Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1426 and GFF1427 are separated by 0 nucleotides GFF1427 and GFF1428 overlap by 14 nucleotides GFF1428 and GFF1429 are separated by 143 nucleotides GFF1429 and GFF1430 are separated by 247 nucleotides GFF1430 and GFF1431 are separated by 209 nucleotides
GFF1426 - Protein yjbR, at 72,075 to 72,431
GFF1426
GFF1427 - Putative inner membrane protein, at 72,432 to 72,770
GFF1427
GFF1428 - Putative outer membrane lipoprotein, at 72,757 to 73,200
GFF1428
GFF1429 - Excinuclease ABC subunit A, at 73,344 to 76,169
GFF1429
GFF1430 - Single-stranded DNA-binding protein, at 76,417 to 76,947
GFF1430
GFF1431 - FIG01048353: hypothetical protein, at 77,157 to 77,312
GFF1431
Position (kb) 73
74
75
76
77 Strain fitness (log2 ratio) -2
-1
0
1 at 72.373 kb on - strand, within GFF1426 at 72.433 kb on + strand at 72.485 kb on + strand, within GFF1427 at 72.522 kb on - strand, within GFF1427 at 72.532 kb on - strand, within GFF1427 at 72.532 kb on - strand, within GFF1427 at 72.566 kb on + strand, within GFF1427 at 72.674 kb on + strand, within GFF1427 at 72.674 kb on + strand, within GFF1427 at 72.674 kb on + strand, within GFF1427 at 72.675 kb on - strand, within GFF1427 at 72.676 kb on + strand, within GFF1427 at 72.676 kb on + strand, within GFF1427 at 72.745 kb on + strand at 72.750 kb on + strand at 72.750 kb on + strand at 72.751 kb on - strand at 72.758 kb on + strand at 72.820 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.875 kb on + strand, within GFF1428 at 72.876 kb on - strand, within GFF1428 at 72.876 kb on - strand, within GFF1428 at 72.879 kb on + strand, within GFF1428 at 72.923 kb on + strand, within GFF1428 at 73.100 kb on - strand, within GFF1428 at 73.105 kb on + strand, within GFF1428 at 73.105 kb on + strand, within GFF1428 at 73.105 kb on + strand, within GFF1428 at 73.106 kb on - strand, within GFF1428 at 73.361 kb on + strand at 73.609 kb on + strand at 73.609 kb on + strand at 73.711 kb on + strand, within GFF1429 at 73.711 kb on + strand, within GFF1429 at 73.712 kb on + strand, within GFF1429 at 73.712 kb on - strand, within GFF1429 at 73.723 kb on - strand, within GFF1429 at 73.746 kb on - strand, within GFF1429 at 73.746 kb on - strand, within GFF1429 at 73.831 kb on + strand, within GFF1429 at 73.836 kb on - strand, within GFF1429 at 73.859 kb on - strand, within GFF1429 at 73.892 kb on - strand, within GFF1429 at 73.892 kb on - strand, within GFF1429 at 73.923 kb on - strand, within GFF1429 at 74.144 kb on + strand, within GFF1429 at 74.265 kb on - strand, within GFF1429 at 74.285 kb on + strand, within GFF1429 at 74.429 kb on + strand, within GFF1429 at 74.552 kb on + strand, within GFF1429 at 74.641 kb on + strand, within GFF1429 at 74.642 kb on - strand, within GFF1429 at 74.687 kb on + strand, within GFF1429 at 74.688 kb on - strand, within GFF1429 at 74.880 kb on - strand, within GFF1429 at 74.894 kb on + strand, within GFF1429 at 74.907 kb on + strand, within