Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1800 and GFF1801 are separated by 86 nucleotides GFF1801 and GFF1802 are separated by 264 nucleotides GFF1802 and GFF1803 are separated by 158 nucleotides
GFF1800 - Transcriptional regulatory protein RstA, at 21,764 to 22,495
GFF1800
GFF1801 - hypothetical protein, at 22,582 to 22,710
GFF1801
GFF1802 - Outer membrane protein N precursor, at 22,975 to 24,108
GFF1802
GFF1803 - Exoenzymes regulatory protein AepA precursor, at 24,267 to 25,967
GFF1803
Position (kb) 22
23
24
25 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 22.304 kb on - strand, within GFF1800 at 22.347 kb on - strand, within GFF1800 at 22.430 kb on - strand at 22.494 kb on - strand at 22.513 kb on - strand at 22.533 kb on - strand at 22.580 kb on - strand at 22.596 kb on + strand, within GFF1801 at 23.142 kb on + strand, within GFF1802 at 23.230 kb on - strand, within GFF1802 at 23.316 kb on + strand, within GFF1802 at 23.383 kb on + strand, within GFF1802 at 23.383 kb on + strand, within GFF1802 at 23.389 kb on + strand, within GFF1802 at 23.600 kb on - strand, within GFF1802 at 23.671 kb on + strand, within GFF1802 at 23.692 kb on - strand, within GFF1802 at 23.730 kb on + strand, within GFF1802 at 23.731 kb on - strand, within GFF1802 at 23.772 kb on + strand, within GFF1802 at 23.896 kb on - strand, within GFF1802 at 23.974 kb on + strand, within GFF1802 at 24.265 kb on + strand at 24.384 kb on - strand at 24.500 kb on + strand, within GFF1803 at 24.512 kb on - strand, within GFF1803 at 24.518 kb on + strand, within GFF1803 at 24.773 kb on + strand, within GFF1803 at 24.773 kb on + strand, within GFF1803 at 24.773 kb on + strand, within GFF1803 at 24.774 kb on - strand, within GFF1803 at 24.774 kb on - strand, within GFF1803 at 24.787 kb on - strand, within GFF1803 at 24.793 kb on - strand, within GFF1803 at 24.817 kb on + strand, within GFF1803 at 24.824 kb on - strand, within GFF1803 at 24.824 kb on - strand, within GFF1803 at 25.066 kb on + strand, within GFF1803
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 22,304 - GFF1800 0.74 +0.2 22,347 - GFF1800 0.80 -0.3 22,430 - -0.9 22,494 - +0.7 22,513 - +1.4 22,533 - -1.5 22,580 - +0.6 22,596 + GFF1801 0.11 -1.1 23,142 + GFF1802 0.15 +0.9 23,230 - GFF1802 0.22 +0.3 23,316 + GFF1802 0.30 +0.8 23,383 + GFF1802 0.36 +0.1 23,383 + GFF1802 0.36 -0.9 23,389 + GFF1802 0.37 +0.2 23,600 - GFF1802 0.55 +0.7 23,671 + GFF1802 0.61 -1.2 23,692 - GFF1802 0.63 +1.0 23,730 + GFF1802 0.67 -2.8 23,731 - GFF1802 0.67 +0.5 23,772 + GFF1802 0.70 -0.1 23,896 - GFF1802 0.81 +0.7 23,974 + GFF1802 0.88 +0.6 24,265 + +0.2 24,384 - -0.1 24,500 + GFF1803 0.14 +1.0 24,512 - GFF1803 0.14 +0.1 24,518 + GFF1803 0.15 +0.4 24,773 + GFF1803 0.30 +0.9 24,773 + GFF1803 0.30 +1.2 24,773 + GFF1803 0.30 -1.5 24,774 - GFF1803 0.30 -0.2 24,774 - GFF1803 0.30 +0.3 24,787 - GFF1803 0.31 -0.7 24,793 - GFF1803 0.31 -0.6 24,817 + GFF1803 0.32 +0.0 24,824 - GFF1803 0.33 +0.0 24,824 - GFF1803 0.33 +0.1 25,066 + GFF1803 0.47 -0.3
Or see this region's nucleotide sequence