Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3106 and GFF3107 overlap by 11 nucleotides GFF3107 and GFF3108 are separated by 2 nucleotides
GFF3106 - Ni/Fe-hydrogenase 2 B-type cytochrome subunit, at 115,622 to 116,800
GFF3106
GFF3107 - Fe-S-cluster-containing hydrogenase components 1, at 116,790 to 117,776
GFF3107
GFF3108 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 117,779 to 118,897
GFF3108
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1 at 115.864 kb on + strand, within GFF3106 at 115.876 kb on + strand, within GFF3106 at 115.990 kb on - strand, within GFF3106 at 116.045 kb on - strand, within GFF3106 at 116.123 kb on + strand, within GFF3106 at 116.124 kb on - strand, within GFF3106 at 116.184 kb on + strand, within GFF3106 at 116.211 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.379 kb on + strand, within GFF3106 at 116.380 kb on - strand, within GFF3106 at 116.442 kb on + strand, within GFF3106 at 116.442 kb on + strand, within GFF3106 at 116.443 kb on - strand, within GFF3106 at 116.448 kb on + strand, within GFF3106 at 116.480 kb on - strand, within GFF3106 at 116.551 kb on - strand, within GFF3106 at 116.572 kb on - strand, within GFF3106 at 116.719 kb on + strand at 116.719 kb on + strand at 116.758 kb on + strand at 116.883 kb on + strand at 116.884 kb on - strand at 116.884 kb on - strand at 116.894 kb on + strand, within GFF3107 at 116.895 kb on - strand, within GFF3107 at 117.045 kb on - strand, within GFF3107 at 117.045 kb on - strand, within GFF3107 at 117.061 kb on + strand, within GFF3107 at 117.061 kb on + strand, within GFF3107 at 117.296 kb on + strand, within GFF3107 at 117.297 kb on - strand, within GFF3107 at 117.297 kb on - strand, within GFF3107 at 117.301 kb on - strand, within GFF3107 at 117.307 kb on + strand, within GFF3107 at 117.323 kb on + strand, within GFF3107 at 117.324 kb on - strand, within GFF3107 at 117.358 kb on + strand, within GFF3107 at 117.423 kb on - strand, within GFF3107 at 117.440 kb on + strand, within GFF3107 at 117.440 kb on + strand, within GFF3107 at 117.440 kb on + strand, within GFF3107 at 117.441 kb on - strand, within GFF3107 at 117.441 kb on - strand, within GFF3107 at 117.441 kb on - strand, within GFF3107 at 117.457 kb on + strand, within GFF3107 at 117.463 kb on + strand, within GFF3107 at 117.533 kb on - strand, within GFF3107 at 117.653 kb on - strand, within GFF3107 at 117.659 kb on + strand, within GFF3107 at 117.755 kb on + strand at 117.780 kb on + strand at 117.833 kb on + strand at 117.833 kb on + strand at 117.922 kb on + strand, within GFF3108 at 118.001 kb on - strand, within GFF3108 at 118.074 kb on - strand, within GFF3108 at 118.078 kb on + strand, within GFF3108 at 118.079 kb on - strand, within GFF3108 at 118.276 kb on - strand, within GFF3108 at 118.286 kb on - strand, within GFF3108 at 118.512 kb on + strand, within GFF3108 at 118.513 kb on - strand, within GFF3108 at 118.588 kb on + strand, within GFF3108 at 118.588 kb on + strand, within GFF3108 at 118.641 kb on + strand, within GFF3108
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 115,864 + GFF3106 0.21 -0.3 115,876 + GFF3106 0.22 -1.1 115,990 - GFF3106 0.31 -0.3 116,045 - GFF3106 0.36 +1.0 116,123 + GFF3106 0.42 +1.7 116,124 - GFF3106 0.43 -0.1 116,184 + GFF3106 0.48 +0.4 116,211 + GFF3106 0.50 -1.4 116,379 + GFF3106 0.64 -0.1 116,379 + GFF3106 0.64 -0.6 116,379 + GFF3106 0.64 +0.4 116,379 + GFF3106 0.64 -1.3 116,380 - GFF3106 0.64 +0.2 116,442 + GFF3106 0.70 +0.4 116,442 + GFF3106 0.70 -0.1 116,443 - GFF3106 0.70 -0.4 116,448 + GFF3106 0.70 -0.2 116,480 - GFF3106 0.73 -1.1 116,551 - GFF3106 0.79 -0.7 116,572 - GFF3106 0.81 +0.0 116,719 + +0.2 116,719 + +0.6 116,758 + +0.5 116,883 + -0.2 116,884 - -0.1 116,884 - -0.3 116,894 + GFF3107 0.11 -0.3 116,895 - GFF3107 0.11 -0.6 117,045 - GFF3107 0.26 -1.0 117,045 - GFF3107 0.26 -1.1 117,061 + GFF3107 0.27 -1.2 117,061 + GFF3107 0.27 -0.1 117,296 + GFF3107 0.51 +0.2 117,297 - GFF3107 0.51 -1.6 117,297 - GFF3107 0.51 +0.6 117,301 - GFF3107 0.52 +0.2 117,307 + GFF3107 0.52 -1.3 117,323 + GFF3107 0.54 -1.2 117,324 - GFF3107 0.54 +0.9 117,358 + GFF3107 0.58 +0.5 117,423 - GFF3107 0.64 +0.0 117,440 + GFF3107 0.66 +0.5 117,440 + GFF3107 0.66 +0.7 117,440 + GFF3107 0.66 +0.8 117,441 - GFF3107 0.66 +0.7 117,441 - GFF3107 0.66 -1.0 117,441 - GFF3107 0.66 +0.2 117,457 + GFF3107 0.68 +0.1 117,463 + GFF3107 0.68 +0.8 117,533 - GFF3107 0.75 +0.2 117,653 - GFF3107 0.87 -0.9 117,659 + GFF3107 0.88 -2.4 117,755 + +0.2 117,780 + +0.3 117,833 + +0.2 117,833 + -0.2 117,922 + GFF3108 0.13 +0.4 118,001 - GFF3108 0.20 -0.0 118,074 - GFF3108 0.26 -0.4 118,078 + GFF3108 0.27 +0.3 118,079 - GFF3108 0.27 +0.3 118,276 - GFF3108 0.44 +0.2 118,286 - GFF3108 0.45 +0.6 118,512 + GFF3108 0.66 -1.0 118,513 - GFF3108 0.66 +0.3 118,588 + GFF3108 0.72 -0.3 118,588 + GFF3108 0.72 +0.3 118,641 + GFF3108 0.77 +0.6
Or see this region's nucleotide sequence