Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3113 and GFF3114 are separated by 103 nucleotides GFF3114 and GFF3115 overlap by 14 nucleotides GFF3115 and GFF3116 are separated by 123 nucleotides
GFF3113 - Putative ATP-dependent RNA helicase-like protein, at 122,183 to 122,686
GFF3113
GFF3114 - FIG01045310: hypothetical protein, at 122,790 to 123,200
GFF3114
GFF3115 - putative cytoplasmic protein, at 123,187 to 123,591
GFF3115
GFF3116 - FIG00553873: hypothetical protein, at 123,715 to 124,599
GFF3116
Position (kb) 123
124 Strain fitness (log2 ratio) -2
-1
0
1
2 at 122.345 kb on + strand, within GFF3113 at 122.346 kb on - strand, within GFF3113 at 122.476 kb on - strand, within GFF3113 at 122.476 kb on - strand, within GFF3113 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.377 kb on - strand, within GFF3115 at 123.377 kb on - strand, within GFF3115 at 123.515 kb on + strand, within GFF3115 at 123.515 kb on + strand, within GFF3115 at 123.516 kb on - strand, within GFF3115 at 123.602 kb on + strand at 123.606 kb on + strand at 123.725 kb on + strand at 123.726 kb on - strand at 124.075 kb on - strand, within GFF3116 at 124.149 kb on - strand, within GFF3116 at 124.272 kb on + strand, within GFF3116 at 124.339 kb on + strand, within GFF3116 at 124.340 kb on - strand, within GFF3116 at 124.519 kb on + strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.525 kb on + strand at 124.525 kb on + strand at 124.526 kb on - strand at 124.549 kb on + strand at 124.550 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 122,345 + GFF3113 0.32 +0.2 122,346 - GFF3113 0.32 -0.0 122,476 - GFF3113 0.58 +0.5 122,476 - GFF3113 0.58 +0.0 123,376 + GFF3115 0.47 +1.4 123,376 + GFF3115 0.47 +0.6 123,376 + GFF3115 0.47 -0.1 123,376 + GFF3115 0.47 +0.5 123,376 + GFF3115 0.47 +0.4 123,376 + GFF3115 0.47 +0.2 123,377 - GFF3115 0.47 +0.4 123,377 - GFF3115 0.47 +0.2 123,515 + GFF3115 0.81 +1.1 123,515 + GFF3115 0.81 +0.9 123,516 - GFF3115 0.81 +0.3 123,602 + -1.8 123,606 + -1.0 123,725 + -1.0 123,726 - -1.5 124,075 - GFF3116 0.41 +0.5 124,149 - GFF3116 0.49 +0.7 124,272 + GFF3116 0.63 +1.2 124,339 + GFF3116 0.71 -1.5 124,340 - GFF3116 0.71 +0.3 124,519 + +0.3 124,520 - -0.4 124,520 - +0.4 124,520 - -0.1 124,525 + -1.2 124,525 + +1.9 124,526 - +0.1 124,549 + +0.1 124,550 - -0.1
Or see this region's nucleotide sequence