Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2033

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2086 and GFF2087 are separated by 4 nucleotidesGFF2087 and GFF2033 are separated by 163 nucleotidesGFF2033 and GFF2034 are separated by 51 nucleotidesGFF2034 and GFF2035 are separated by 58 nucleotides GFF2086 - tRNA-Val-TAC, at 94 to 169 GFF2086 GFF2087 - tRNA-Lys-TTT, at 174 to 249 GFF2087 GFF2033 - Xanthosine operon regulatory protein XapR, LysR family, at 413 to 1,297 GFF2033 GFF2034 - FIG00638837: hypothetical protein, at 1,349 to 1,507 GFF2034 GFF2035 - Xanthosine permease, at 1,566 to 2,822 GFF2035 Position (kb) 0 1 2Strain fitness (log2 ratio) -1 0 1at 0.391 kb on + strandat 0.415 kb on - strandat 0.415 kb on - strandat 0.478 kb on + strandat 0.481 kb on - strandat 0.496 kb on + strandat 0.547 kb on - strand, within GFF2033at 0.803 kb on + strand, within GFF2033at 0.804 kb on - strand, within GFF2033at 0.804 kb on - strand, within GFF2033at 0.811 kb on + strand, within GFF2033at 0.812 kb on - strand, within GFF2033at 0.838 kb on + strand, within GFF2033at 0.839 kb on - strand, within GFF2033at 1.148 kb on - strand, within GFF2033at 1.201 kb on + strand, within GFF2033at 1.677 kb on - strandat 1.803 kb on - strand, within GFF2035at 1.830 kb on + strand, within GFF2035at 1.961 kb on + strand, within GFF2035at 1.997 kb on + strand, within GFF2035at 2.144 kb on + strand, within GFF2035

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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391 + -0.2
415 - -0.0
415 - -0.3
478 + -0.5
481 - +0.5
496 + +0.4
547 - GFF2033 0.15 -0.1
803 + GFF2033 0.44 -0.2
804 - GFF2033 0.44 -0.2
804 - GFF2033 0.44 -0.4
811 + GFF2033 0.45 -0.3
812 - GFF2033 0.45 +0.3
838 + GFF2033 0.48 +0.6
839 - GFF2033 0.48 -0.8
1,148 - GFF2033 0.83 -0.9
1,201 + GFF2033 0.89 +0.3
1,677 - +0.2
1,803 - GFF2035 0.19 +0.2
1,830 + GFF2035 0.21 +0.3
1,961 + GFF2035 0.31 +0.2
1,997 + GFF2035 0.34 -0.7
2,144 + GFF2035 0.46 +0.4

Or see this region's nucleotide sequence