Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2449 and GFF2450 are separated by 181 nucleotides GFF2450 and GFF2451 are separated by 360 nucleotides
GFF2449 - Flavohemoprotein (Hemoglobin-like protein) (Flavohemoglobin) (Nitric oxide dioxygenase) (EC 1.14.12.17), at 63,744 to 64,934
GFF2449
GFF2450 - Transcriptional activator of cad operon, at 65,116 to 66,660
GFF2450
GFF2451 - Lysine/cadaverine antiporter membrane protein CadB, at 67,021 to 68,352
GFF2451
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1 at 64.121 kb on - strand, within GFF2449 at 64.306 kb on - strand, within GFF2449 at 64.682 kb on + strand, within GFF2449 at 64.706 kb on + strand, within GFF2449 at 64.777 kb on + strand, within GFF2449 at 64.822 kb on - strand at 65.024 kb on - strand at 65.133 kb on - strand at 65.196 kb on + strand at 65.231 kb on - strand at 65.351 kb on + strand, within GFF2450 at 65.352 kb on - strand, within GFF2450 at 65.409 kb on + strand, within GFF2450 at 65.445 kb on + strand, within GFF2450 at 65.673 kb on + strand, within GFF2450 at 65.719 kb on - strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.720 kb on + strand, within GFF2450 at 65.754 kb on + strand, within GFF2450 at 65.776 kb on + strand, within GFF2450 at 65.822 kb on - strand, within GFF2450 at 65.832 kb on + strand, within GFF2450 at 65.996 kb on - strand, within GFF2450 at 66.062 kb on + strand, within GFF2450 at 66.160 kb on - strand, within GFF2450 at 66.217 kb on - strand, within GFF2450 at 66.241 kb on + strand, within GFF2450 at 66.242 kb on - strand, within GFF2450 at 67.184 kb on - strand, within GFF2451 at 67.324 kb on - strand, within GFF2451 at 67.596 kb on + strand, within GFF2451
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 64,121 - GFF2449 0.32 -1.0 64,306 - GFF2449 0.47 +0.2 64,682 + GFF2449 0.79 -0.6 64,706 + GFF2449 0.81 -0.1 64,777 + GFF2449 0.87 +0.5 64,822 - -0.8 65,024 - -0.2 65,133 - -0.1 65,196 + +0.2 65,231 - +0.2 65,351 + GFF2450 0.15 +0.1 65,352 - GFF2450 0.15 +0.0 65,409 + GFF2450 0.19 -0.0 65,445 + GFF2450 0.21 -0.2 65,673 + GFF2450 0.36 -1.3 65,719 - GFF2450 0.39 +0.1 65,720 + GFF2450 0.39 -0.5 65,720 + GFF2450 0.39 -0.0 65,720 + GFF2450 0.39 +0.1 65,720 + GFF2450 0.39 -0.4 65,720 + GFF2450 0.39 -0.7 65,720 + GFF2450 0.39 -1.3 65,720 + GFF2450 0.39 -0.3 65,754 + GFF2450 0.41 -0.1 65,776 + GFF2450 0.43 +0.3 65,822 - GFF2450 0.46 -0.0 65,832 + GFF2450 0.46 +0.2 65,996 - GFF2450 0.57 -0.3 66,062 + GFF2450 0.61 +0.4 66,160 - GFF2450 0.68 +0.2 66,217 - GFF2450 0.71 -0.8 66,241 + GFF2450 0.73 -0.5 66,242 - GFF2450 0.73 -1.0 67,184 - GFF2451 0.12 -0.5 67,324 - GFF2451 0.23 -0.2 67,596 + GFF2451 0.43 -1.1
Or see this region's nucleotide sequence