Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1734 and GFF1735 are separated by 135 nucleotides GFF1735 and GFF1736 overlap by 1 nucleotides GFF1736 and GFF1737 are separated by 50 nucleotides
GFF1734 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 35,214 to 36,254
GFF1734
GFF1735 - Phosphate transport system permease protein PstC (TC 3.A.1.7.1), at 36,390 to 37,349
GFF1735
GFF1736 - Phosphate transport system permease protein PstA (TC 3.A.1.7.1), at 37,349 to 38,239
GFF1736
GFF1737 - Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1), at 38,290 to 39,099
GFF1737
Position (kb) 36
37
38 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 35.730 kb on + strand, within GFF1734 at 35.780 kb on + strand, within GFF1734 at 35.839 kb on + strand, within GFF1734 at 36.059 kb on + strand, within GFF1734 at 36.129 kb on + strand, within GFF1734 at 36.165 kb on + strand at 36.481 kb on + strand at 36.566 kb on + strand, within GFF1735 at 36.594 kb on + strand, within GFF1735 at 36.788 kb on + strand, within GFF1735 at 36.939 kb on + strand, within GFF1735 at 36.939 kb on + strand, within GFF1735 at 36.939 kb on + strand, within GFF1735 at 37.016 kb on + strand, within GFF1735 at 37.019 kb on + strand, within GFF1735 at 37.082 kb on + strand, within GFF1735 at 37.146 kb on + strand, within GFF1735 at 37.146 kb on + strand, within GFF1735 at 37.170 kb on + strand, within GFF1735 at 37.170 kb on + strand, within GFF1735 at 37.316 kb on + strand at 37.632 kb on + strand, within GFF1736 at 37.667 kb on + strand, within GFF1736 at 38.184 kb on + strand at 38.248 kb on + strand at 38.248 kb on + strand at 38.257 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 35,730 + GFF1734 0.50 -0.6 35,780 + GFF1734 0.54 -0.5 35,839 + GFF1734 0.60 -1.1 36,059 + GFF1734 0.81 -1.4 36,129 + GFF1734 0.88 -0.4 36,165 + -1.2 36,481 + -3.9 36,566 + GFF1735 0.18 -3.1 36,594 + GFF1735 0.21 -1.2 36,788 + GFF1735 0.41 -4.1 36,939 + GFF1735 0.57 -2.5 36,939 + GFF1735 0.57 -3.1 36,939 + GFF1735 0.57 -3.8 37,016 + GFF1735 0.65 -1.8 37,019 + GFF1735 0.66 -1.9 37,082 + GFF1735 0.72 -2.1 37,146 + GFF1735 0.79 +0.1 37,146 + GFF1735 0.79 -0.7 37,170 + GFF1735 0.81 -1.1 37,170 + GFF1735 0.81 -1.2 37,316 + -0.9 37,632 + GFF1736 0.32 -0.1 37,667 + GFF1736 0.36 -1.9 38,184 + -0.3 38,248 + +0.1 38,248 + -1.1 38,257 + -0.2
Or see this region's nucleotide sequence