Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2284 and GFF2285 are separated by 100 nucleotides GFF2285 and GFF2286 are separated by 262 nucleotides
GFF2284 - Sulfate-binding protein Sbp, at 72,420 to 73,409
GFF2284
GFF2285 - CDP-diacylglycerol pyrophosphatase (EC 3.6.1.26), at 73,510 to 74,265
GFF2285
GFF2286 - Melibiose carrier protein, Na+/melibiose symporter, at 74,528 to 75,862
GFF2286
Position (kb) 73
74
75 Strain fitness (log2 ratio) -1
0
1
2 at 72.635 kb on + strand, within GFF2284 at 72.753 kb on + strand, within GFF2284 at 72.754 kb on - strand, within GFF2284 at 73.010 kb on + strand, within GFF2284 at 73.010 kb on + strand, within GFF2284 at 73.089 kb on - strand, within GFF2284 at 73.095 kb on - strand, within GFF2284 at 73.095 kb on - strand, within GFF2284 at 73.201 kb on - strand, within GFF2284 at 73.418 kb on + strand at 73.433 kb on - strand at 73.506 kb on + strand at 73.643 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.733 kb on + strand, within GFF2285 at 73.747 kb on + strand, within GFF2285 at 73.752 kb on + strand, within GFF2285 at 73.832 kb on - strand, within GFF2285 at 73.833 kb on + strand, within GFF2285 at 73.834 kb on - strand, within GFF2285 at 73.834 kb on - strand, within GFF2285 at 73.858 kb on - strand, within GFF2285 at 74.083 kb on - strand, within GFF2285 at 74.147 kb on + strand, within GFF2285 at 74.197 kb on - strand at 74.238 kb on + strand at 74.238 kb on + strand at 74.238 kb on + strand at 74.239 kb on - strand at 74.295 kb on - strand at 74.295 kb on - strand at 74.402 kb on + strand at 74.461 kb on + strand at 74.618 kb on + strand at 74.619 kb on - strand at 74.624 kb on + strand at 74.624 kb on + strand at 74.625 kb on - strand at 74.665 kb on - strand, within GFF2286 at 74.665 kb on - strand, within GFF2286 at 74.832 kb on - strand, within GFF2286 at 74.832 kb on - strand, within GFF2286 at 74.833 kb on + strand, within GFF2286 at 74.834 kb on - strand, within GFF2286 at 74.856 kb on - strand, within GFF2286 at 74.856 kb on - strand, within GFF2286 at 74.864 kb on + strand, within GFF2286 at 74.865 kb on - strand, within GFF2286 at 74.865 kb on - strand, within GFF2286 at 74.867 kb on - strand, within GFF2286 at 74.873 kb on - strand, within GFF2286 at 74.873 kb on - strand, within GFF2286 at 74.885 kb on + strand, within GFF2286 at 74.885 kb on + strand, within GFF2286 at 75.109 kb on + strand, within GFF2286
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 72,635 + GFF2284 0.22 -0.6 72,753 + GFF2284 0.34 +0.4 72,754 - GFF2284 0.34 -0.7 73,010 + GFF2284 0.60 -0.5 73,010 + GFF2284 0.60 -0.2 73,089 - GFF2284 0.68 -0.1 73,095 - GFF2284 0.68 +0.1 73,095 - GFF2284 0.68 +1.1 73,201 - GFF2284 0.79 -1.1 73,418 + +0.6 73,433 - +0.2 73,506 + +1.5 73,643 + GFF2285 0.18 +1.1 73,733 + GFF2285 0.29 +1.4 73,733 + GFF2285 0.29 +1.1 73,733 + GFF2285 0.29 +0.8 73,747 + GFF2285 0.31 +1.7 73,752 + GFF2285 0.32 +0.8 73,832 - GFF2285 0.43 +0.1 73,833 + GFF2285 0.43 +0.8 73,834 - GFF2285 0.43 +0.6 73,834 - GFF2285 0.43 +0.8 73,858 - GFF2285 0.46 -0.4 74,083 - GFF2285 0.76 -0.5 74,147 + GFF2285 0.84 -0.7 74,197 - +1.8 74,238 + +0.8 74,238 + +1.9 74,238 + +0.7 74,239 - +2.2 74,295 - +1.1 74,295 - +0.6 74,402 + +0.4 74,461 + -0.2 74,618 + -0.1 74,619 - +0.0 74,624 + +0.0 74,624 + +0.8 74,625 - +0.3 74,665 - GFF2286 0.10 +1.8 74,665 - GFF2286 0.10 -0.9 74,832 - GFF2286 0.23 -1.4 74,832 - GFF2286 0.23 -0.3 74,833 + GFF2286 0.23 -0.4 74,834 - GFF2286 0.23 +0.7 74,856 - GFF2286 0.25 +0.7 74,856 - GFF2286 0.25 -0.0 74,864 + GFF2286 0.25 -1.4 74,865 - GFF2286 0.25 -0.0 74,865 - GFF2286 0.25 -0.2 74,867 - GFF2286 0.25 +0.1 74,873 - GFF2286 0.26 -1.2 74,873 - GFF2286 0.26 -0.3 74,885 + GFF2286 0.27 +0.1 74,885 + GFF2286 0.27 +0.9 75,109 + GFF2286 0.44 +0.0
Or see this region's nucleotide sequence