Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3233 and GFF3234 are separated by 270 nucleotides GFF3234 and GFF3235 are separated by 426 nucleotides GFF3235 and GFF3236 are separated by 207 nucleotides
GFF3233 - FIG01046696: hypothetical protein, at 4,432 to 5,118
GFF3233
GFF3234 - Virulence protein msgA, at 5,389 to 5,580
GFF3234
GFF3235 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 6,007 to 8,619
GFF3235
GFF3236 - Dihydroorotate dehydrogenase (EC 1.3.3.1), at 8,827 to 9,837
GFF3236
Position (kb) 6
7
8
9 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5.581 kb on - strand at 5.904 kb on + strand at 5.963 kb on + strand at 5.983 kb on - strand at 6.191 kb on + strand at 6.196 kb on - strand at 6.226 kb on + strand at 6.227 kb on - strand at 6.299 kb on + strand, within GFF3235 at 6.417 kb on - strand, within GFF3235 at 6.429 kb on + strand, within GFF3235 at 6.755 kb on - strand, within GFF3235 at 6.755 kb on - strand, within GFF3235 at 6.757 kb on - strand, within GFF3235 at 6.972 kb on - strand, within GFF3235 at 7.059 kb on - strand, within GFF3235 at 7.126 kb on - strand, within GFF3235 at 7.272 kb on - strand, within GFF3235 at 7.293 kb on + strand, within GFF3235 at 7.293 kb on + strand, within GFF3235 at 7.636 kb on + strand, within GFF3235 at 7.638 kb on + strand, within GFF3235 at 7.639 kb on - strand, within GFF3235 at 7.729 kb on + strand, within GFF3235 at 7.730 kb on - strand, within GFF3235 at 7.730 kb on - strand, within GFF3235 at 7.730 kb on - strand, within GFF3235 at 7.734 kb on + strand, within GFF3235 at 7.734 kb on + strand, within GFF3235 at 7.735 kb on - strand, within GFF3235 at 7.811 kb on + strand, within GFF3235 at 7.984 kb on + strand, within GFF3235 at 7.984 kb on + strand, within GFF3235 at 8.094 kb on + strand, within GFF3235 at 8.098 kb on - strand, within GFF3235 at 8.134 kb on - strand, within GFF3235 at 8.217 kb on + strand, within GFF3235 at 8.332 kb on - strand, within GFF3235 at 8.512 kb on + strand at 8.520 kb on + strand at 8.605 kb on - strand at 8.753 kb on - strand at 8.830 kb on + strand at 8.831 kb on - strand at 8.847 kb on + strand at 8.914 kb on - strand at 8.982 kb on + strand, within GFF3236 at 9.048 kb on - strand, within GFF3236 at 9.171 kb on + strand, within GFF3236 at 9.229 kb on - strand, within GFF3236 at 9.328 kb on + strand, within GFF3236 at 9.408 kb on - strand, within GFF3236 at 9.453 kb on + strand, within GFF3236 at 9.454 kb on - strand, within GFF3236 at 9.457 kb on + strand, within GFF3236 at 9.457 kb on + strand, within GFF3236
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 5,581 - +0.5 5,904 + +0.4 5,963 + +0.9 5,983 - +0.2 6,191 + -0.7 6,196 - -1.1 6,226 + -0.6 6,227 - +0.3 6,299 + GFF3235 0.11 +0.2 6,417 - GFF3235 0.16 -0.8 6,429 + GFF3235 0.16 +0.1 6,755 - GFF3235 0.29 +1.2 6,755 - GFF3235 0.29 +0.2 6,757 - GFF3235 0.29 -0.1 6,972 - GFF3235 0.37 +0.6 7,059 - GFF3235 0.40 +0.2 7,126 - GFF3235 0.43 -1.0 7,272 - GFF3235 0.48 +0.3 7,293 + GFF3235 0.49 +0.1 7,293 + GFF3235 0.49 -0.8 7,636 + GFF3235 0.62 -0.1 7,638 + GFF3235 0.62 -0.6 7,639 - GFF3235 0.62 +0.9 7,729 + GFF3235 0.66 +1.0 7,730 - GFF3235 0.66 +0.4 7,730 - GFF3235 0.66 -2.4 7,730 - GFF3235 0.66 +0.3 7,734 + GFF3235 0.66 -0.0 7,734 + GFF3235 0.66 -0.9 7,735 - GFF3235 0.66 -1.4 7,811 + GFF3235 0.69 -0.1 7,984 + GFF3235 0.76 +0.0 7,984 + GFF3235 0.76 +0.1 8,094 + GFF3235 0.80 +0.0 8,098 - GFF3235 0.80 -0.6 8,134 - GFF3235 0.81 -0.5 8,217 + GFF3235 0.85 -0.2 8,332 - GFF3235 0.89 +0.5 8,512 + -1.0 8,520 + -0.5 8,605 - +0.2 8,753 - -0.7 8,830 + -2.1 8,831 - -0.4 8,847 + -1.2 8,914 - -0.1 8,982 + GFF3236 0.15 -2.1 9,048 - GFF3236 0.22 -2.9 9,171 + GFF3236 0.34 -1.1 9,229 - GFF3236 0.40 -1.9 9,328 + GFF3236 0.50 -0.8 9,408 - GFF3236 0.57 -1.4 9,453 + GFF3236 0.62 -1.1 9,454 - GFF3236 0.62 -0.4 9,457 + GFF3236 0.62 -2.6 9,457 + GFF3236 0.62 -1.8
Or see this region's nucleotide sequence