Experiment: LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1184 and GFF1185 are separated by 376 nucleotides GFF1185 and GFF1186 are separated by 8 nucleotides GFF1186 and GFF1187 overlap by 1 nucleotides GFF1187 and GFF1188 are separated by 78 nucleotides
GFF1184 - IncF plasmid conjugative transfer surface exclusion protein TraT, at 29,499 to 30,230
GFF1184
GFF1185 - IncF plasmid conjugative transfer protein TraD, at 30,607 to 32,850
GFF1185
GFF1186 - VapC toxin protein, at 32,859 to 33,257
GFF1186
GFF1187 - VapB protein (antitoxin to VapC), at 33,257 to 33,487
GFF1187
GFF1188 - IncF plasmid conjugative transfer DNA-nicking and unwinding protein TraI, at 33,566 to 38,824
GFF1188
Position (kb)
30
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 29.658 kb on + strand, within GFF1184 at 29.658 kb on + strand, within GFF1184 at 29.676 kb on - strand, within GFF1184 at 29.739 kb on + strand, within GFF1184 at 29.742 kb on - strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.769 kb on + strand, within GFF1184 at 29.773 kb on - strand, within GFF1184 at 29.895 kb on + strand, within GFF1184 at 29.895 kb on + strand, within GFF1184 at 29.896 kb on - strand, within GFF1184 at 29.982 kb on - strand, within GFF1184 at 29.985 kb on - strand, within GFF1184 at 30.119 kb on + strand, within GFF1184 at 30.120 kb on - strand, within GFF1184 at 30.120 kb on - strand, within GFF1184 at 30.123 kb on + strand, within GFF1184 at 30.547 kb on - strand at 30.660 kb on + strand at 30.785 kb on - strand at 30.825 kb on + strand at 30.825 kb on + strand at 31.045 kb on - strand, within GFF1185 at 31.146 kb on + strand, within GFF1185 at 31.197 kb on + strand, within GFF1185 at 31.198 kb on - strand, within GFF1185 at 31.228 kb on + strand, within GFF1185 at 31.228 kb on + strand, within GFF1185 at 31.228 kb on + strand, within GFF1185 at 31.228 kb on + strand, within GFF1185 at 31.228 kb on + strand, within GFF1185 at 31.228 kb on + strand, within GFF1185 at 31.229 kb on - strand, within GFF1185 at 31.229 kb on - strand, within GFF1185 at 31.261 kb on + strand, within GFF1185 at 31.261 kb on + strand, within GFF1185 at 31.262 kb on - strand, within GFF1185 at 31.262 kb on - strand, within GFF1185 at 31.356 kb on - strand, within GFF1185 at 31.477 kb on + strand, within GFF1185 at 31.489 kb on - strand, within GFF1185 at 31.508 kb on - strand, within GFF1185 at 31.512 kb on + strand, within GFF1185 at 31.512 kb on + strand, within GFF1185 at 31.561 kb on + strand, within GFF1185 at 31.561 kb on + strand, within GFF1185 at 31.562 kb on - strand, within GFF1185 at 31.562 kb on - strand, within GFF1185 at 31.645 kb on + strand, within GFF1185 at 31.645 kb on + strand, within GFF1185 at 31.649 kb on + strand, within GFF1185 at 31.955 kb on - strand, within GFF1185 at 31.993 kb on - strand, within GFF1185 at 31.993 kb on - strand, within GFF1185 at 31.996 kb on + strand, within GFF1185 at 31.996 kb on + strand, within GFF1185 at 31.996 kb on + strand, within GFF1185 at 31.997 kb on - strand, within GFF1185 at 31.997 kb on - strand, within GFF1185 at 32.066 kb on - strand, within GFF1185 at 32.067 kb on + strand, within GFF1185 at 32.067 kb on + strand, within GFF1185 at 32.067 kb on + strand, within GFF1185 at 32.067 kb on + strand, within GFF1185 at 32.081 kb on - strand, within GFF1185 at 32.081 kb on - strand, within GFF1185 at 32.137 kb on + strand, within GFF1185 at 32.207 kb on - strand, within GFF1185 at 32.243 kb on - strand, within GFF1185 at 32.290 kb on + strand, within GFF1185 at 32.617 