Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2901

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2900 and GFF2901 are separated by 2 nucleotidesGFF2901 and GFF2902 are separated by 160 nucleotides GFF2900 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 25,806 to 27,350 GFF2900 GFF2901 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 27,353 to 29,203 GFF2901 GFF2902 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 29,364 to 30,293 GFF2902 Position (kb) 27 28 29 30Strain fitness (log2 ratio) -1 0 1 2at 26.375 kb on - strand, within GFF2900at 26.456 kb on - strand, within GFF2900at 26.543 kb on + strand, within GFF2900at 26.562 kb on - strand, within GFF2900at 26.562 kb on - strand, within GFF2900at 26.562 kb on - strand, within GFF2900at 26.562 kb on - strand, within GFF2900at 26.562 kb on - strand, within GFF2900at 26.564 kb on + strand, within GFF2900at 26.626 kb on - strand, within GFF2900at 27.167 kb on + strand, within GFF2900at 27.168 kb on - strand, within GFF2900at 27.173 kb on + strand, within GFF2900at 27.174 kb on - strand, within GFF2900at 27.174 kb on - strand, within GFF2900at 27.174 kb on - strand, within GFF2900at 27.174 kb on - strand, within GFF2900at 27.178 kb on - strand, within GFF2900at 27.275 kb on - strandat 27.275 kb on - strandat 27.289 kb on - strandat 27.592 kb on + strand, within GFF2901at 27.723 kb on + strand, within GFF2901at 27.841 kb on - strand, within GFF2901at 27.957 kb on + strand, within GFF2901at 27.958 kb on - strand, within GFF2901at 27.958 kb on - strand, within GFF2901at 27.964 kb on - strand, within GFF2901at 27.964 kb on - strand, within GFF2901at 28.043 kb on - strand, within GFF2901at 28.114 kb on - strand, within GFF2901at 28.114 kb on - strand, within GFF2901at 28.238 kb on - strand, within GFF2901at 28.242 kb on + strand, within GFF2901at 28.243 kb on - strand, within GFF2901at 28.268 kb on - strand, within GFF2901at 28.433 kb on - strand, within GFF2901at 28.468 kb on - strand, within GFF2901at 28.519 kb on + strand, within GFF2901at 28.850 kb on - strand, within GFF2901at 28.930 kb on - strand, within GFF2901at 28.936 kb on + strand, within GFF2901at 29.162 kb on - strandat 29.164 kb on + strandat 29.193 kb on + strandat 29.197 kb on + strandat 29.197 kb on + strandat 29.198 kb on - strandat 29.198 kb on - strandat 29.234 kb on + strandat 29.234 kb on + strandat 29.235 kb on - strandat 29.281 kb on + strandat 29.289 kb on + strandat 29.289 kb on + strandat 29.290 kb on - strandat 29.532 kb on - strand, within GFF2902at 29.591 kb on + strand, within GFF2902at 29.730 kb on + strand, within GFF2902at 29.800 kb on + strand, within GFF2902at 29.905 kb on + strand, within GFF2902at 29.906 kb on - strand, within GFF2902at 29.948 kb on - strand, within GFF2902at 29.991 kb on + strand, within GFF2902at 30.007 kb on + strand, within GFF2902at 30.007 kb on + strand, within GFF2902at 30.007 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.123 kb on - strand, within GFF2902at 30.123 kb on - strand, within GFF2902at 30.195 kb on - strand, within GFF2902

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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26,375 - GFF2900 0.37 +2.0
26,456 - GFF2900 0.42 -0.2
26,543 + GFF2900 0.48 +1.9
26,562 - GFF2900 0.49 -0.1
26,562 - GFF2900 0.49 +0.4
26,562 - GFF2900 0.49 +0.2
26,562 - GFF2900 0.49 +0.3
26,562 - GFF2900 0.49 +0.7
26,564 + GFF2900 0.49 -0.5
26,626 - GFF2900 0.53 +0.5
27,167 + GFF2900 0.88 +0.6
27,168 - GFF2900 0.88 +0.8
27,173 + GFF2900 0.88 -0.6
27,174 - GFF2900 0.89 +0.4
27,174 - GFF2900 0.89 +0.0
27,174 - GFF2900 0.89 +0.7
27,174 - GFF2900 0.89 +0.4
27,178 - GFF2900 0.89 -0.4
27,275 - -0.0
27,275 - -0.5
27,289 - +0.5
27,592 + GFF2901 0.13 +0.2
27,723 + GFF2901 0.20 +0.5
27,841 - GFF2901 0.26 +1.3
27,957 + GFF2901 0.33 +0.4
27,958 - GFF2901 0.33 -0.6
27,958 - GFF2901 0.33 -0.1
27,964 - GFF2901 0.33 +1.6
27,964 - GFF2901 0.33 +0.2
28,043 - GFF2901 0.37 +0.4
28,114 - GFF2901 0.41 +0.7
28,114 - GFF2901 0.41 +0.0
28,238 - GFF2901 0.48 +0.7
28,242 + GFF2901 0.48 -1.0
28,243 - GFF2901 0.48 +0.6
28,268 - GFF2901 0.49 +0.2
28,433 - GFF2901 0.58 +1.1
28,468 - GFF2901 0.60 +0.0
28,519 + GFF2901 0.63 +0.3
28,850 - GFF2901 0.81 +0.7
28,930 - GFF2901 0.85 +0.9
28,936 + GFF2901 0.86 +1.0
29,162 - +0.5
29,164 + +0.5
29,193 + -0.2
29,197 + -0.8
29,197 + +0.9
29,198 - +0.7
29,198 - +0.2
29,234 + +1.3
29,234 + +0.1
29,235 - -0.8
29,281 + -1.6
29,289 + -0.9
29,289 + -1.0
29,290 - +0.4
29,532 - GFF2902 0.18 +0.6
29,591 + GFF2902 0.24 +0.6
29,730 + GFF2902 0.39 +0.4
29,800 + GFF2902 0.47 +0.3
29,905 + GFF2902 0.58 -1.7
29,906 - GFF2902 0.58 -0.1
29,948 - GFF2902 0.63 +0.6
29,991 + GFF2902 0.67 +0.9
30,007 + GFF2902 0.69 +0.3
30,007 + GFF2902 0.69 -0.5
30,007 + GFF2902 0.69 +0.4
30,122 + GFF2902 0.82 -1.3
30,122 + GFF2902 0.82 +0.7
30,122 + GFF2902 0.82 +0.6
30,122 + GFF2902 0.82 +0.5
30,123 - GFF2902 0.82 +0.7
30,123 - GFF2902 0.82 +0.3
30,195 - GFF2902 0.89 +0.5

Or see this region's nucleotide sequence