Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3184 and GFF3185 are separated by 170 nucleotides GFF3185 and GFF3186 are separated by 80 nucleotides
GFF3184 - Putrescine aminotransferase (EC 2.6.1.82), at 189,840 to 191,219
GFF3184
GFF3185 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 191,390 to 193,408
GFF3185
GFF3186 - 23S rRNA (guanine-N-2-) -methyltransferase rlmG (EC 2.1.1.-), at 193,489 to 194,625
GFF3186
Position (kb) 191
192
193
194 Strain fitness (log2 ratio) -2
-1
0
1 at 190.439 kb on + strand, within GFF3184 at 190.481 kb on + strand, within GFF3184 at 190.596 kb on + strand, within GFF3184 at 190.609 kb on + strand, within GFF3184 at 190.613 kb on + strand, within GFF3184 at 190.613 kb on + strand, within GFF3184 at 190.613 kb on + strand, within GFF3184 at 190.658 kb on - strand, within GFF3184 at 190.826 kb on + strand, within GFF3184 at 190.827 kb on - strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.003 kb on + strand, within GFF3184 at 191.004 kb on - strand, within GFF3184 at 191.091 kb on - strand at 191.099 kb on + strand at 191.245 kb on + strand at 191.336 kb on - strand at 191.361 kb on - strand at 191.431 kb on + strand at 191.432 kb on - strand at 191.661 kb on + strand, within GFF3185 at 191.662 kb on - strand, within GFF3185 at 191.952 kb on - strand, within GFF3185 at 191.983 kb on - strand, within GFF3185 at 191.983 kb on - strand, within GFF3185 at 192.022 kb on + strand, within GFF3185 at 192.123 kb on + strand, within GFF3185 at 192.127 kb on + strand, within GFF3185 at 192.395 kb on - strand, within GFF3185 at 192.403 kb on + strand, within GFF3185 at 192.680 kb on + strand, within GFF3185 at 192.840 kb on - strand, within GFF3185 at 192.926 kb on + strand, within GFF3185 at 192.927 kb on - strand, within GFF3185 at 192.927 kb on - strand, within GFF3185 at 192.961 kb on + strand, within GFF3185 at 193.049 kb on - strand, within GFF3185 at 193.105 kb on - strand, within GFF3185 at 193.204 kb on - strand, within GFF3185 at 193.313 kb on - strand at 193.462 kb on + strand at 193.465 kb on - strand at 193.615 kb on - strand, within GFF3186 at 193.616 kb on + strand, within GFF3186 at 193.858 kb on + strand, within GFF3186 at 193.859 kb on - strand, within GFF3186 at 193.859 kb on - strand, within GFF3186 at 194.037 kb on + strand, within GFF3186 at 194.097 kb on + strand, within GFF3186 at 194.188 kb on - strand, within GFF3186 at 194.214 kb on + strand, within GFF3186 at 194.368 kb on - strand, within GFF3186
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 190,439 + GFF3184 0.43 -0.2 190,481 + GFF3184 0.46 +0.0 190,596 + GFF3184 0.55 -0.1 190,609 + GFF3184 0.56 -1.1 190,613 + GFF3184 0.56 -1.3 190,613 + GFF3184 0.56 +0.1 190,613 + GFF3184 0.56 -1.9 190,658 - GFF3184 0.59 -1.0 190,826 + GFF3184 0.71 -0.2 190,827 - GFF3184 0.72 +0.1 191,003 + GFF3184 0.84 -0.0 191,003 + GFF3184 0.84 +0.1 191,003 + GFF3184 0.84 -1.2 191,003 + GFF3184 0.84 -1.2 191,004 - GFF3184 0.84 -0.2 191,091 - -0.1 191,099 + -0.0 191,245 + -0.0 191,336 - -0.2 191,361 - +0.6 191,431 + +0.1 191,432 - +0.2 191,661 + GFF3185 0.13 +0.3 191,662 - GFF3185 0.13 -0.2 191,952 - GFF3185 0.28 -0.8 191,983 - GFF3185 0.29 +0.4 191,983 - GFF3185 0.29 -0.4 192,022 + GFF3185 0.31 -1.1 192,123 + GFF3185 0.36 -0.2 192,127 + GFF3185 0.37 +0.5 192,395 - GFF3185 0.50 +0.4 192,403 + GFF3185 0.50 +0.4 192,680 + GFF3185 0.64 +0.3 192,840 - GFF3185 0.72 +0.4 192,926 + GFF3185 0.76 +0.4 192,927 - GFF3185 0.76 +0.1 192,927 - GFF3185 0.76 -0.6 192,961 + GFF3185 0.78 +1.5 193,049 - GFF3185 0.82 +0.3 193,105 - GFF3185 0.85 +0.5 193,204 - GFF3185 0.90 +0.0 193,313 - -0.4 193,462 + +0.4 193,465 - -0.5 193,615 - GFF3186 0.11 -0.6 193,616 + GFF3186 0.11 -0.1 193,858 + GFF3186 0.32 +0.2 193,859 - GFF3186 0.33 -0.7 193,859 - GFF3186 0.33 +0.7 194,037 + GFF3186 0.48 +0.2 194,097 + GFF3186 0.53 +0.1 194,188 - GFF3186 0.61 -1.2 194,214 + GFF3186 0.64 +0.3 194,368 - GFF3186 0.77 -0.1
Or see this region's nucleotide sequence