Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2286 and GFF2287 are separated by 10 nucleotides GFF2287 and GFF2288 are separated by 9 nucleotides GFF2288 and GFF2289 are separated by 62 nucleotides GFF2289 and GFF2290 are separated by 97 nucleotides
GFF2286 - Melibiose carrier protein, Na+/melibiose symporter, at 74,528 to 75,862
GFF2286
GFF2287 - Fructokinase (EC 2.7.1.4), at 75,873 to 76,832
GFF2287
GFF2288 - ADP-ribosylglycohydrolase (EC 3.2.-.-), at 76,842 to 77,882
GFF2288
GFF2289 - Transcriptional regulator, GntR family, at 77,945 to 78,667
GFF2289
GFF2290 - Putative periplasmic protein, at 78,765 to 78,929
GFF2290
Position (kb) 76
77
78 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 76.173 kb on + strand, within GFF2287 at 76.174 kb on - strand, within GFF2287 at 76.174 kb on - strand, within GFF2287 at 76.196 kb on + strand, within GFF2287 at 76.355 kb on - strand, within GFF2287 at 76.424 kb on + strand, within GFF2287 at 76.559 kb on + strand, within GFF2287 at 76.610 kb on + strand, within GFF2287 at 76.610 kb on + strand, within GFF2287 at 76.762 kb on + strand at 76.939 kb on + strand at 77.049 kb on + strand, within GFF2288 at 77.049 kb on + strand, within GFF2288 at 77.049 kb on + strand, within GFF2288 at 77.050 kb on - strand, within GFF2288 at 77.050 kb on - strand, within GFF2288 at 77.139 kb on + strand, within GFF2288 at 77.242 kb on + strand, within GFF2288 at 77.327 kb on + strand, within GFF2288 at 77.478 kb on + strand, within GFF2288 at 77.478 kb on + strand, within GFF2288 at 77.479 kb on - strand, within GFF2288 at 77.479 kb on - strand, within GFF2288 at 77.570 kb on - strand, within GFF2288 at 77.712 kb on - strand, within GFF2288 at 77.714 kb on + strand, within GFF2288 at 77.714 kb on + strand, within GFF2288 at 77.714 kb on + strand, within GFF2288 at 77.741 kb on + strand, within GFF2288 at 77.979 kb on - strand at 77.981 kb on - strand at 78.045 kb on + strand, within GFF2289 at 78.134 kb on - strand, within GFF2289 at 78.191 kb on + strand, within GFF2289 at 78.192 kb on - strand, within GFF2289 at 78.211 kb on + strand, within GFF2289 at 78.330 kb on - strand, within GFF2289 at 78.330 kb on - strand, within GFF2289 at 78.337 kb on + strand, within GFF2289 at 78.337 kb on + strand, within GFF2289 at 78.373 kb on + strand, within GFF2289 at 78.388 kb on - strand, within GFF2289 at 78.389 kb on + strand, within GFF2289 at 78.445 kb on + strand, within GFF2289 at 78.445 kb on + strand, within GFF2289 at 78.446 kb on - strand, within GFF2289 at 78.446 kb on - strand, within GFF2289 at 78.465 kb on + strand, within GFF2289 at 78.554 kb on - strand, within GFF2289 at 78.700 kb on + strand at 78.701 kb on - strand at 78.712 kb on - strand at 78.764 kb on + strand at 78.765 kb on - strand at 78.765 kb on - strand at 78.819 kb on - strand, within GFF2290
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 76,173 + GFF2287 0.31 -0.4 76,174 - GFF2287 0.31 -0.3 76,174 - GFF2287 0.31 -1.4 76,196 + GFF2287 0.34 -0.2 76,355 - GFF2287 0.50 -0.1 76,424 + GFF2287 0.57 +0.2 76,559 + GFF2287 0.71 +0.4 76,610 + GFF2287 0.77 -0.8 76,610 + GFF2287 0.77 -1.6 76,762 + -0.5 76,939 + -0.1 77,049 + GFF2288 0.20 -0.4 77,049 + GFF2288 0.20 +0.3 77,049 + GFF2288 0.20 +0.4 77,050 - GFF2288 0.20 -1.1 77,050 - GFF2288 0.20 +0.3 77,139 + GFF2288 0.29 +0.0 77,242 + GFF2288 0.38 +0.7 77,327 + GFF2288 0.47 -0.9 77,478 + GFF2288 0.61 +0.8 77,478 + GFF2288 0.61 +0.6 77,479 - GFF2288 0.61 -0.4 77,479 - GFF2288 0.61 +0.0 77,570 - GFF2288 0.70 -0.3 77,712 - GFF2288 0.84 -2.4 77,714 + GFF2288 0.84 -0.8 77,714 + GFF2288 0.84 -0.5 77,714 + GFF2288 0.84 -0.4 77,741 + GFF2288 0.86 -1.6 77,979 - +0.1 77,981 - +0.4 78,045 + GFF2289 0.14 -1.8 78,134 - GFF2289 0.26 +0.6 78,191 + GFF2289 0.34 -0.1 78,192 - GFF2289 0.34 -1.2 78,211 + GFF2289 0.37 +0.3 78,330 - GFF2289 0.53 +0.1 78,330 - GFF2289 0.53 -4.0 78,337 + GFF2289 0.54 +0.6 78,337 + GFF2289 0.54 -0.2 78,373 + GFF2289 0.59 -0.6 78,388 - GFF2289 0.61 -1.3 78,389 + GFF2289 0.61 +0.0 78,445 + GFF2289 0.69 -0.1 78,445 + GFF2289 0.69 -0.2 78,446 - GFF2289 0.69 -1.3 78,446 - GFF2289 0.69 +0.3 78,465 + GFF2289 0.72 +1.1 78,554 - GFF2289 0.84 +0.1 78,700 + +0.8 78,701 - +0.5 78,712 - +0.1 78,764 + +0.4 78,765 - +0.6 78,765 - +0.2 78,819 - GFF2290 0.33 +0.3
Or see this region's nucleotide sequence