Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2101

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2100 and GFF2101 are separated by 318 nucleotidesGFF2101 and GFF2102 are separated by 61 nucleotidesGFF2102 and GFF2103 are separated by 102 nucleotides GFF2100 - Uracil-DNA glycosylase, family 1, at 9,387 to 10,076 GFF2100 GFF2101 - Pyruvate formate-lyase (EC 2.3.1.54), at 10,395 to 10,778 GFF2101 GFF2102 - Transporter, LysE family, at 10,840 to 11,427 GFF2102 GFF2103 - LysR family transcriptional regulator YfiE, at 11,530 to 12,429 GFF2103 Position (kb) 10 11Strain fitness (log2 ratio) -2 -1 0 1at 9.533 kb on + strand, within GFF2100at 9.533 kb on + strand, within GFF2100at 9.799 kb on + strand, within GFF2100at 9.800 kb on - strand, within GFF2100at 9.804 kb on - strand, within GFF2100at 9.804 kb on - strand, within GFF2100at 9.804 kb on - strand, within GFF2100at 9.804 kb on - strand, within GFF2100at 10.010 kb on + strandat 10.106 kb on + strandat 10.309 kb on + strandat 10.309 kb on + strandat 10.309 kb on + strandat 10.309 kb on + strandat 10.310 kb on - strandat 10.311 kb on + strandat 10.312 kb on - strandat 10.372 kb on - strandat 10.406 kb on + strandat 10.415 kb on + strandat 10.418 kb on + strandat 10.890 kb on + strandat 10.894 kb on + strandat 10.987 kb on + strand, within GFF2102at 10.987 kb on + strand, within GFF2102at 10.987 kb on + strand, within GFF2102at 10.988 kb on - strand, within GFF2102at 10.990 kb on - strand, within GFF2102at 11.040 kb on + strand, within GFF2102at 11.040 kb on + strand, within GFF2102at 11.183 kb on + strand, within GFF2102at 11.183 kb on + strand, within GFF2102at 11.391 kb on + strandat 11.392 kb on - strandat 11.392 kb on - strandat 11.392 kb on - strandat 11.392 kb on - strandat 11.411 kb on - strandat 11.564 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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9,533 + GFF2100 0.21 +0.3
9,533 + GFF2100 0.21 +0.6
9,799 + GFF2100 0.60 +0.1
9,800 - GFF2100 0.60 -0.0
9,804 - GFF2100 0.60 -0.5
9,804 - GFF2100 0.60 -1.2
9,804 - GFF2100 0.60 +0.2
9,804 - GFF2100 0.60 -0.3
10,010 + +0.6
10,106 + +0.4
10,309 + +0.0
10,309 + +0.3
10,309 + -1.1
10,309 + +0.1
10,310 - +0.3
10,311 + -0.2
10,312 - -0.1
10,372 - +0.2
10,406 + -1.0
10,415 + -0.2
10,418 + +0.9
10,890 + -1.2
10,894 + +0.4
10,987 + GFF2102 0.25 +0.6
10,987 + GFF2102 0.25 +0.1
10,987 + GFF2102 0.25 -2.0
10,988 - GFF2102 0.25 +0.5
10,990 - GFF2102 0.26 +0.2
11,040 + GFF2102 0.34 -0.7
11,040 + GFF2102 0.34 -0.2
11,183 + GFF2102 0.58 -0.1
11,183 + GFF2102 0.58 -1.2
11,391 + +0.9
11,392 - -0.1
11,392 - -0.6
11,392 - -0.3
11,392 - +0.3
11,411 - +0.0
11,564 + +0.2

Or see this region's nucleotide sequence