Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2789 and GFF2790 overlap by 4 nucleotides GFF2790 and GFF2791 overlap by 11 nucleotides GFF2791 and GFF2792 overlap by 4 nucleotides GFF2792 and GFF2793 are separated by 74 nucleotides
GFF2789 - Ni,Fe-hydrogenase I cytochrome b subunit, at 313,024 to 313,755
GFF2789
GFF2790 - Hydrogenase maturation protease (EC 3.4.24.-), at 313,752 to 314,348
GFF2790
GFF2791 - Hydrogenase maturation factor hoxO, at 314,338 to 314,742
GFF2791
GFF2792 - Hydrogenase maturation factor HoxQ, at 314,739 to 315,587
GFF2792
GFF2793 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 315,662 to 317,206
GFF2793
Position (kb) 314
315
316 Strain fitness (log2 ratio) -1
0
1
2 at 313.911 kb on + strand, within GFF2790 at 313.912 kb on - strand, within GFF2790 at 314.262 kb on + strand, within GFF2790 at 314.264 kb on + strand, within GFF2790 at 314.265 kb on - strand, within GFF2790 at 314.302 kb on + strand at 314.302 kb on + strand at 314.302 kb on + strand at 314.413 kb on - strand, within GFF2791 at 314.459 kb on + strand, within GFF2791 at 314.599 kb on - strand, within GFF2791 at 314.604 kb on - strand, within GFF2791 at 314.736 kb on - strand at 314.775 kb on - strand at 314.846 kb on + strand, within GFF2792 at 314.962 kb on + strand, within GFF2792 at 314.963 kb on - strand, within GFF2792 at 314.999 kb on + strand, within GFF2792 at 315.479 kb on + strand, within GFF2792 at 315.483 kb on + strand, within GFF2792 at 315.484 kb on - strand, within GFF2792 at 315.626 kb on + strand at 315.779 kb on + strand at 315.779 kb on + strand at 315.780 kb on - strand at 315.780 kb on - strand at 315.898 kb on + strand, within GFF2793 at 315.911 kb on + strand, within GFF2793 at 315.921 kb on - strand, within GFF2793 at 316.027 kb on + strand, within GFF2793 at 316.235 kb on + strand, within GFF2793
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 313,911 + GFF2790 0.27 -0.4 313,912 - GFF2790 0.27 -0.1 314,262 + GFF2790 0.85 +0.2 314,264 + GFF2790 0.86 +0.2 314,265 - GFF2790 0.86 +1.0 314,302 + -0.9 314,302 + -1.0 314,302 + +0.6 314,413 - GFF2791 0.19 -0.0 314,459 + GFF2791 0.30 -0.6 314,599 - GFF2791 0.64 -0.7 314,604 - GFF2791 0.66 -0.2 314,736 - +0.0 314,775 - -0.3 314,846 + GFF2792 0.13 +0.0 314,962 + GFF2792 0.26 +0.1 314,963 - GFF2792 0.26 +1.1 314,999 + GFF2792 0.31 +2.4 315,479 + GFF2792 0.87 +0.1 315,483 + GFF2792 0.88 -0.6 315,484 - GFF2792 0.88 -0.2 315,626 + -0.7 315,779 + +0.1 315,779 + -0.7 315,780 - -0.2 315,780 - -1.6 315,898 + GFF2793 0.15 -0.1 315,911 + GFF2793 0.16 +0.5 315,921 - GFF2793 0.17 +0.2 316,027 + GFF2793 0.24 -1.1 316,235 + GFF2793 0.37 -0.2
Or see this region's nucleotide sequence