Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1505 and GFF1506 are separated by 60 nucleotides GFF1506 and GFF1507 are separated by 158 nucleotides GFF1507 and GFF1508 overlap by 23 nucleotides
GFF1505 - Tryptophan-specific transport protein, at 12,527 to 13,771
GFF1505
GFF1506 - FIG074102: hypothetical protein, at 13,832 to 14,362
GFF1506
GFF1507 - probable exported protein STY0357, at 14,521 to 15,240
GFF1507
GFF1508 - Luciferase-like monooxygenase (EC 1.14.-.-), at 15,218 to 16,225
GFF1508
Position (kb) 14
15
16 Strain fitness (log2 ratio) -2
-1
0
1
2 at 13.584 kb on + strand, within GFF1505 at 13.594 kb on + strand, within GFF1505 at 14.002 kb on - strand, within GFF1506 at 14.766 kb on + strand, within GFF1507 at 14.769 kb on - strand, within GFF1507 at 14.769 kb on - strand, within GFF1507 at 14.859 kb on + strand, within GFF1507 at 14.890 kb on - strand, within GFF1507 at 14.911 kb on + strand, within GFF1507 at 14.981 kb on - strand, within GFF1507 at 15.013 kb on + strand, within GFF1507 at 15.013 kb on + strand, within GFF1507 at 15.125 kb on - strand, within GFF1507 at 15.139 kb on - strand, within GFF1507 at 15.154 kb on + strand, within GFF1507 at 15.161 kb on - strand, within GFF1507 at 15.213 kb on + strand at 15.300 kb on - strand at 15.365 kb on - strand, within GFF1508 at 15.382 kb on + strand, within GFF1508 at 15.383 kb on - strand, within GFF1508 at 15.383 kb on - strand, within GFF1508 at 15.383 kb on - strand, within GFF1508 at 15.436 kb on + strand, within GFF1508 at 15.556 kb on + strand, within GFF1508 at 15.700 kb on + strand, within GFF1508 at 15.702 kb on + strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.902 kb on + strand, within GFF1508 at 15.903 kb on - strand, within GFF1508 at 16.004 kb on + strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.030 kb on - strand, within GFF1508 at 16.211 kb on - strand at 16.211 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 13,584 + GFF1505 0.85 +2.6 13,594 + GFF1505 0.86 +1.1 14,002 - GFF1506 0.32 -0.7 14,766 + GFF1507 0.34 -0.2 14,769 - GFF1507 0.34 -0.0 14,769 - GFF1507 0.34 -0.3 14,859 + GFF1507 0.47 -1.8 14,890 - GFF1507 0.51 -1.4 14,911 + GFF1507 0.54 +0.1 14,981 - GFF1507 0.64 +0.4 15,013 + GFF1507 0.68 -0.1 15,013 + GFF1507 0.68 -0.1 15,125 - GFF1507 0.84 -0.3 15,139 - GFF1507 0.86 +0.5 15,154 + GFF1507 0.88 +0.0 15,161 - GFF1507 0.89 +0.1 15,213 + +0.3 15,300 - -1.4 15,365 - GFF1508 0.15 -0.0 15,382 + GFF1508 0.16 -0.1 15,383 - GFF1508 0.16 -1.5 15,383 - GFF1508 0.16 -1.3 15,383 - GFF1508 0.16 -0.4 15,436 + GFF1508 0.22 +0.4 15,556 + GFF1508 0.34 +0.7 15,700 + GFF1508 0.48 +0.1 15,702 + GFF1508 0.48 -1.1 15,703 - GFF1508 0.48 +0.0 15,703 - GFF1508 0.48 +0.5 15,703 - GFF1508 0.48 +0.1 15,703 - GFF1508 0.48 -0.2 15,902 + GFF1508 0.68 -0.8 15,903 - GFF1508 0.68 -0.6 16,004 + GFF1508 0.78 -1.5 16,005 - GFF1508 0.78 -0.9 16,005 - GFF1508 0.78 +0.1 16,005 - GFF1508 0.78 -1.0 16,005 - GFF1508 0.78 +0.6 16,005 - GFF1508 0.78 -0.4 16,030 - GFF1508 0.81 +0.8 16,211 - -1.5 16,211 - -0.9
Or see this region's nucleotide sequence