12 nucleotides
GFF3238 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1, at 10,542 to 11,651
GFF3238
GFF3239 - 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-), at 11,750 to 13,858
GFF3239
GFF3240 - COG0488: ATPase components of ABC transporters with duplicated ATPase domains, at 13,871 to 15,778
GFF3240
Position (kb)
11
12
13
14 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 10.780 kb on - strand, within GFF3238 at 10.837 kb on + strand, within GFF3238 at 11.093 kb on + strand, within GFF3238 at 11.094 kb on - strand, within GFF3238 at 11.154 kb on + strand, within GFF3238 at 11.209 kb on - strand, within GFF3238 at 11.249 kb on + strand, within GFF3238 at 11.250 kb on - strand, within GFF3238 at 11.430 kb on - strand, within GFF3238 at 11.573 kb on + strand at 11.627 kb on + strand at 11.712 kb on - strand at 11.878 kb on + strand at 11.918 kb on - strand at 11.966 kb on + strand, within GFF3239 at 12.061 kb on + strand, within GFF3239 at 12.062 kb on - strand, within GFF3239 at 12.268 kb on + strand, within GFF3239 at 12.296 kb on - strand, within GFF3239 at 12.820 kb on - strand, within GFF3239 at 12.820 kb on - strand, within GFF3239 at 12.879 kb on - strand, within GFF3239 at 13.010 kb on + strand, within GFF3239 at 13.133 kb on - strand, within GFF3239 at 13.185 kb on + strand, within GFF3239 at 13.266 kb on - strand, within GFF3239 at 13.268 kb on - strand, within GFF3239 at 13.278 kb on + strand, within GFF3239 at 13.391 kb on + strand, within GFF3239 at 13.454 kb on - strand, within GFF3239 at 13.718 kb on - strand at 13.869 kb on - strand at 14.582 kb on + strand, within GFF3240 at 14.856 kb on + strand, within GFF3240 at 14.857 kb on - strand, within GFF3240
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 10,780 - GFF3238 0.21 +0.7 10,837 + GFF3238 0.27 -1.5 11,093 + GFF3238 0.50 -1.6 11,094 - GFF3238 0.50 +0.1 11,154 + GFF3238 0.55 +0.1 11,209 - GFF3238 0.60 +0.7 11,249 + GFF3238 0.64 +0.5 11,250 - GFF3238 0.64 +0.6 11,430 - GFF3238 0.80 +0.2 11,573 + +2.2 11,627 + +1.1 11,712 - +0.3 11,878 + -2.1 11,918 - -0.0 11,966 + GFF3239 0.10 +0.1 12,061 + GFF3239 0.15 -0.9 12,062 - GFF3239 0.15 +0.2 12,268 + GFF3239 0.25 +0.9 12,296 - GFF3239 0.26 -0.5 12,820 - GFF3239 0.51 +0.0 12,820 - GFF3239 0.51 -0.5 12,879 - GFF3239 0.54 -0.7 13,010 + GFF3239 0.60 +0.2 13,133 - GFF3239 0.66 -0.7 13,185 + GFF3239 0.68 +0.5 13,266 - GFF3239 0.72 -2.3 13,268 - GFF3239 0.72 -0.8 13,278 + GFF3239 0.72 -2.0 13,391 + GFF3239 0.78 -0.1 13,454 - GFF3239 0.81 -2.1 13,718 - +0.4 13,869 - -0.6 14,582 + GFF3240 0.37 -2.2 14,856 + GFF3240 0.52 -0.0 14,857 - GFF3240 0.52 +0.1
Or see this region's nucleotide sequence