Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1637 and GFF1638 overlap by 4 nucleotides GFF1638 and GFF1639 are separated by 78 nucleotides GFF1639 and GFF1640 are separated by 8 nucleotides GFF1640 and GFF1641 are separated by 174 nucleotides GFF1641 and GFF1642 are separated by 32 nucleotides
GFF1637 - Zinc ABC transporter, ATP-binding protein ZnuC, at 23,859 to 24,614
GFF1637
GFF1638 - Zinc ABC transporter, inner membrane permease protein ZnuB, at 24,611 to 25,396
GFF1638
GFF1639 - Holliday junction DNA helicase RuvB, at 25,475 to 26,485
GFF1639
GFF1640 - Holliday junction DNA helicase RuvA, at 26,494 to 27,105
GFF1640
GFF1641 - hypothetical protein, at 27,280 to 27,405
GFF1641
GFF1642 - putative cytoplasmic protein, at 27,438 to 28,421
GFF1642
Position (kb) 25
26
27 Strain fitness (log2 ratio) -1
0
1 at 24.503 kb on + strand, within GFF1637 at 24.826 kb on - strand, within GFF1638 at 24.875 kb on - strand, within GFF1638 at 24.891 kb on + strand, within GFF1638 at 24.950 kb on + strand, within GFF1638 at 24.950 kb on + strand, within GFF1638 at 24.992 kb on - strand, within GFF1638 at 25.003 kb on + strand, within GFF1638 at 25.016 kb on - strand, within GFF1638 at 25.066 kb on + strand, within GFF1638 at 25.122 kb on + strand, within GFF1638 at 25.148 kb on - strand, within GFF1638 at 25.273 kb on + strand, within GFF1638 at 25.273 kb on + strand, within GFF1638 at 25.274 kb on - strand, within GFF1638 at 25.314 kb on - strand, within GFF1638 at 25.373 kb on - strand at 27.019 kb on - strand, within GFF1640 at 27.150 kb on - strand at 27.196 kb on + strand at 27.390 kb on - strand, within GFF1641
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 24,503 + GFF1637 0.85 +0.1 24,826 - GFF1638 0.27 +0.4 24,875 - GFF1638 0.34 +0.8 24,891 + GFF1638 0.36 -0.1 24,950 + GFF1638 0.43 +0.1 24,950 + GFF1638 0.43 -0.3 24,992 - GFF1638 0.48 +0.1 25,003 + GFF1638 0.50 +0.3 25,016 - GFF1638 0.52 +0.1 25,066 + GFF1638 0.58 -0.6 25,122 + GFF1638 0.65 -1.4 25,148 - GFF1638 0.68 +0.6 25,273 + GFF1638 0.84 +0.5 25,273 + GFF1638 0.84 -0.9 25,274 - GFF1638 0.84 +0.6 25,314 - GFF1638 0.89 +0.3 25,373 - -0.1 27,019 - GFF1640 0.86 -0.6 27,150 - -0.2 27,196 + -0.2 27,390 - GFF1641 0.87 -0.8
Or see this region's nucleotide sequence