Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1463 and GFF1464 are separated by 257 nucleotides GFF1464 and GFF1465 are separated by 151 nucleotides GFF1465 and GFF1466 are separated by 119 nucleotides
GFF1463 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 127,983 to 128,891
GFF1463
GFF1464 - PhnO protein, at 129,149 to 129,583
GFF1464
GFF1465 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 129,735 to 130,178
GFF1465
GFF1466 - Alkylphosphonate utilization operon protein PhnA, at 130,298 to 130,633
GFF1466
Position (kb) 129
130 Strain fitness (log2 ratio) -1
0
1 at 128.255 kb on + strand, within GFF1463 at 128.255 kb on + strand, within GFF1463 at 128.255 kb on + strand, within GFF1463 at 128.256 kb on - strand, within GFF1463 at 128.293 kb on + strand, within GFF1463 at 128.333 kb on + strand, within GFF1463 at 128.403 kb on + strand, within GFF1463 at 128.480 kb on + strand, within GFF1463 at 128.481 kb on - strand, within GFF1463 at 128.594 kb on + strand, within GFF1463 at 128.631 kb on + strand, within GFF1463 at 128.632 kb on - strand, within GFF1463 at 128.649 kb on + strand, within GFF1463 at 128.765 kb on + strand, within GFF1463 at 128.766 kb on - strand, within GFF1463 at 128.766 kb on - strand, within GFF1463 at 129.044 kb on + strand at 129.113 kb on + strand at 129.118 kb on + strand at 129.125 kb on + strand at 129.226 kb on + strand, within GFF1464 at 129.249 kb on - strand, within GFF1464 at 129.388 kb on + strand, within GFF1464 at 129.389 kb on - strand, within GFF1464 at 129.512 kb on + strand, within GFF1464 at 129.523 kb on + strand, within GFF1464 at 129.640 kb on + strand at 129.654 kb on - strand at 129.654 kb on - strand at 129.659 kb on - strand at 129.659 kb on - strand at 129.736 kb on + strand at 129.764 kb on + strand at 130.089 kb on - strand, within GFF1465 at 130.089 kb on - strand, within GFF1465 at 130.090 kb on + strand, within GFF1465 at 130.090 kb on + strand, within GFF1465 at 130.091 kb on - strand, within GFF1465 at 130.121 kb on - strand, within GFF1465 at 130.123 kb on + strand, within GFF1465 at 130.185 kb on + strand at 130.208 kb on + strand at 130.367 kb on - strand, within GFF1466 at 130.430 kb on - strand, within GFF1466
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 128,255 + GFF1463 0.30 +0.5 128,255 + GFF1463 0.30 -0.2 128,255 + GFF1463 0.30 -1.2 128,256 - GFF1463 0.30 -0.4 128,293 + GFF1463 0.34 -0.7 128,333 + GFF1463 0.39 -0.3 128,403 + GFF1463 0.46 -0.1 128,480 + GFF1463 0.55 +0.5 128,481 - GFF1463 0.55 -0.9 128,594 + GFF1463 0.67 +1.1 128,631 + GFF1463 0.71 +0.3 128,632 - GFF1463 0.71 -0.2 128,649 + GFF1463 0.73 +0.3 128,765 + GFF1463 0.86 +1.0 128,766 - GFF1463 0.86 -1.1 128,766 - GFF1463 0.86 +0.1 129,044 + +0.8 129,113 + -0.1 129,118 + +0.7 129,125 + -1.0 129,226 + GFF1464 0.18 -0.0 129,249 - GFF1464 0.23 +0.8 129,388 + GFF1464 0.55 +0.4 129,389 - GFF1464 0.55 +1.2 129,512 + GFF1464 0.83 +0.3 129,523 + GFF1464 0.86 +0.8 129,640 + +0.2 129,654 - +0.5 129,654 - +0.4 129,659 - -0.3 129,659 - +0.3 129,736 + -0.2 129,764 + +0.5 130,089 - GFF1465 0.80 +0.1 130,089 - GFF1465 0.80 -0.6 130,090 + GFF1465 0.80 +0.2 130,090 + GFF1465 0.80 +0.4 130,091 - GFF1465 0.80 -0.0 130,121 - GFF1465 0.87 +0.2 130,123 + GFF1465 0.87 -0.1 130,185 + -0.0 130,208 + -0.3 130,367 - GFF1466 0.21 -1.5 130,430 - GFF1466 0.39 -0.0
Or see this region's nucleotide sequence