Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3196 and GFF3197 overlap by 4 nucleotides GFF3197 and GFF3198 are separated by 144 nucleotides GFF3198 and GFF3199 are separated by 67 nucleotides GFF3199 and GFF3200 are separated by 123 nucleotides GFF3200 and GFF3201 are separated by 38 nucleotides
GFF3196 - Inner membrane protein YqjE, at 202,241 to 202,639
GFF3196
GFF3197 - Inner membrane protein YqjK, at 202,636 to 202,935
GFF3197
GFF3198 - Inner membrane protein YqjF, at 203,080 to 203,565
GFF3198
GFF3199 - Glutathione S-transferase, omega (EC 2.5.1.18), at 203,633 to 204,619
GFF3199
GFF3200 - Integral membrane protein, at 204,743 to 205,108
GFF3200
GFF3201 - LysR-family transcriptional regulator YhaJ, at 205,147 to 206,043
GFF3201
Position (kb) 203
204
205 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 202.767 kb on + strand, within GFF3197 at 202.775 kb on + strand, within GFF3197 at 202.812 kb on - strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 203.281 kb on + strand, within GFF3198 at 203.281 kb on + strand, within GFF3198 at 203.282 kb on - strand, within GFF3198 at 203.327 kb on + strand, within GFF3198 at 203.406 kb on + strand, within GFF3198 at 203.453 kb on + strand, within GFF3198 at 203.454 kb on - strand, within GFF3198 at 203.504 kb on + strand, within GFF3198 at 203.573 kb on - strand at 203.574 kb on + strand at 203.583 kb on - strand at 203.735 kb on - strand, within GFF3199 at 203.990 kb on + strand, within GFF3199 at 204.062 kb on - strand, within GFF3199 at 204.062 kb on - strand, within GFF3199 at 204.093 kb on + strand, within GFF3199 at 204.095 kb on - strand, within GFF3199 at 204.140 kb on + strand, within GFF3199 at 204.141 kb on - strand, within GFF3199 at 204.262 kb on - strand, within GFF3199 at 204.270 kb on + strand, within GFF3199 at 204.270 kb on + strand, within GFF3199 at 204.318 kb on - strand, within GFF3199 at 204.460 kb on + strand, within GFF3199 at 204.461 kb on - strand, within GFF3199 at 204.471 kb on + strand, within GFF3199 at 204.506 kb on - strand, within GFF3199 at 204.809 kb on + strand, within GFF3200 at 204.917 kb on + strand, within GFF3200 at 205.031 kb on - strand, within GFF3200 at 205.060 kb on + strand, within GFF3200 at 205.162 kb on + strand at 205.163 kb on - strand at 205.163 kb on - strand at 205.171 kb on + strand at 205.230 kb on + strand at 205.354 kb on - strand, within GFF3201 at 205.370 kb on - strand, within GFF3201 at 205.523 kb on + strand, within GFF3201 at 205.523 kb on + strand, within GFF3201 at 205.523 kb on + strand, within GFF3201 at 205.524 kb on - strand, within GFF3201 at 205.539 kb on + strand, within GFF3201 at 205.542 kb on - strand, within GFF3201
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 202,767 + GFF3197 0.44 +0.2 202,775 + GFF3197 0.46 -0.9 202,812 - GFF3197 0.59 +0.0 202,842 + GFF3197 0.69 +0.2 202,842 + GFF3197 0.69 +0.2 202,842 + GFF3197 0.69 +0.7 202,843 - GFF3197 0.69 +0.4 202,843 - GFF3197 0.69 -0.4 202,843 - GFF3197 0.69 -1.1 202,843 - GFF3197 0.69 +0.6 203,281 + GFF3198 0.41 -0.1 203,281 + GFF3198 0.41 +0.2 203,282 - GFF3198 0.42 +0.3 203,327 + GFF3198 0.51 -0.4 203,406 + GFF3198 0.67 -0.2 203,453 + GFF3198 0.77 -0.1 203,454 - GFF3198 0.77 -0.7 203,504 + GFF3198 0.87 -0.1 203,573 - +0.1 203,574 + -1.4 203,583 - +0.2 203,735 - GFF3199 0.10 -0.0 203,990 + GFF3199 0.36 +0.0 204,062 - GFF3199 0.43 -1.3 204,062 - GFF3199 0.43 +0.6 204,093 + GFF3199 0.47 -0.7 204,095 - GFF3199 0.47 +0.3 204,140 + GFF3199 0.51 -0.8 204,141 - GFF3199 0.51 -1.0 204,262 - GFF3199 0.64 +0.3 204,270 + GFF3199 0.65 -0.6 204,270 + GFF3199 0.65 -0.0 204,318 - GFF3199 0.69 -0.7 204,460 + GFF3199 0.84 -0.1 204,461 - GFF3199 0.84 -0.3 204,471 + GFF3199 0.85 -0.0 204,506 - GFF3199 0.88 +0.2 204,809 + GFF3200 0.18 +0.1 204,917 + GFF3200 0.48 -0.3 205,031 - GFF3200 0.79 +0.1 205,060 + GFF3200 0.87 -0.3 205,162 + +1.2 205,163 - +0.2 205,163 - -1.2 205,171 + -1.3 205,230 + -0.7 205,354 - GFF3201 0.23 -2.9 205,370 - GFF3201 0.25 -0.2 205,523 + GFF3201 0.42 -0.1 205,523 + GFF3201 0.42 +1.0 205,523 + GFF3201 0.42 +0.6 205,524 - GFF3201 0.42 +0.2 205,539 + GFF3201 0.44 +0.3 205,542 - GFF3201 0.44 +0.3
Or see this region's nucleotide sequence