Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1585 and GFF1586 are separated by 10 nucleotides GFF1586 and GFF1587 are separated by 354 nucleotides
GFF1585 - Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1), at 40,664 to 42,808
GFF1585
GFF1586 - Ribonucleotide reductase of class Ib (aerobic), beta subunit (EC 1.17.4.1), at 42,819 to 43,778
GFF1586
GFF1587 - L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1), at 44,133 to 45,335
GFF1587
Position (kb) 42
43
44 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 41.880 kb on - strand, within GFF1585 at 41.967 kb on + strand, within GFF1585 at 42.013 kb on + strand, within GFF1585 at 42.014 kb on - strand, within GFF1585 at 42.016 kb on - strand, within GFF1585 at 42.110 kb on - strand, within GFF1585 at 42.166 kb on + strand, within GFF1585 at 42.166 kb on + strand, within GFF1585 at 42.184 kb on + strand, within GFF1585 at 42.185 kb on - strand, within GFF1585 at 42.319 kb on - strand, within GFF1585 at 42.398 kb on + strand, within GFF1585 at 42.398 kb on + strand, within GFF1585 at 42.398 kb on + strand, within GFF1585 at 42.399 kb on - strand, within GFF1585 at 42.448 kb on + strand, within GFF1585 at 42.541 kb on + strand, within GFF1585 at 42.567 kb on - strand, within GFF1585 at 42.607 kb on + strand at 42.608 kb on - strand at 42.707 kb on + strand at 42.725 kb on - strand at 42.728 kb on - strand at 42.728 kb on - strand at 42.740 kb on + strand at 42.806 kb on + strand at 42.819 kb on - strand at 42.932 kb on - strand, within GFF1586 at 43.008 kb on - strand, within GFF1586 at 43.132 kb on - strand, within GFF1586 at 43.139 kb on + strand, within GFF1586 at 43.289 kb on - strand, within GFF1586 at 43.507 kb on - strand, within GFF1586 at 43.537 kb on + strand, within GFF1586 at 43.584 kb on + strand, within GFF1586 at 43.595 kb on + strand, within GFF1586 at 43.729 kb on - strand at 44.265 kb on - strand, within GFF1587 at 44.300 kb on - strand, within GFF1587 at 44.405 kb on + strand, within GFF1587 at 44.492 kb on + strand, within GFF1587
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 41,880 - GFF1585 0.57 -0.2 41,967 + GFF1585 0.61 +0.2 42,013 + GFF1585 0.63 +0.3 42,014 - GFF1585 0.63 +0.7 42,016 - GFF1585 0.63 -0.4 42,110 - GFF1585 0.67 -0.1 42,166 + GFF1585 0.70 -3.2 42,166 + GFF1585 0.70 -0.2 42,184 + GFF1585 0.71 -0.1 42,185 - GFF1585 0.71 -0.1 42,319 - GFF1585 0.77 -0.1 42,398 + GFF1585 0.81 +0.3 42,398 + GFF1585 0.81 +0.2 42,398 + GFF1585 0.81 +0.4 42,399 - GFF1585 0.81 -0.3 42,448 + GFF1585 0.83 +0.5 42,541 + GFF1585 0.88 -0.5 42,567 - GFF1585 0.89 -0.2 42,607 + +0.2 42,608 - +0.1 42,707 + -0.3 42,725 - -0.1 42,728 - -0.4 42,728 - -0.3 42,740 + -1.0 42,806 + +0.3 42,819 - -0.1 42,932 - GFF1586 0.12 -0.8 43,008 - GFF1586 0.20 -0.3 43,132 - GFF1586 0.33 -0.4 43,139 + GFF1586 0.33 +0.3 43,289 - GFF1586 0.49 -0.4 43,507 - GFF1586 0.72 -1.2 43,537 + GFF1586 0.75 -0.2 43,584 + GFF1586 0.80 +0.0 43,595 + GFF1586 0.81 -0.8 43,729 - -0.4 44,265 - GFF1587 0.11 +0.5 44,300 - GFF1587 0.14 -0.2 44,405 + GFF1587 0.23 +0.1 44,492 + GFF1587 0.30 +0.7
Or see this region's nucleotide sequence