GFF1429 at 74.908 kb on - strand, within GFF1429 at 74.964 kb on - strand, within GFF1429 at 75.030 kb on - strand, within GFF1429 at 75.033 kb on - strand, within GFF1429 at 75.092 kb on - strand, within GFF1429 at 75.138 kb on - strand, within GFF1429 at 75.138 kb on - strand, within GFF1429 at 75.175 kb on - strand, within GFF1429 at 75.186 kb on - strand, within GFF1429 at 75.186 kb on - strand, within GFF1429 at 75.308 kb on - strand, within GFF1429 at 75.576 kb on - strand, within GFF1429 at 75.704 kb on + strand, within GFF1429 at 75.705 kb on - strand, within GFF1429 at 75.850 kb on + strand, within GFF1429 at 75.869 kb on + strand, within GFF1429 at 75.870 kb on - strand, within GFF1429 at 75.944 kb on + strand at 76.133 kb on + strand at 76.236 kb on + strand at 76.303 kb on + strand at 76.346 kb on + strand at 77.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 72,373 - GFF1426 0.83 -0.6 72,433 + +0.5 72,485 + GFF1427 0.16 +0.6 72,522 - GFF1427 0.27 -0.1 72,532 - GFF1427 0.29 -0.3 72,532 - GFF1427 0.29 -0.3 72,566 + GFF1427 0.40 -2.4 72,674 + GFF1427 0.71 +0.1 72,674 + GFF1427 0.71 -0.2 72,674 + GFF1427 0.71 +0.3 72,675 - GFF1427 0.72 +0.1 72,676 + GFF1427 0.72 -0.1 72,676 + GFF1427 0.72 -0.3 72,745 + +0.4 72,750 + +0.4 72,750 + +0.1 72,751 - +0.4 72,758 + +0.2 72,820 + GFF1428 0.14 +0.4 72,875 + GFF1428 0.27 +0.6 72,875 + GFF1428 0.27 +1.0 72,875 + GFF1428 0.27 +0.1 72,875 + GFF1428 0.27 +0.4 72,876 - GFF1428 0.27 -0.4 72,876 - GFF1428 0.27 -0.1 72,879 + GFF1428 0.27 +0.7 72,923 + GFF1428 0.37 -1.0 73,100 - GFF1428 0.77 -1.6 73,105 + GFF1428 0.78 +1.4 73,105 + GFF1428 0.78 +0.5 73,105 + GFF1428 0.78 +0.1 73,106 - GFF1428 0.79 +1.3 73,361 + -0.1 73,609 + +0.1 73,609 + +0.9 73,711 + GFF1429 0.13 -0.6 73,711 + GFF1429 0.13 -1.8 73,712 + GFF1429 0.13 -0.4 73,712 - GFF1429 0.13 +0.5 73,723 - GFF1429 0.13 +0.8 73,746 - GFF1429 0.14 -0.7 73,746 - GFF1429 0.14 -0.3 73,831 + GFF1429 0.17 -1.5 73,836 - GFF1429 0.17 +0.1 73,859 - GFF1429 0.18 -0.1 73,892 - GFF1429 0.19 -1.1 73,892 - GFF1429 0.19 -0.1 73,923 - GFF1429 0.20 -1.8 74,144 + GFF1429 0.28 -0.3 74,265 - GFF1429 0.33 -0.1 74,285 + GFF1429 0.33 +0.2 74,429 + GFF1429 0.38 -2.4 74,552 + GFF1429 0.43 +0.7 74,641 + GFF1429 0.46 -0.1 74,642 - GFF1429 0.46 -1.1 74,687 + GFF1429 0.48 +0.4 74,688 - GFF1429 0.48 -2.1 74,880 - GFF1429 0.54 +0.2 74,894 + GFF1429 0.55 -1.2 74,907 + GFF1429 0.55 -1.1 74,908 - GFF1429 0.55 +0.2 74,964 - GFF1429 0.57 -0.6 75,030 - GFF1429 0.60 -0.1 75,033 - GFF1429 0.60 +0.7 75,092 - GFF1429 0.62 -0.5 75,138 - GFF1429 0.63 -0.2 75,138 - GFF1429 0.63 +0.4 75,175 - GFF1429 0.65 -0.2 75,186 - GFF1429 0.65 -0.0 75,186 - GFF1429 0.65 -1.3 75,308 - GFF1429 0.69 +0.1 75,576 - GFF1429 0.79 +0.3 75,704 + GFF1429 0.84 +0.6 75,705 - GFF1429 0.84 -1.3 75,850 + GFF1429 0.89 -0.1 75,869 + GFF1429 0.89 -1.1 75,870 - GFF1429 0.89 +0.4 75,944 + -0.1 76,133 + -1.4 76,236 + +0.2 76,303 + +0.2 76,346 + -0.1 77,042 - +0.3
Or see this region's nucleotide sequence