Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1787 and GFF1788 are separated by 394 nucleotides GFF1788 and GFF1789 are separated by 49 nucleotides GFF1789 and GFF1790 are separated by 99 nucleotides GFF1790 and GFF1791 are separated by 39 nucleotides GFF1791 and GFF1792 overlap by 14 nucleotides GFF1792 and GFF1793 are separated by 111 nucleotides
GFF1787 - Permeases of the major facilitator superfamily, at 9,829 to 11,082
GFF1787
GFF1788 - Acid shock protein precursor, at 11,477 to 11,725
GFF1788
GFF1789 - hypothetical protein, at 11,775 to 11,894
GFF1789
GFF1790 - FIG00634494: possible peptidase, at 11,994 to 12,815
GFF1790
GFF1791 - Spermidine export protein MdtI, at 12,855 to 13,184
GFF1791
GFF1792 - Spermidine export protein MdtJ, at 13,171 to 13,533
GFF1792
GFF1793 - FIG01046102: hypothetical protein, at 13,645 to 13,782
GFF1793
Position (kb) 11
12
13 Strain fitness (log2 ratio) -2
-1
0
1 at 11.025 kb on - strand at 11.060 kb on + strand at 11.215 kb on - strand at 11.242 kb on - strand at 11.318 kb on + strand at 11.322 kb on + strand at 11.326 kb on - strand at 11.362 kb on - strand at 11.371 kb on + strand at 11.371 kb on + strand at 11.419 kb on + strand at 11.707 kb on + strand at 11.999 kb on + strand at 12.000 kb on - strand at 12.026 kb on - strand at 12.110 kb on + strand, within GFF1790 at 12.175 kb on + strand, within GFF1790 at 12.254 kb on - strand, within GFF1790 at 12.481 kb on + strand, within GFF1790 at 12.677 kb on - strand, within GFF1790 at 12.713 kb on - strand, within GFF1790 at 13.017 kb on + strand, within GFF1791 at 13.018 kb on - strand, within GFF1791 at 13.066 kb on - strand, within GFF1791 at 13.082 kb on + strand, within GFF1791 at 13.083 kb on - strand, within GFF1791 at 13.164 kb on + strand at 13.237 kb on + strand, within GFF1792 at 13.401 kb on + strand, within GFF1792 at 13.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 11,025 - +0.4 11,060 + +0.0 11,215 - +0.2 11,242 - -0.2 11,318 + +0.2 11,322 + +0.0 11,326 - +1.0 11,362 - -0.1 11,371 + -1.6 11,371 + -0.9 11,419 + +0.0 11,707 + -0.1 11,999 + -0.5 12,000 - -0.0 12,026 - +0.4 12,110 + GFF1790 0.14 +0.0 12,175 + GFF1790 0.22 -0.4 12,254 - GFF1790 0.32 +0.3 12,481 + GFF1790 0.59 +0.5 12,677 - GFF1790 0.83 -0.5 12,713 - GFF1790 0.87 -2.3 13,017 + GFF1791 0.49 +0.5 13,018 - GFF1791 0.49 +0.3 13,066 - GFF1791 0.64 -1.0 13,082 + GFF1791 0.69 +0.6 13,083 - GFF1791 0.69 +0.0 13,164 + +0.9 13,237 + GFF1792 0.18 +0.5 13,401 + GFF1792 0.63 +1.0 13,656 + +0.1
Or see this region's nucleotide sequence