Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2183 and GFF2184 are separated by 15 nucleotides GFF2184 and GFF2185 are separated by 26 nucleotides GFF2185 and GFF2186 are separated by 27 nucleotides
GFF2183 - YpfJ protein, zinc metalloprotease superfamily, at 29,411 to 30,274
GFF2183
GFF2184 - Predicted P-loop ATPase fused to an acetyltransferase COG1444, at 30,290 to 32,308
GFF2184
GFF2185 - Putative inner membrane protein, at 32,335 to 32,535
GFF2185
GFF2186 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 32,563 to 33,690
GFF2186
Position (kb) 30
31
32
33 Strain fitness (log2 ratio) -1
0
1 at 29.344 kb on + strand at 29.344 kb on + strand at 29.385 kb on + strand at 29.386 kb on - strand at 29.446 kb on - strand at 29.555 kb on + strand, within GFF2183 at 29.558 kb on + strand, within GFF2183 at 29.559 kb on - strand, within GFF2183 at 29.559 kb on - strand, within GFF2183 at 29.829 kb on - strand, within GFF2183 at 29.829 kb on - strand, within GFF2183 at 29.897 kb on + strand, within GFF2183 at 29.898 kb on - strand, within GFF2183 at 29.898 kb on - strand, within GFF2183 at 29.898 kb on - strand, within GFF2183 at 29.928 kb on + strand, within GFF2183 at 30.021 kb on + strand, within GFF2183 at 30.676 kb on + strand, within GFF2184 at 31.134 kb on + strand, within GFF2184 at 31.137 kb on + strand, within GFF2184 at 31.397 kb on + strand, within GFF2184 at 31.397 kb on + strand, within GFF2184 at 31.426 kb on + strand, within GFF2184 at 31.426 kb on + strand, within GFF2184 at 31.563 kb on + strand, within GFF2184 at 31.563 kb on + strand, within GFF2184 at 31.563 kb on + strand, within GFF2184 at 32.099 kb on + strand, within GFF2184 at 32.191 kb on + strand at 32.540 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 29,344 + -0.9 29,344 + -0.3 29,385 + +0.0 29,386 - -0.0 29,446 - +0.2 29,555 + GFF2183 0.17 +0.1 29,558 + GFF2183 0.17 +0.1 29,559 - GFF2183 0.17 +0.5 29,559 - GFF2183 0.17 +0.4 29,829 - GFF2183 0.48 -1.1 29,829 - GFF2183 0.48 -0.6 29,897 + GFF2183 0.56 +0.2 29,898 - GFF2183 0.56 -0.1 29,898 - GFF2183 0.56 -0.8 29,898 - GFF2183 0.56 +0.4 29,928 + GFF2183 0.60 +0.3 30,021 + GFF2183 0.71 +0.1 30,676 + GFF2184 0.19 +1.1 31,134 + GFF2184 0.42 +0.7 31,137 + GFF2184 0.42 +1.2 31,397 + GFF2184 0.55 +1.0 31,397 + GFF2184 0.55 +0.9 31,426 + GFF2184 0.56 +1.3 31,426 + GFF2184 0.56 +0.9 31,563 + GFF2184 0.63 +0.9 31,563 + GFF2184 0.63 +0.8 31,563 + GFF2184 0.63 -0.6 32,099 + GFF2184 0.90 +0.4 32,191 + +0.2 32,540 - +0.0
Or see this region's nucleotide sequence