Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2059 and GFF2060 are separated by 157 nucleotides GFF2060 and GFF2061 are separated by 237 nucleotides
GFF2059 - Sulfate and thiosulfate binding protein CysP, at 23,444 to 24,460
GFF2059
GFF2060 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 24,618 to 25,409
GFF2060
GFF2061 - Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup, at 25,647 to 26,546
GFF2061
Position (kb)
24
25
26 Strain fitness (log2 ratio)
-2
-1
0
1 at 23.779 kb on - strand, within GFF2059 at 23.779 kb on - strand, within GFF2059 at 23.815 kb on + strand, within GFF2059 at 23.976 kb on + strand, within GFF2059 at 24.201 kb on + strand, within GFF2059 at 24.274 kb on + strand, within GFF2059 at 24.275 kb on - strand, within GFF2059 at 24.364 kb on - strand at 24.503 kb on - strand at 24.506 kb on + strand at 24.688 kb on + strand at 24.947 kb on - strand, within GFF2060 at 24.974 kb on + strand, within GFF2060 at 24.975 kb on - strand, within GFF2060 at 25.068 kb on - strand, within GFF2060 at 25.068 kb on - strand, within GFF2060 at 25.068 kb on - strand, within GFF2060 at 25.070 kb on + strand, within GFF2060 at 25.076 kb on + strand, within GFF2060 at 25.076 kb on + strand, within GFF2060 at 25.166 kb on + strand, within GFF2060 at 25.311 kb on + strand, within GFF2060 at 25.555 kb on + strand at 25.555 kb on + strand at 25.648 kb on + strand at 25.684 kb on + strand at 25.685 kb on - strand at 25.754 kb on + strand, within GFF2061 at 25.796 kb on - strand, within GFF2061 at 25.808 kb on - strand, within GFF2061 at 25.808 kb on - strand, within GFF2061 at 26.038 kb on + strand, within GFF2061 at 26.038 kb on + strand, within GFF2061 at 26.039 kb on - strand, within GFF2061 at 26.039 kb on - strand, within GFF2061 at 26.272 kb on + strand, within GFF2061 at 26.272 kb on + strand, within GFF2061 at 26.303 kb on - strand, within GFF2061
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 23,779 - GFF2059 0.33 +0.2 23,779 - GFF2059 0.33 +0.2 23,815 + GFF2059 0.36 -0.3 23,976 + GFF2059 0.52 -1.0 24,201 + GFF2059 0.74 +0.2 24,274 + GFF2059 0.82 +0.1 24,275 - GFF2059 0.82 -0.1 24,364 - -0.2 24,503 - -0.1 24,506 + +0.0 24,688 + -1.1 24,947 - GFF2060 0.42 +0.2 24,974 + GFF2060 0.45 +0.2 24,975 - GFF2060 0.45 -0.3 25,068 - GFF2060 0.57 -0.2 25,068 - GFF2060 0.57 -0.2 25,068 - GFF2060 0.57 +0.2 25,070 + GFF2060 0.57 +0.6 25,076 + GFF2060 0.58 -0.1 25,076 + GFF2060 0.58 -0.5 25,166 + GFF2060 0.69 +0.2 25,311 + GFF2060 0.88 -0.1 25,555 + -0.8 25,555 + -0.2 25,648 + +0.4 25,684 + -0.4 25,685 - -1.0 25,754 + GFF2061 0.12 +0.3 25,796 - GFF2061 0.17 +0.5 25,808 - GFF2061 0.18 -1.7 25,808 - GFF2061 0.18 -0.1 26,038 + GFF2061 0.43 +0.4 26,038 + GFF2061 0.43 +1.4 26,039 - GFF2061 0.44 -1.8 26,039 - GFF2061 0.44 -0.0 26,272 + GFF2061 0.69 +0.6 26,272 + GFF2061 0.69 +0.7 26,303 - GFF2061 0.73 -0.1
Or see this region's nucleotide sequence