Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1702 and GFF1703 are separated by 11 nucleotides GFF1703 and GFF1704 overlap by 35 nucleotides GFF1704 and GFF1705 are separated by 12 nucleotides
GFF1702 - Transcriptional regulator, GntR family, at 188 to 892
GFF1702
GFF1703 - Permeases of the major facilitator superfamily, at 904 to 2,331
GFF1703
GFF1704 - Ribose operon repressor, at 2,297 to 3,286
GFF1704
GFF1705 - Ribokinase (EC 2.7.1.15), at 3,299 to 4,228
GFF1705
Position (kb)
0
1
2
3 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 0.099 kb on + strand at 0.151 kb on + strand at 0.154 kb on + strand at 0.154 kb on + strand at 0.154 kb on + strand at 0.234 kb on - strand at 0.267 kb on + strand, within GFF1702 at 0.267 kb on + strand, within GFF1702 at 0.268 kb on - strand, within GFF1702 at 0.278 kb on + strand, within GFF1702 at 0.326 kb on + strand, within GFF1702 at 0.382 kb on + strand, within GFF1702 at 0.382 kb on + strand, within GFF1702 at 0.391 kb on - strand, within GFF1702 at 0.520 kb on + strand, within GFF1702 at 0.521 kb on - strand, within GFF1702 at 0.676 kb on + strand, within GFF1702 at 0.677 kb on - strand, within GFF1702 at 0.677 kb on - strand, within GFF1702 at 0.677 kb on - strand, within GFF1702 at 0.680 kb on + strand, within GFF1702 at 0.723 kb on + strand, within GFF1702 at 0.746 kb on + strand, within GFF1702 at 0.747 kb on - strand, within GFF1702 at 0.747 kb on - strand, within GFF1702 at 0.764 kb on + strand, within GFF1702 at 0.777 kb on + strand, within GFF1702 at 0.864 kb on + strand at 0.886 kb on + strand at 1.059 kb on + strand, within GFF1703 at 1.060 kb on - strand, within GFF1703 at 1.067 kb on - strand, within GFF1703 at 1.083 kb on + strand, within GFF1703 at 1.083 kb on + strand, within GFF1703 at 1.084 kb on - strand, within GFF1703 at 1.088 kb on + strand, within GFF1703 at 1.152 kb on - strand, within GFF1703 at 1.162 kb on - strand, within GFF1703 at 1.226 kb on + strand, within GFF1703 at 1.226 kb on + strand, within GFF1703 at 1.226 kb on + strand, within GFF1703 at 1.226 kb on + strand, within GFF1703 at 1.227 kb on - strand, within GFF1703 at 1.227 kb on - strand, within GFF1703 at 1.274 kb on + strand, within GFF1703 at 1.340 kb on + strand, within GFF1703 at 1.374 kb on - strand, within GFF1703 at 1.414 kb on - strand, within GFF1703 at 1.416 kb on + strand, within GFF1703 at 1.444 kb on + strand, within GFF1703 at 1.445 kb on - strand, within GFF1703 at 1.445 kb on - strand, within GFF1703 at 1.445 kb on - strand, within GFF1703 at 1.445 kb on - strand, within GFF1703 at 1.457 kb on - strand, within GFF1703 at 1.457 kb on - strand, within GFF1703 at 1.457 kb on - strand, within GFF1703 at 1.457 kb on - strand, within GFF1703 at 1.459 kb on - strand, within GFF1703 at 1.609 kb on - strand, within GFF1703 at 1.619 kb on + strand, within GFF1703 at 1.664 kb on + strand, within GFF1703 at 1.665 kb on - strand, within GFF1703 at 1.836 kb on + strand, within GFF1703 at 1.837 kb on - strand, within GFF1703 at 1.837 kb on - strand, within GFF1703 at 1.837 kb on - strand, within GFF1703 at 1.856 kb on + strand, within GFF1703 at 1.868 kb on + strand, within GFF1703 at 1.868 kb on + strand, within GFF1703 at 1.915 kb on - strand, within GFF1703 at 1.915 kb on - strand, within GFF1703 at 1.917 kb on - strand, within GFF1703 at 1.986 kb on - strand, within GFF1703 at 2.031 kb on + strand, within GFF1703 at 2.148 kb on + strand, within GFF1703 at 2.148 kb on + strand, within GFF1703 at 2.148 kb on + strand, within GFF1703 at 2.153 kb on - strand, within GFF1703 at 2.195 kb on - strand at 2.195 kb on - strand at 2.195 kb on - strand at 2.196 kb on + strand at 2.196 kb on + strand at 2.197 kb on - strand at 2.197 kb on - strand at 2.197 kb on - strand at 2.244 kb on + strand at 2.252 kb on + strand at 2.253 kb on - strand at 2.293 kb on - strand at 2.484 kb on - strand, within GFF1704 at 2.520 kb on - strand, within GFF1704 at 2.650 kb on - strand, within GFF1704 at 2.650 kb on - strand, within GFF1704 at 2.744 kb on - strand, within GFF1704 at 2.744 kb on - strand, within GFF1704 at 2.778 kb on + strand, within GFF1704 at 2.812 kb on + strand, within GFF1704 at 2.813 kb on - strand, within GFF1704 at 2.878 kb on + strand, within GFF1704 at 3.025 kb on + strand, within GFF1704 at 3.075 kb on + strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.102 kb on - strand, within GFF1704 at 3.128 kb on + strand, within GFF1704 at 3.162 kb on + strand, within GFF1704 at 3.286 kb on + strand at 3.286 kb on + strand at 3.287 kb on - strand at 3.289 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 99 + -6.1 151 + -4.9 154 + -5.7 154 + -6.1 154 + -4.5 234 - +0.9 267 + GFF1702 0.11 +0.3 267 + GFF1702 0.11 +0.9 268 - GFF1702 0.11 +0.2 278 + GFF1702 0.13 +0.8 326 + GFF1702 0.20 +1.0 382 + GFF1702 0.28 +0.7 382 + GFF1702 0.28 +1.9 391 - GFF1702 0.29 +0.9 520 + GFF1702 0.47 +0.4 521 - GFF1702 0.47 +1.1 676 + GFF1702 0.69 +1.0 677 - GFF1702 0.69 +0.8 677 - GFF1702 0.69 +1.0 677 - GFF1702 0.69 +0.8 680 + GFF1702 0.70 +1.0 723 + GFF1702 0.76 +0.7 746 + GFF1702 0.79 +1.1 747 - GFF1702 0.79 +0.7 747 - GFF1702 0.79 +0.8 764 + GFF1702 0.82 +0.4 777 + GFF1702 0.84 +0.5 864 + -0.0 886 + +0.2 1,059 + GFF1703 0.11 +0.3 1,060 - GFF1703 0.11 -1.1 1,067 - GFF1703 0.11 +0.1 1,083 + GFF1703 0.13 -1.2 1,083 + GFF1703 0.13 -0.4 1,084 - GFF1703 0.13 +0.6 1,088 + GFF1703 0.13 -0.5 1,152 - GFF1703 0.17 +0.5 1,162 - GFF1703 0.18 -0.2 1,226 + GFF1703 0.23 +1.4 1,226 + GFF1703 0.23 +0.3 1,226 + GFF1703 0.23 +0.2 1,226 + GFF1703 0.23 -0.2 1,227 - GFF1703 0.23 -0.6 1,227 - GFF1703 0.23 +0.1 1,274 + GFF1703 0.26 +0.5 1,340 + GFF1703 0.31 +0.3 1,374 - GFF1703 0.33 +0.5 1,414 - GFF1703 0.36 +0.4 1,416 + GFF1703 0.36 -0.9 1,444 + GFF1703 0.38 +0.1 1,445 - GFF1703 0.38 -0.1 1,445 - GFF1703 0.38 +0.3 1,445 - GFF1703 0.38 +0.8 1,445 - GFF1703 0.38 +0.1 1,457 - GFF1703 0.39 +0.6 1,457 - GFF1703 0.39 -1.4 1,457 - GFF1703 0.39 +0.3 1,457 - GFF1703 0.39 -0.8 1,459 - GFF1703 0.39 +0.3 1,609 - GFF1703 0.49 -0.3 1,619 + GFF1703 0.50 -0.8 1,664 + GFF1703 0.53 +0.8 1,665 - GFF1703 0.53 +0.5 1,836 + GFF1703 0.65 +0.6 1,837 - GFF1703 0.65 +0.1 1,837 - GFF1703 0.65 -0.9 1,837 - GFF1703 0.65 -0.4 1,856 + GFF1703 0.67 +0.1 1,868 + GFF1703 0.68 +0.2 1,868 + GFF1703 0.68 +0.1 1,915 - GFF1703 0.71 +1.4 1,915 - GFF1703 0.71 +0.2 1,917 - GFF1703 0.71 -0.1 1,986 - GFF1703 0.76 +1.6 2,031 + GFF1703 0.79 -0.0 2,148 + GFF1703 0.87 +0.1 2,148 + GFF1703 0.87 +0.1 2,148 + GFF1703 0.87 +0.8 2,153 - GFF1703 0.87 -0.2 2,195 - -0.0 2,195 - -0.2 2,195 - -0.3 2,196 + +0.2 2,196 + +0.5 2,197 - +0.3 2,197 - -0.2 2,197 - +0.5 2,244 + -0.7 2,252 + -0.0 2,253 - -0.6 2,293 - +1.2 2,484 - GFF1704 0.19 -1.3 2,520 - GFF1704 0.23 -1.2 2,650 - GFF1704 0.36 -0.5 2,650 - GFF1704 0.36 -0.9 2,744 - GFF1704 0.45 +0.4 2,744 - GFF1704 0.45 -0.4 2,778 + GFF1704 0.49 +0.1 2,812 + GFF1704 0.52 -0.8 2,813 - GFF1704 0.52 -0.4 2,878 + GFF1704 0.59 -0.8 3,025 + GFF1704 0.74 -0.6 3,075 + GFF1704 0.79 -0.7 3,102 - GFF1704 0.81 -0.7 3,102 - GFF1704 0.81 -0.2 3,102 - GFF1704 0.81 -0.1 3,102 - GFF1704 0.81 -0.7 3,128 + GFF1704 0.84 -0.5 3,162 + GFF1704 0.87 -2.1 3,286 + -0.9 3,286 + +0.3 3,287 - -0.1 3,289 + -2.7
Or see this region's nucleotide sequence