Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF277

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF276 and GFF277 are separated by 11 nucleotides GFF276 - Potassium uptake protein TrkH, at 52,507 to 53,958 GFF276 GFF277 - Protoporphyrinogen IX oxidase, oxygen-independent, HemG (EC 1.3.-.-), at 53,970 to 54,515 GFF277 Position (kb) 53 54 55Strain fitness (log2 ratio) -1 0 1 2 3at 53.067 kb on + strand, within GFF276at 53.067 kb on + strand, within GFF276at 53.071 kb on + strand, within GFF276at 53.072 kb on - strand, within GFF276at 53.072 kb on - strand, within GFF276at 53.218 kb on - strand, within GFF276at 53.260 kb on - strand, within GFF276at 53.282 kb on + strand, within GFF276at 53.283 kb on - strand, within GFF276at 53.395 kb on - strand, within GFF276at 53.398 kb on + strand, within GFF276at 53.520 kb on - strand, within GFF276at 53.567 kb on + strand, within GFF276at 53.649 kb on + strand, within GFF276at 53.652 kb on + strand, within GFF276at 53.653 kb on - strand, within GFF276at 53.653 kb on - strand, within GFF276at 53.738 kb on + strand, within GFF276at 53.740 kb on - strand, within GFF276at 53.751 kb on + strand, within GFF276at 53.805 kb on + strand, within GFF276at 53.879 kb on + strandat 53.913 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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53,067 + GFF276 0.39 +1.9
53,067 + GFF276 0.39 +2.0
53,071 + GFF276 0.39 +3.1
53,072 - GFF276 0.39 +0.7
53,072 - GFF276 0.39 +1.6
53,218 - GFF276 0.49 +2.4
53,260 - GFF276 0.52 +1.7
53,282 + GFF276 0.53 +1.9
53,283 - GFF276 0.53 +1.3
53,395 - GFF276 0.61 +1.7
53,398 + GFF276 0.61 +1.5
53,520 - GFF276 0.70 +1.4
53,567 + GFF276 0.73 -1.4
53,649 + GFF276 0.79 +1.9
53,652 + GFF276 0.79 +0.7
53,653 - GFF276 0.79 +1.3
53,653 - GFF276 0.79 +1.8
53,738 + GFF276 0.85 +1.4
53,740 - GFF276 0.85 +2.2
53,751 + GFF276 0.86 +0.6
53,805 + GFF276 0.89 +2.9
53,879 + +1.2
53,913 - +1.5

Or see this region's nucleotide sequence