kb on - strand, within GFF1185 at 32.691 kb on + strand at 32.725 kb on + strand at 32.793 kb on + strand at 33.044 kb on - strand, within GFF1186 at 33.124 kb on + strand, within GFF1186 at 33.156 kb on - strand, within GFF1186 at 33.233 kb on + strand at 33.234 kb on - strand at 33.234 kb on - strand at 33.236 kb on + strand at 33.246 kb on - strand at 33.304 kb on + strand, within GFF1187 at 33.368 kb on - strand, within GFF1187 at 33.470 kb on + strand at 33.471 kb on - strand at 33.505 kb on + strand at 33.506 kb on - strand at 33.512 kb on + strand at 33.513 kb on - strand at 33.517 kb on - strand at 33.574 kb on + strand at 33.575 kb on - strand at 33.614 kb on + strand at 33.615 kb on - strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.628 kb on + strand at 33.629 kb on - strand at 33.632 kb on + strand at 33.633 kb on - strand at 33.635 kb on + strand at 33.636 kb on - strand at 33.636 kb on - strand at 33.636 kb on - strand at 33.636 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (wt new) 0.75 MOI remove 29,658 + GFF1184 0.22 +0.8 29,658 + GFF1184 0.22 +0.2 29,676 - GFF1184 0.24 -0.4 29,739 + GFF1184 0.33 -1.5 29,742 - GFF1184 0.33 -0.1 29,769 + GFF1184 0.37 +0.2 29,769 + GFF1184 0.37 +1.5 29,769 + GFF1184 0.37 -0.2 29,773 - GFF1184 0.37 +0.4 29,895 + GFF1184 0.54 +0.0 29,895 + GFF1184 0.54 -0.0 29,896 - GFF1184 0.54 -0.5 29,982 - GFF1184 0.66 -0.3 29,985 - GFF1184 0.66 -0.4 30,119 + GFF1184 0.85 -0.1 30,120 - GFF1184 0.85 -0.1 30,120 - GFF1184 0.85 -0.7 30,123 + GFF1184 0.85 +0.2 30,547 - +0.1 30,660 + +0.6 30,785 - +0.1 30,825 + +0.3 30,825 + +0.1 31,045 - GFF1185 0.20 +1.0 31,146 + GFF1185 0.24 +0.3 31,197 + GFF1185 0.26 +0.1 31,198 - GFF1185 0.26 -0.4 31,228 + GFF1185 0.28 +1.9 31,228 + GFF1185 0.28 +0.0 31,228 + GFF1185 0.28 -0.4 31,228 + GFF1185 0.28 -0.3 31,228 + GFF1185 0.28 +0.5 31,228 + GFF1185 0.28 +0.2 31,229 - GFF1185 0.28 -0.0 31,229 - GFF1185 0.28 -1.3 31,261 + GFF1185 0.29 +0.1 31,261 + GFF1185 0.29 -0.0 31,262 - GFF1185 0.29 -1.0 31,262 - GFF1185 0.29 +0.7 31,356 - GFF1185 0.33 +0.5 31,477 + GFF1185 0.39 -2.0 31,489 - GFF1185 0.39 -0.4 31,508 - GFF1185 0.40 -0.0 31,512 + GFF1185 0.40 -0.3 31,512 + GFF1185 0.40 +1.1 31,561 + GFF1185 0.43 +0.4 31,561 + GFF1185 0.43 +0.2 31,562 - GFF1185 0.43 -0.1 31,562 - GFF1185 0.43 +0.5 31,645 + GFF1185 0.46 +0.3 31,645 + GFF1185 0.46 +0.0 31,649 + GFF1185 0.46 -0.9 31,955 - GFF1185 0.60 -0.5 31,993 - GFF1185 0.62 +0.7 31,993 - GFF1185 0.62 +1.2 31,996 + GFF1185 0.62 +0.1 31,996 + GFF1185 0.62 +0.3 31,996 + GFF1185 0.62 +0.6 31,997 - GFF1185 0.62 -0.2 31,997 - GFF1185 0.62 +0.0 32,066 - GFF1185 0.65 +0.1 32,067 + GFF1185 0.65 +0.1 32,067 + GFF1185 0.65 +0.9 32,067 + GFF1185 0.65 +0.1 32,067 + GFF1185 0.65 +1.1 32,081 - GFF1185 0.66 +0.2 32,081 - GFF1185 0.66 -0.4 32,137 + GFF1185 0.68 +0.4 32,207 - GFF1185 0.71 -0.7 32,243 - GFF1185 0.73 +1.1 32,290 + GFF1185 0.75 +0.3 32,617 - GFF1185 0.90 -0.0 32,691 + -0.4 32,725 + +0.7 32,793 + -0.2 33,044 - GFF1186 0.46 -0.1 33,124 + GFF1186 0.66 -0.4 33,156 - GFF1186 0.74 -0.4 33,233 + -0.4 33,234 - +0.0 33,234 - +1.0 33,236 + -0.4 33,246 - +0.1 33,304 + GFF1187 0.20 +0.3 33,368 - GFF1187 0.48 -0.3 33,470 + -0.1 33,471 - -0.3 33,505 + +0.6 33,506 - +0.3 33,512 + +0.6 33,513 - -1.1 33,517 - +0.6 33,574 + -0.0 33,575 - -0.5 33,614 + +1.9 33,615 - -0.0 33,628 + -0.3 33,628 + -0.8 33,628 + -0.6 33,628 + -0.6 33,628 + -0.4 33,629 - -0.9 33,632 + -0.2 33,633 - +1.1 33,635 + -0.3 33,636 - +0.0 33,636 - -0.7 33,636 - +0.0 33,636 - -0.8
Or see this region's nucleotide sequence