Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF979 and GFF980 are separated by 0 nucleotides GFF980 and GFF981 are separated by 296 nucleotides GFF981 and GFF982 are separated by 352 nucleotides
GFF979 - Uxu operon transcriptional regulator, at 164,452 to 165,177
GFF979
GFF980 - Tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 165,178 to 166,191
GFF980
GFF981 - FIG01045355: hypothetical protein, at 166,488 to 166,640
GFF981
GFF982 - Putative cytoplasmic protein USSDB7A, at 166,993 to 167,439
GFF982
Position (kb) 165
166
167 Strain fitness (log2 ratio) -1
0
1 at 164.351 kb on + strand at 164.361 kb on - strand at 164.361 kb on - strand at 164.361 kb on - strand at 164.545 kb on - strand, within GFF979 at 164.618 kb on - strand, within GFF979 at 164.674 kb on - strand, within GFF979 at 164.773 kb on - strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.889 kb on + strand, within GFF979 at 164.890 kb on - strand, within GFF979 at 164.890 kb on - strand, within GFF979 at 164.958 kb on + strand, within GFF979 at 165.125 kb on - strand at 165.137 kb on - strand at 165.182 kb on - strand at 165.190 kb on + strand at 165.218 kb on - strand at 165.227 kb on - strand at 165.460 kb on - strand, within GFF980 at 165.521 kb on + strand, within GFF980 at 165.577 kb on - strand, within GFF980 at 165.774 kb on + strand, within GFF980 at 165.774 kb on + strand, within GFF980 at 165.798 kb on - strand, within GFF980 at 165.798 kb on - strand, within GFF980 at 165.857 kb on - strand, within GFF980 at 165.862 kb on - strand, within GFF980 at 165.894 kb on + strand, within GFF980 at 165.903 kb on - strand, within GFF980 at 166.032 kb on + strand, within GFF980 at 166.076 kb on + strand, within GFF980 at 166.077 kb on - strand, within GFF980 at 166.077 kb on - strand, within GFF980 at 166.205 kb on - strand at 166.205 kb on - strand at 166.212 kb on - strand at 166.359 kb on + strand at 166.435 kb on + strand at 166.787 kb on + strand at 166.843 kb on - strand at 167.125 kb on + strand, within GFF982 at 167.125 kb on + strand, within GFF982 at 167.125 kb on + strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.127 kb on + strand, within GFF982 at 167.128 kb on - strand, within GFF982 at 167.166 kb on + strand, within GFF982 at 167.179 kb on + strand, within GFF982
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 164,351 + +1.0 164,361 - -0.3 164,361 - -1.6 164,361 - -1.5 164,545 - GFF979 0.13 +0.5 164,618 - GFF979 0.23 -0.7 164,674 - GFF979 0.31 +0.1 164,773 - GFF979 0.44 -0.4 164,889 + GFF979 0.60 -0.4 164,889 + GFF979 0.60 +0.1 164,889 + GFF979 0.60 -0.1 164,890 - GFF979 0.60 -0.2 164,890 - GFF979 0.60 -0.3 164,958 + GFF979 0.70 -0.2 165,125 - -0.2 165,137 - -0.3 165,182 - -0.3 165,190 + -0.2 165,218 - -0.3 165,227 - -0.4 165,460 - GFF980 0.28 +0.7 165,521 + GFF980 0.34 -0.2 165,577 - GFF980 0.39 -0.6 165,774 + GFF980 0.59 +0.1 165,774 + GFF980 0.59 +0.1 165,798 - GFF980 0.61 +0.2 165,798 - GFF980 0.61 -0.0 165,857 - GFF980 0.67 +0.4 165,862 - GFF980 0.67 +1.3 165,894 + GFF980 0.71 +0.4 165,903 - GFF980 0.71 -0.3 166,032 + GFF980 0.84 -0.0 166,076 + GFF980 0.89 +0.2 166,077 - GFF980 0.89 +0.4 166,077 - GFF980 0.89 +1.0 166,205 - +0.5 166,205 - -0.2 166,212 - -0.1 166,359 + -0.5 166,435 + +0.1 166,787 + -0.1 166,843 - -0.2 167,125 + GFF982 0.30 -0.5 167,125 + GFF982 0.30 -1.7 167,125 + GFF982 0.30 -0.5 167,126 - GFF982 0.30 -0.7 167,126 - GFF982 0.30 -0.3 167,126 - GFF982 0.30 -1.4 167,127 + GFF982 0.30 -0.8 167,128 - GFF982 0.30 -0.5 167,166 + GFF982 0.39 -0.0 167,179 + GFF982 0.42 -0.8
Or see this region's nucleotide sequence