Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1933 and GFF1934 are separated by 48 nucleotides GFF1934 and GFF1935 overlap by 1 nucleotides GFF1935 and GFF1936 are separated by 67 nucleotides
GFF1933 - DedA protein, at 126,144 to 126,803
GFF1933
GFF1934 - tRNA pseudouridine synthase A (EC 4.2.1.70), at 126,852 to 127,664
GFF1934
GFF1935 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 127,664 to 128,677
GFF1935
GFF1936 - Erythronate-4-phosphate dehydrogenase (EC 1.1.1.290), at 128,745 to 129,881
GFF1936
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 126.786 kb on - strand at 126.853 kb on + strand at 127.047 kb on - strand, within GFF1934 at 127.086 kb on - strand, within GFF1934 at 127.099 kb on + strand, within GFF1934 at 127.247 kb on - strand, within GFF1934 at 127.273 kb on - strand, within GFF1934 at 127.298 kb on - strand, within GFF1934 at 127.305 kb on - strand, within GFF1934 at 127.357 kb on - strand, within GFF1934 at 127.514 kb on + strand, within GFF1934 at 127.515 kb on - strand, within GFF1934 at 127.598 kb on - strand at 127.675 kb on - strand at 127.675 kb on - strand at 127.799 kb on - strand, within GFF1935 at 127.868 kb on - strand, within GFF1935 at 127.953 kb on + strand, within GFF1935 at 128.032 kb on + strand, within GFF1935 at 128.084 kb on - strand, within GFF1935 at 128.206 kb on + strand, within GFF1935 at 128.306 kb on - strand, within GFF1935 at 128.397 kb on - strand, within GFF1935 at 128.697 kb on - strand at 128.700 kb on + strand at 128.700 kb on + strand at 128.900 kb on + strand, within GFF1936 at 128.933 kb on - strand, within GFF1936 at 128.987 kb on + strand, within GFF1936 at 128.987 kb on + strand, within GFF1936 at 128.988 kb on - strand, within GFF1936 at 129.015 kb on + strand, within GFF1936 at 129.061 kb on + strand, within GFF1936 at 129.108 kb on - strand, within GFF1936 at 129.162 kb on - strand, within GFF1936 at 129.334 kb on + strand, within GFF1936 at 129.350 kb on - strand, within GFF1936 at 129.525 kb on + strand, within GFF1936 at 129.528 kb on - strand, within GFF1936 at 129.589 kb on + strand, within GFF1936 at 129.605 kb on + strand, within GFF1936 at 129.611 kb on + strand, within GFF1936 at 129.611 kb on + strand, within GFF1936 at 129.611 kb on + strand, within GFF1936 at 129.612 kb on - strand, within GFF1936
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 126,786 - -2.5 126,853 + -2.8 127,047 - GFF1934 0.24 +0.5 127,086 - GFF1934 0.29 +1.6 127,099 + GFF1934 0.30 +0.5 127,247 - GFF1934 0.49 +1.1 127,273 - GFF1934 0.52 +1.7 127,298 - GFF1934 0.55 +0.5 127,305 - GFF1934 0.56 +1.6 127,357 - GFF1934 0.62 +1.0 127,514 + GFF1934 0.81 +0.1 127,515 - GFF1934 0.82 +1.0 127,598 - -0.5 127,675 - -1.2 127,675 - +0.1 127,799 - GFF1935 0.13 +0.1 127,868 - GFF1935 0.20 +2.1 127,953 + GFF1935 0.29 +0.4 128,032 + GFF1935 0.36 +0.5 128,084 - GFF1935 0.41 +1.0 128,206 + GFF1935 0.53 +0.3 128,306 - GFF1935 0.63 +2.5 128,397 - GFF1935 0.72 +0.3 128,697 - -0.5 128,700 + +1.1 128,700 + +0.7 128,900 + GFF1936 0.14 -2.0 128,933 - GFF1936 0.17 -3.9 128,987 + GFF1936 0.21 -3.6 128,987 + GFF1936 0.21 -3.1 128,988 - GFF1936 0.21 -2.5 129,015 + GFF1936 0.24 -2.7 129,061 + GFF1936 0.28 -3.1 129,108 - GFF1936 0.32 -2.9 129,162 - GFF1936 0.37 -3.9 129,334 + GFF1936 0.52 -2.2 129,350 - GFF1936 0.53 -4.5 129,525 + GFF1936 0.69 -0.9 129,528 - GFF1936 0.69 -2.4 129,589 + GFF1936 0.74 -3.3 129,605 + GFF1936 0.76 -3.0 129,611 + GFF1936 0.76 -4.0 129,611 + GFF1936 0.76 -4.0 129,611 + GFF1936 0.76 -2.2 129,612 - GFF1936 0.76 -2.3
Or see this region's nucleotide sequence