Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3000

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF2999 and GFF3000 are separated by 47 nucleotidesGFF3000 and GFF3001 overlap by 2 nucleotidesGFF3001 and GFF3002 are separated by 13 nucleotidesGFF3002 and GFF3003 are separated by 225 nucleotides GFF2999 - 6-phospho-beta-glucosidase (EC 3.2.1.86), at 14,441 to 15,874 GFF2999 GFF3000 - Putative uncharacterized protein STY3208 (Putative uncharacterized protein), at 15,922 to 16,656 GFF3000 GFF3001 - hypothetical protein, at 16,655 to 16,885 GFF3001 GFF3002 - hypothetical protein, at 16,899 to 17,045 GFF3002 GFF3003 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 17,271 to 20,144 GFF3003 Position (kb) 15 16 17Strain fitness (log2 ratio) -2 -1 0 1at 14.944 kb on + strand, within GFF2999at 14.945 kb on - strand, within GFF2999at 14.945 kb on - strand, within GFF2999at 14.955 kb on - strand, within GFF2999at 15.035 kb on + strand, within GFF2999at 15.068 kb on + strand, within GFF2999at 15.069 kb on - strand, within GFF2999at 15.078 kb on + strand, within GFF2999at 15.078 kb on + strand, within GFF2999at 15.154 kb on + strand, within GFF2999at 15.154 kb on + strand, within GFF2999at 15.154 kb on + strand, within GFF2999at 15.154 kb on + strand, within GFF2999at 15.177 kb on - strand, within GFF2999at 15.190 kb on + strand, within GFF2999at 15.245 kb on - strand, within GFF2999at 15.278 kb on + strand, within GFF2999at 15.365 kb on + strand, within GFF2999at 15.383 kb on + strand, within GFF2999at 15.462 kb on - strand, within GFF2999at 15.466 kb on + strand, within GFF2999at 15.531 kb on - strand, within GFF2999at 15.540 kb on - strand, within GFF2999at 15.556 kb on + strand, within GFF2999at 15.592 kb on - strand, within GFF2999at 15.746 kb on + strandat 15.771 kb on - strandat 15.771 kb on - strandat 15.771 kb on - strandat 15.873 kb on - strandat 15.938 kb on - strandat 16.050 kb on + strand, within GFF3000at 16.095 kb on + strand, within GFF3000at 16.095 kb on + strand, within GFF3000at 16.096 kb on - strand, within GFF3000at 16.110 kb on + strand, within GFF3000at 16.137 kb on + strand, within GFF3000at 16.189 kb on - strand, within GFF3000at 16.345 kb on - strand, within GFF3000at 16.362 kb on + strand, within GFF3000at 16.384 kb on + strand, within GFF3000at 16.402 kb on + strand, within GFF3000at 16.522 kb on - strand, within GFF3000at 16.523 kb on + strand, within GFF3000at 17.267 kb on + strandat 17.272 kb on + strandat 17.357 kb on - strandat 17.552 kb on + strandat 17.553 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
remove
14,944 + GFF2999 0.35 -0.2
14,945 - GFF2999 0.35 -0.4
14,945 - GFF2999 0.35 +0.7
14,955 - GFF2999 0.36 +0.2
15,035 + GFF2999 0.41 -1.2
15,068 + GFF2999 0.44 +0.5
15,069 - GFF2999 0.44 +0.9
15,078 + GFF2999 0.44 -1.0
15,078 + GFF2999 0.44 +0.1
15,154 + GFF2999 0.50 +0.2
15,154 + GFF2999 0.50 +0.4
15,154 + GFF2999 0.50 -2.1
15,154 + GFF2999 0.50 -0.3
15,177 - GFF2999 0.51 -1.7
15,190 + GFF2999 0.52 -0.1
15,245 - GFF2999 0.56 -0.2
15,278 + GFF2999 0.58 +0.1
15,365 + GFF2999 0.64 -1.5
15,383 + GFF2999 0.66 +0.3
15,462 - GFF2999 0.71 +1.6
15,466 + GFF2999 0.71 -0.6
15,531 - GFF2999 0.76 +0.1
15,540 - GFF2999 0.77 -0.1
15,556 + GFF2999 0.78 -0.0
15,592 - GFF2999 0.80 -0.0
15,746 + -1.0
15,771 - +0.6
15,771 - +0.6
15,771 - -0.1
15,873 - +0.5
15,938 - -0.8
16,050 + GFF3000 0.17 +0.1
16,095 + GFF3000 0.24 +0.5
16,095 + GFF3000 0.24 +1.0
16,096 - GFF3000 0.24 -0.0
16,110 + GFF3000 0.26 +0.4
16,137 + GFF3000 0.29 +0.9
16,189 - GFF3000 0.36 +0.2
16,345 - GFF3000 0.58 -0.0
16,362 + GFF3000 0.60 -1.1
16,384 + GFF3000 0.63 +0.4
16,402 + GFF3000 0.65 +0.3
16,522 - GFF3000 0.82 +0.1
16,523 + GFF3000 0.82 +0.6
17,267 + -1.9
17,272 + +0.8
17,357 - +0.2
17,552 + -0.9
17,553 - +0.1

Or see this region's nucleotide sequence