Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF995 and GFF996 are separated by 204 nucleotides GFF996 and GFF997 are separated by 74 nucleotides GFF997 and GFF998 are separated by 73 nucleotides GFF998 and GFF999 are separated by 68 nucleotides GFF999 and GFF1000 are separated by 26 nucleotides
GFF995 - Succinate-semialdehyde dehydrogenase [NAD] (EC 1.2.1.24); Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 177,810 to 179,180
GFF995
GFF996 - FIG074102: hypothetical protein, at 179,385 to 179,549
GFF996
GFF997 - Putative inner membrane protein, at 179,624 to 180,325
GFF997
GFF998 - FIG00638399: hypothetical protein, at 180,399 to 180,731
GFF998
GFF999 - hypothetical protein, at 180,800 to 180,931
GFF999
GFF1000 - Type I restriction-modification system, specificity subunit S (EC 3.1.21.3), at 180,958 to 182,316
GFF1000
Position (kb) 179
180
181 Strain fitness (log2 ratio) -1
0
1 at 178.856 kb on + strand, within GFF995 at 178.859 kb on + strand, within GFF995 at 178.914 kb on + strand, within GFF995 at 178.914 kb on + strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 178.915 kb on - strand, within GFF995 at 179.165 kb on - strand at 179.179 kb on - strand at 179.289 kb on + strand at 179.370 kb on + strand at 180.018 kb on - strand, within GFF997 at 180.299 kb on - strand at 180.359 kb on + strand at 180.534 kb on - strand, within GFF998 at 180.631 kb on + strand, within GFF998 at 180.653 kb on - strand, within GFF998 at 180.714 kb on - strand at 180.726 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 178,856 + GFF995 0.76 +0.4 178,859 + GFF995 0.77 +0.0 178,914 + GFF995 0.81 -0.2 178,914 + GFF995 0.81 +0.4 178,915 - GFF995 0.81 +0.1 178,915 - GFF995 0.81 +0.9 178,915 - GFF995 0.81 +0.3 178,915 - GFF995 0.81 +0.6 179,165 - -0.9 179,179 - -1.0 179,289 + -0.1 179,370 + -0.1 180,018 - GFF997 0.56 +0.3 180,299 - -0.2 180,359 + -0.2 180,534 - GFF998 0.41 -0.1 180,631 + GFF998 0.70 +1.3 180,653 - GFF998 0.76 +0.0 180,714 - +0.3 180,726 - +0.1
Or see this region's nucleotide sequence