Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2999 and GFF3000 are separated by 47 nucleotides GFF3000 and GFF3001 overlap by 2 nucleotides GFF3001 and GFF3002 are separated by 13 nucleotides GFF3002 and GFF3003 are separated by 225 nucleotides
GFF2999 - 6-phospho-beta-glucosidase (EC 3.2.1.86), at 14,441 to 15,874
GFF2999
GFF3000 - Putative uncharacterized protein STY3208 (Putative uncharacterized protein), at 15,922 to 16,656
GFF3000
GFF3001 - hypothetical protein, at 16,655 to 16,885
GFF3001
GFF3002 - hypothetical protein, at 16,899 to 17,045
GFF3002
GFF3003 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 17,271 to 20,144
GFF3003
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-2
-1
0
1 at 14.944 kb on + strand, within GFF2999 at 14.945 kb on - strand, within GFF2999 at 14.945 kb on - strand, within GFF2999 at 14.955 kb on - strand, within GFF2999 at 15.035 kb on + strand, within GFF2999 at 15.068 kb on + strand, within GFF2999 at 15.069 kb on - strand, within GFF2999 at 15.078 kb on + strand, within GFF2999 at 15.078 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.154 kb on + strand, within GFF2999 at 15.177 kb on - strand, within GFF2999 at 15.190 kb on + strand, within GFF2999 at 15.245 kb on - strand, within GFF2999 at 15.278 kb on + strand, within GFF2999 at 15.365 kb on + strand, within GFF2999 at 15.383 kb on + strand, within GFF2999 at 15.462 kb on - strand, within GFF2999 at 15.466 kb on + strand, within GFF2999 at 15.531 kb on - strand, within GFF2999 at 15.540 kb on - strand, within GFF2999 at 15.556 kb on + strand, within GFF2999 at 15.592 kb on - strand, within GFF2999 at 15.746 kb on + strand at 15.771 kb on - strand at 15.771 kb on - strand at 15.771 kb on - strand at 15.873 kb on - strand at 15.938 kb on - strand at 16.050 kb on + strand, within GFF3000 at 16.095 kb on + strand, within GFF3000 at 16.095 kb on + strand, within GFF3000 at 16.096 kb on - strand, within GFF3000 at 16.110 kb on + strand, within GFF3000 at 16.137 kb on + strand, within GFF3000 at 16.189 kb on - strand, within GFF3000 at 16.345 kb on - strand, within GFF3000 at 16.362 kb on + strand, within GFF3000 at 16.384 kb on + strand, within GFF3000 at 16.402 kb on + strand, within GFF3000 at 16.522 kb on - strand, within GFF3000 at 16.523 kb on + strand, within GFF3000 at 17.267 kb on + strand at 17.272 kb on + strand at 17.357 kb on - strand at 17.552 kb on + strand at 17.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 14,944 + GFF2999 0.35 -0.2 14,945 - GFF2999 0.35 -0.4 14,945 - GFF2999 0.35 +0.7 14,955 - GFF2999 0.36 +0.2 15,035 + GFF2999 0.41 -1.2 15,068 + GFF2999 0.44 +0.5 15,069 - GFF2999 0.44 +0.9 15,078 + GFF2999 0.44 -1.0 15,078 + GFF2999 0.44 +0.1 15,154 + GFF2999 0.50 +0.2 15,154 + GFF2999 0.50 +0.4 15,154 + GFF2999 0.50 -2.1 15,154 + GFF2999 0.50 -0.3 15,177 - GFF2999 0.51 -1.7 15,190 + GFF2999 0.52 -0.1 15,245 - GFF2999 0.56 -0.2 15,278 + GFF2999 0.58 +0.1 15,365 + GFF2999 0.64 -1.5 15,383 + GFF2999 0.66 +0.3 15,462 - GFF2999 0.71 +1.6 15,466 + GFF2999 0.71 -0.6 15,531 - GFF2999 0.76 +0.1 15,540 - GFF2999 0.77 -0.1 15,556 + GFF2999 0.78 -0.0 15,592 - GFF2999 0.80 -0.0 15,746 + -1.0 15,771 - +0.6 15,771 - +0.6 15,771 - -0.1 15,873 - +0.5 15,938 - -0.8 16,050 + GFF3000 0.17 +0.1 16,095 + GFF3000 0.24 +0.5 16,095 + GFF3000 0.24 +1.0 16,096 - GFF3000 0.24 -0.0 16,110 + GFF3000 0.26 +0.4 16,137 + GFF3000 0.29 +0.9 16,189 - GFF3000 0.36 +0.2 16,345 - GFF3000 0.58 -0.0 16,362 + GFF3000 0.60 -1.1 16,384 + GFF3000 0.63 +0.4 16,402 + GFF3000 0.65 +0.3 16,522 - GFF3000 0.82 +0.1 16,523 + GFF3000 0.82 +0.6 17,267 + -1.9 17,272 + +0.8 17,357 - +0.2 17,552 + -0.9 17,553 - +0.1
Or see this region's nucleotide sequence