Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1978 and GFF1979 are separated by 52 nucleotides GFF1979 and GFF1984 are separated by 190 nucleotides GFF1984 and GFF1985 are separated by 42 nucleotides GFF1985 and GFF1986 are separated by 42 nucleotides GFF1986 and GFF1987 are separated by 42 nucleotides GFF1987 and GFF1980 are separated by 236 nucleotides
GFF1978 - Nucleoside permease NupC, at 171,693 to 172,895
GFF1978
GFF1979 - FIG00638309: hypothetical protein, at 172,948 to 175,137
GFF1979
GFF1984 - tRNA-Ala-GGC, at 175,328 to 175,403
GFF1984
GFF1985 - tRNA-Ala-GGC, at 175,446 to 175,521
GFF1985
GFF1986 - tRNA-Ala-GGC, at 175,564 to 175,639
GFF1986
GFF1987 - tRNA-Ala-GGC, at 175,682 to 175,757
GFF1987
GFF1980 - Putative negative regulator, at 175,994 to 176,341
GFF1980
Position (kb)
172
173
174
175
176 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 172.191 kb on + strand, within GFF1978 at 172.191 kb on + strand, within GFF1978 at 172.192 kb on - strand, within GFF1978 at 172.247 kb on + strand, within GFF1978 at 172.248 kb on - strand, within GFF1978 at 172.269 kb on + strand, within GFF1978 at 172.270 kb on - strand, within GFF1978 at 172.270 kb on - strand, within GFF1978 at 172.335 kb on + strand, within GFF1978 at 172.335 kb on + strand, within GFF1978 at 172.594 kb on + strand, within GFF1978 at 172.595 kb on - strand, within GFF1978 at 172.625 kb on + strand, within GFF1978 at 172.626 kb on - strand, within GFF1978 at 172.626 kb on - strand, within GFF1978 at 172.718 kb on + strand, within GFF1978 at 172.862 kb on - strand at 172.894 kb on - strand at 172.983 kb on - strand at 173.015 kb on - strand at 173.244 kb on + strand, within GFF1979 at 173.358 kb on + strand, within GFF1979 at 173.503 kb on - strand, within GFF1979 at 173.582 kb on + strand, within GFF1979 at 173.764 kb on + strand, within GFF1979 at 173.871 kb on + strand, within GFF1979 at 173.891 kb on + strand, within GFF1979 at 173.891 kb on + strand, within GFF1979 at 173.891 kb on + strand, within GFF1979 at 173.892 kb on - strand, within GFF1979 at 173.892 kb on - strand, within GFF1979 at 173.892 kb on - strand, within GFF1979 at 173.936 kb on - strand, within GFF1979 at 174.073 kb on + strand, within GFF1979 at 174.074 kb on - strand, within GFF1979 at 174.258 kb on + strand, within GFF1979 at 174.308 kb on + strand, within GFF1979 at 174.312 kb on + strand, within GFF1979 at 174.313 kb on - strand, within GFF1979 at 174.426 kb on - strand, within GFF1979 at 174.546 kb on + strand, within GFF1979 at 174.552 kb on + strand, within GFF1979 at 174.569 kb on - strand, within GFF1979 at 174.701 kb on + strand, within GFF1979 at 174.790 kb on + strand, within GFF1979 at 174.827 kb on - strand, within GFF1979 at 175.014 kb on + strand at 175.456 kb on - strand, within GFF1985 at 175.456 kb on - strand, within GFF1985 at 175.979 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 172,191 + GFF1978 0.41 +0.2 172,191 + GFF1978 0.41 +1.4 172,192 - GFF1978 0.41 +0.9 172,247 + GFF1978 0.46 +0.5 172,248 - GFF1978 0.46 +1.3 172,269 + GFF1978 0.48 +1.1 172,270 - GFF1978 0.48 +1.1 172,270 - GFF1978 0.48 +1.4 172,335 + GFF1978 0.53 +1.2 172,335 + GFF1978 0.53 +0.3 172,594 + GFF1978 0.75 +1.2 172,595 - GFF1978 0.75 +0.3 172,625 + GFF1978 0.77 +1.0 172,626 - GFF1978 0.78 +1.2 172,626 - GFF1978 0.78 +0.5 172,718 + GFF1978 0.85 +1.0 172,862 - +1.1 172,894 - -0.5 172,983 - -0.0 173,015 - -0.1 173,244 + GFF1979 0.14 -1.1 173,358 + GFF1979 0.19 +1.5 173,503 - GFF1979 0.25 -2.0 173,582 + GFF1979 0.29 +0.2 173,764 + GFF1979 0.37 +0.1 173,871 + GFF1979 0.42 +0.1 173,891 + GFF1979 0.43 -0.3 173,891 + GFF1979 0.43 +0.1 173,891 + GFF1979 0.43 -0.9 173,892 - GFF1979 0.43 +0.4 173,892 - GFF1979 0.43 +0.1 173,892 - GFF1979 0.43 +0.6 173,936 - GFF1979 0.45 -0.2 174,073 + GFF1979 0.51 -0.1 174,074 - GFF1979 0.51 +0.1 174,258 + GFF1979 0.60 -0.3 174,308 + GFF1979 0.62 +1.1 174,312 + GFF1979 0.62 +0.1 174,313 - GFF1979 0.62 -0.3 174,426 - GFF1979 0.67 +0.5 174,546 + GFF1979 0.73 -1.0 174,552 + GFF1979 0.73 -0.5 174,569 - GFF1979 0.74 +0.2 174,701 + GFF1979 0.80 +1.4 174,790 + GFF1979 0.84 +0.8 174,827 - GFF1979 0.86 +0.3 175,014 + +0.2 175,456 - GFF1985 0.13 +0.2 175,456 - GFF1985 0.13 +1.0 175,979 - -2.8
Or see this region's nucleotide sequence