Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1417 and GFF1418 are separated by 87 nucleotides GFF1418 and GFF1419 are separated by 167 nucleotides GFF1419 and GFF1420 are separated by 174 nucleotides
GFF1417 - FIG01200701: possible membrane protein, at 62,693 to 64,003
GFF1417
GFF1418 - tRNA dihydrouridine synthase A (EC 1.-.-.-), at 64,091 to 65,089
GFF1418
GFF1419 - Phage shock protein G, at 65,257 to 65,499
GFF1419
GFF1420 - Quinone oxidoreductase (EC 1.6.5.5), at 65,674 to 66,657
GFF1420
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.186 kb on - strand, within GFF1417 at 63.357 kb on + strand, within GFF1417 at 63.358 kb on - strand, within GFF1417 at 63.455 kb on - strand, within GFF1417 at 63.458 kb on + strand, within GFF1417 at 63.500 kb on - strand, within GFF1417 at 63.629 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.646 kb on + strand, within GFF1417 at 63.646 kb on + strand, within GFF1417 at 63.677 kb on - strand, within GFF1417 at 63.677 kb on - strand, within GFF1417 at 63.677 kb on - strand, within GFF1417 at 63.681 kb on - strand, within GFF1417 at 63.775 kb on + strand, within GFF1417 at 63.797 kb on + strand, within GFF1417 at 63.797 kb on + strand, within GFF1417 at 63.940 kb on - strand at 63.989 kb on - strand at 63.989 kb on - strand at 63.989 kb on - strand at 64.049 kb on - strand at 64.127 kb on + strand at 64.147 kb on + strand at 64.253 kb on - strand, within GFF1418 at 64.274 kb on - strand, within GFF1418 at 64.365 kb on - strand, within GFF1418 at 64.506 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.542 kb on - strand, within GFF1418 at 64.542 kb on - strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.899 kb on - strand, within GFF1418 at 64.899 kb on - strand, within GFF1418 at 64.907 kb on - strand, within GFF1418 at 64.934 kb on + strand, within GFF1418 at 65.050 kb on - strand at 65.156 kb on - strand at 65.268 kb on - strand at 65.297 kb on + strand, within GFF1419 at 65.345 kb on + strand, within GFF1419 at 65.503 kb on - strand at 65.549 kb on - strand at 65.672 kb on + strand at 65.673 kb on - strand at 65.753 kb on + strand at 65.848 kb on - strand, within GFF1420 at 65.850 kb on + strand, within GFF1420 at 65.850 kb on + strand, within GFF1420 at 65.851 kb on - strand, within GFF1420 at 65.852 kb on + strand, within GFF1420 at 65.853 kb on - strand, within GFF1420 at 66.018 kb on - strand, within GFF1420 at 66.088 kb on - strand, within GFF1420
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 63,186 - GFF1417 0.38 +0.6 63,357 + GFF1417 0.51 -0.0 63,358 - GFF1417 0.51 -0.2 63,455 - GFF1417 0.58 +0.3 63,458 + GFF1417 0.58 +0.6 63,500 - GFF1417 0.62 -0.1 63,629 - GFF1417 0.71 -0.2 63,633 - GFF1417 0.72 -0.0 63,633 - GFF1417 0.72 +0.5 63,633 - GFF1417 0.72 +0.2 63,633 - GFF1417 0.72 +0.2 63,646 + GFF1417 0.73 -0.1 63,646 + GFF1417 0.73 +0.2 63,677 - GFF1417 0.75 -0.1 63,677 - GFF1417 0.75 -0.1 63,677 - GFF1417 0.75 +0.2 63,681 - GFF1417 0.75 +0.1 63,775 + GFF1417 0.83 +0.3 63,797 + GFF1417 0.84 -2.4 63,797 + GFF1417 0.84 -0.3 63,940 - +0.2 63,989 - +1.1 63,989 - -0.0 63,989 - +0.6 64,049 - -0.0 64,127 + -0.8 64,147 + -0.0 64,253 - GFF1418 0.16 -1.1 64,274 - GFF1418 0.18 +0.3 64,365 - GFF1418 0.27 -1.4 64,506 + GFF1418 0.42 +0.6 64,541 + GFF1418 0.45 +0.3 64,541 + GFF1418 0.45 +1.0 64,541 + GFF1418 0.45 +0.5 64,541 + GFF1418 0.45 +0.3 64,542 - GFF1418 0.45 +0.5 64,542 - GFF1418 0.45 -0.0 64,898 + GFF1418 0.81 -0.0 64,898 + GFF1418 0.81 +0.8 64,898 + GFF1418 0.81 +0.5 64,899 - GFF1418 0.81 +0.9 64,899 - GFF1418 0.81 +0.6 64,907 - GFF1418 0.82 -0.9 64,934 + GFF1418 0.84 +0.1 65,050 - +0.1 65,156 - +0.2 65,268 - -1.1 65,297 + GFF1419 0.16 +0.6 65,345 + GFF1419 0.36 +0.6 65,503 - -0.4 65,549 - +0.3 65,672 + +0.1 65,673 - -0.1 65,753 + +0.0 65,848 - GFF1420 0.18 +0.2 65,850 + GFF1420 0.18 +0.5 65,850 + GFF1420 0.18 -0.1 65,851 - GFF1420 0.18 +0.2 65,852 + GFF1420 0.18 +1.2 65,853 - GFF1420 0.18 +0.0 66,018 - GFF1420 0.35 +1.9 66,088 - GFF1420 0.42 +0.2
Or see this region's nucleotide sequence