Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF128

Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF127 and GFF128 are separated by 91 nucleotidesGFF128 and GFF129 overlap by 4 nucleotidesGFF129 and GFF130 are separated by 87 nucleotides GFF127 - Alpha-L-arabinofuranosidase II precursor (EC 3.2.1.55), at 117,280 to 118,230 GFF127 GFF128 - AmpE protein, at 118,322 to 119,176 GFF128 GFF129 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD, at 119,173 to 119,736 GFF129 GFF130 - Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19), at 119,824 to 120,717 GFF130 Position (kb) 118 119 120Strain fitness (log2 ratio) -2 -1 0 1at 117.328 kb on - strandat 117.333 kb on - strandat 117.333 kb on - strandat 117.349 kb on + strandat 117.353 kb on - strandat 117.357 kb on + strandat 117.451 kb on - strand, within GFF127at 117.526 kb on + strand, within GFF127at 117.559 kb on - strand, within GFF127at 117.897 kb on + strand, within GFF127at 117.904 kb on + strand, within GFF127at 117.959 kb on + strand, within GFF127at 117.993 kb on + strand, within GFF127at 117.994 kb on - strand, within GFF127at 118.062 kb on + strand, within GFF127at 118.228 kb on + strandat 118.328 kb on + strandat 118.328 kb on + strandat 118.339 kb on - strandat 118.418 kb on + strand, within GFF128at 118.479 kb on + strand, within GFF128at 118.479 kb on + strand, within GFF128at 118.489 kb on + strand, within GFF128at 118.490 kb on - strand, within GFF128at 118.490 kb on - strand, within GFF128at 118.554 kb on + strand, within GFF128at 118.554 kb on + strand, within GFF128at 118.560 kb on - strand, within GFF128at 118.808 kb on - strand, within GFF128at 118.808 kb on - strand, within GFF128at 118.905 kb on + strand, within GFF128at 118.906 kb on - strand, within GFF128at 118.911 kb on - strand, within GFF128at 118.934 kb on - strand, within GFF128at 118.983 kb on - strand, within GFF128at 118.994 kb on + strand, within GFF128at 119.084 kb on + strand, within GFF128at 119.156 kb on + strandat 119.164 kb on + strandat 119.307 kb on - strand, within GFF129at 119.349 kb on - strand, within GFF129at 119.477 kb on + strand, within GFF129at 119.477 kb on + strand, within GFF129at 119.477 kb on + strand, within GFF129at 119.478 kb on - strand, within GFF129at 119.632 kb on - strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.634 kb on + strand, within GFF129at 119.635 kb on - strand, within GFF129at 119.764 kb on + strandat 119.765 kb on - strandat 119.765 kb on - strandat 119.778 kb on + strandat 119.794 kb on + strandat 119.795 kb on - strandat 119.839 kb on + strandat 119.839 kb on + strandat 119.982 kb on + strand, within GFF130

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
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117,328 - -0.5
117,333 - +0.4
117,333 - -1.4
117,349 + -0.6
117,353 - +0.0
117,357 + +0.1
117,451 - GFF127 0.18 +0.1
117,526 + GFF127 0.26 -0.1
117,559 - GFF127 0.29 +0.4
117,897 + GFF127 0.65 +0.3
117,904 + GFF127 0.66 +0.1
117,959 + GFF127 0.71 -0.3
117,993 + GFF127 0.75 -0.7
117,994 - GFF127 0.75 +0.2
118,062 + GFF127 0.82 -0.3
118,228 + +0.5
118,328 + -2.3
118,328 + +0.2
118,339 - -0.0
118,418 + GFF128 0.11 +0.4
118,479 + GFF128 0.18 -0.1
118,479 + GFF128 0.18 +0.5
118,489 + GFF128 0.20 -0.1
118,490 - GFF128 0.20 -0.4
118,490 - GFF128 0.20 +1.0
118,554 + GFF128 0.27 +0.6
118,554 + GFF128 0.27 +0.2
118,560 - GFF128 0.28 +0.7
118,808 - GFF128 0.57 -0.0
118,808 - GFF128 0.57 -0.4
118,905 + GFF128 0.68 +0.0
118,906 - GFF128 0.68 +0.4
118,911 - GFF128 0.69 -0.3
118,934 - GFF128 0.72 +0.8
118,983 - GFF128 0.77 +0.0
118,994 + GFF128 0.79 +0.3
119,084 + GFF128 0.89 +0.5
119,156 + +0.2
119,164 + +0.1
119,307 - GFF129 0.24 +0.2
119,349 - GFF129 0.31 +0.7
119,477 + GFF129 0.54 +0.5
119,477 + GFF129 0.54 +0.7
119,477 + GFF129 0.54 +1.3
119,478 - GFF129 0.54 +0.3
119,632 - GFF129 0.81 +0.4
119,634 + GFF129 0.82 -0.8
119,634 + GFF129 0.82 +0.7
119,634 + GFF129 0.82 +1.0
119,634 + GFF129 0.82 +0.3
119,635 - GFF129 0.82 +1.0
119,764 + +0.7
119,765 - -0.4
119,765 - -0.2
119,778 + -0.9
119,794 + +0.0
119,795 - +0.9
119,839 + -0.1
119,839 + -0.7
119,982 + GFF130 0.18 -0.7

Or see this region's nucleotide sequence