Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3191 and GFF3192 are separated by 449 nucleotides GFF3192 and GFF3193 are separated by 3 nucleotides GFF3193 and GFF3194 are separated by 144 nucleotides GFF3194 and GFF3195 are separated by 41 nucleotides
GFF3191 - Sodium:dicarboxylate symporter, at 198,635 to 199,879
GFF3191
GFF3192 - DedA family inner membrane protein YqjA, at 200,329 to 200,991
GFF3192
GFF3193 - Uncharacterized protein YqjB, at 200,995 to 201,378
GFF3193
GFF3194 - Periplasmic protein YqjC, at 201,523 to 201,891
GFF3194
GFF3195 - Uncharacterized membrane protein YqjD, at 201,933 to 202,238
GFF3195
Position (kb) 200
201 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 199.444 kb on + strand, within GFF3191 at 199.492 kb on + strand, within GFF3191 at 199.492 kb on + strand, within GFF3191 at 199.497 kb on - strand, within GFF3191 at 199.526 kb on - strand, within GFF3191 at 199.532 kb on - strand, within GFF3191 at 199.583 kb on - strand, within GFF3191 at 199.740 kb on + strand, within GFF3191 at 199.874 kb on - strand at 199.874 kb on - strand at 199.878 kb on - strand at 199.878 kb on - strand at 199.903 kb on + strand at 199.904 kb on - strand at 199.924 kb on - strand at 199.924 kb on - strand at 199.924 kb on - strand at 199.924 kb on - strand at 199.946 kb on - strand at 199.993 kb on + strand at 199.999 kb on + strand at 199.999 kb on + strand at 199.999 kb on + strand at 200.000 kb on - strand at 200.000 kb on - strand at 200.000 kb on - strand at 200.002 kb on - strand at 200.005 kb on + strand at 200.005 kb on + strand at 200.042 kb on + strand at 200.191 kb on + strand at 200.192 kb on - strand at 200.323 kb on + strand at 200.323 kb on + strand at 200.337 kb on - strand at 200.412 kb on - strand, within GFF3192 at 200.414 kb on - strand, within GFF3192 at 200.424 kb on - strand, within GFF3192 at 200.656 kb on + strand, within GFF3192 at 200.657 kb on - strand, within GFF3192 at 200.872 kb on - strand, within GFF3192 at 200.878 kb on + strand, within GFF3192 at 200.878 kb on + strand, within GFF3192 at 200.892 kb on - strand, within GFF3192 at 200.995 kb on - strand at 201.047 kb on + strand, within GFF3193 at 201.201 kb on + strand, within GFF3193 at 201.284 kb on + strand, within GFF3193 at 201.296 kb on - strand, within GFF3193 at 201.439 kb on + strand at 201.443 kb on - strand at 201.443 kb on - strand at 201.565 kb on - strand, within GFF3194 at 201.578 kb on - strand, within GFF3194 at 201.583 kb on - strand, within GFF3194 at 201.583 kb on - strand, within GFF3194 at 201.614 kb on + strand, within GFF3194 at 201.614 kb on + strand, within GFF3194 at 201.627 kb on + strand, within GFF3194 at 201.837 kb on + strand, within GFF3194
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 199,444 + GFF3191 0.65 +0.0 199,492 + GFF3191 0.69 -0.5 199,492 + GFF3191 0.69 -0.1 199,497 - GFF3191 0.69 -0.3 199,526 - GFF3191 0.72 -0.5 199,532 - GFF3191 0.72 -0.1 199,583 - GFF3191 0.76 +0.2 199,740 + GFF3191 0.89 -0.5 199,874 - -0.5 199,874 - +0.1 199,878 - -0.4 199,878 - -1.9 199,903 + +1.8 199,904 - +2.8 199,924 - -0.2 199,924 - +0.1 199,924 - -0.2 199,924 - -1.0 199,946 - -1.1 199,993 + -0.0 199,999 + +0.2 199,999 + -0.1 199,999 + +0.1 200,000 - -0.0 200,000 - +1.0 200,000 - +0.2 200,002 - -1.2 200,005 + -0.6 200,005 + +0.3 200,042 + -0.1 200,191 + -0.3 200,192 - -0.4 200,323 + -0.8 200,323 + -0.7 200,337 - -1.5 200,412 - GFF3192 0.13 -1.5 200,414 - GFF3192 0.13 -1.0 200,424 - GFF3192 0.14 -0.4 200,656 + GFF3192 0.49 +0.3 200,657 - GFF3192 0.49 +0.2 200,872 - GFF3192 0.82 -0.9 200,878 + GFF3192 0.83 -1.3 200,878 + GFF3192 0.83 -2.0 200,892 - GFF3192 0.85 -1.1 200,995 - +1.3 201,047 + GFF3193 0.14 +1.3 201,201 + GFF3193 0.54 +1.2 201,284 + GFF3193 0.75 +1.2 201,296 - GFF3193 0.78 +0.8 201,439 + +0.3 201,443 - -1.1 201,443 - +0.4 201,565 - GFF3194 0.11 -0.9 201,578 - GFF3194 0.15 +0.4 201,583 - GFF3194 0.16 +0.5 201,583 - GFF3194 0.16 +1.0 201,614 + GFF3194 0.25 +0.4 201,614 + GFF3194 0.25 +0.3 201,627 + GFF3194 0.28 +0.5 201,837 + GFF3194 0.85 +0.9
Or see this region's nucleotide sequence