Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF891 and GFF892 are separated by 96 nucleotides GFF892 and GFF893 are separated by 0 nucleotides GFF893 and GFF894 overlap by 4 nucleotides
GFF891 - Myo-inositol 2-dehydrogenase 1 (EC 1.1.1.18), at 71,258 to 72,268
GFF891
GFF892 - lysosomal glucosyl ceramidase-like protein, at 72,365 to 73,708
GFF892
GFF893 - Inosose isomerase (EC 5.3.99.-), at 73,709 to 74,542
GFF893
GFF894 - FIG01046005: hypothetical protein, at 74,539 to 75,705
GFF894
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2 at 71.396 kb on - strand, within GFF891 at 71.400 kb on - strand, within GFF891 at 71.450 kb on - strand, within GFF891 at 71.525 kb on - strand, within GFF891 at 71.765 kb on - strand, within GFF891 at 71.765 kb on - strand, within GFF891 at 71.768 kb on + strand, within GFF891 at 71.769 kb on - strand, within GFF891 at 72.047 kb on - strand, within GFF891 at 72.082 kb on + strand, within GFF891 at 72.083 kb on - strand, within GFF891 at 72.170 kb on + strand at 72.462 kb on + strand at 72.463 kb on - strand at 72.663 kb on - strand, within GFF892 at 72.663 kb on - strand, within GFF892 at 72.811 kb on + strand, within GFF892 at 72.820 kb on + strand, within GFF892 at 72.893 kb on + strand, within GFF892 at 72.894 kb on - strand, within GFF892 at 72.986 kb on + strand, within GFF892 at 72.986 kb on + strand, within GFF892 at 72.987 kb on - strand, within GFF892 at 72.988 kb on + strand, within GFF892 at 72.989 kb on - strand, within GFF892 at 73.070 kb on + strand, within GFF892 at 73.172 kb on + strand, within GFF892 at 73.197 kb on + strand, within GFF892 at 73.297 kb on + strand, within GFF892 at 73.466 kb on + strand, within GFF892 at 73.467 kb on - strand, within GFF892 at 73.467 kb on - strand, within GFF892 at 73.491 kb on - strand, within GFF892 at 73.592 kb on + strand at 73.592 kb on + strand at 73.592 kb on + strand at 73.592 kb on + strand at 73.640 kb on - strand at 73.678 kb on + strand at 73.679 kb on - strand at 73.687 kb on - strand at 73.799 kb on + strand, within GFF893 at 73.819 kb on + strand, within GFF893 at 73.820 kb on - strand, within GFF893 at 73.820 kb on - strand, within GFF893 at 73.846 kb on + strand, within GFF893 at 73.852 kb on - strand, within GFF893 at 73.888 kb on + strand, within GFF893 at 73.889 kb on - strand, within GFF893 at 73.902 kb on - strand, within GFF893 at 74.194 kb on - strand, within GFF893 at 74.418 kb on + strand, within GFF893 at 74.433 kb on - strand, within GFF893 at 74.532 kb on + strand at 74.541 kb on - strand at 74.541 kb on - strand at 74.618 kb on + strand at 74.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 71,396 - GFF891 0.14 -1.6 71,400 - GFF891 0.14 -0.6 71,450 - GFF891 0.19 -0.8 71,525 - GFF891 0.26 +0.2 71,765 - GFF891 0.50 -0.4 71,765 - GFF891 0.50 -0.2 71,768 + GFF891 0.50 -0.9 71,769 - GFF891 0.51 +0.1 72,047 - GFF891 0.78 +0.2 72,082 + GFF891 0.82 +0.0 72,083 - GFF891 0.82 +0.3 72,170 + -0.5 72,462 + +1.2 72,463 - -0.1 72,663 - GFF892 0.22 -0.1 72,663 - GFF892 0.22 +0.6 72,811 + GFF892 0.33 +0.6 72,820 + GFF892 0.34 -0.5 72,893 + GFF892 0.39 -1.1 72,894 - GFF892 0.39 -0.4 72,986 + GFF892 0.46 +1.9 72,986 + GFF892 0.46 -1.0 72,987 - GFF892 0.46 -0.8 72,988 + GFF892 0.46 -0.1 72,989 - GFF892 0.46 -0.6 73,070 + GFF892 0.52 +0.4 73,172 + GFF892 0.60 -0.2 73,197 + GFF892 0.62 +0.3 73,297 + GFF892 0.69 +0.6 73,466 + GFF892 0.82 -0.8 73,467 - GFF892 0.82 -0.7 73,467 - GFF892 0.82 -1.1 73,491 - GFF892 0.84 +0.0 73,592 + +0.3 73,592 + +0.2 73,592 + +0.4 73,592 + -1.2 73,640 - -0.2 73,678 + +0.7 73,679 - -0.6 73,687 - +1.2 73,799 + GFF893 0.11 -0.4 73,819 + GFF893 0.13 +0.7 73,820 - GFF893 0.13 +0.2 73,820 - GFF893 0.13 -0.2 73,846 + GFF893 0.16 +0.0 73,852 - GFF893 0.17 -0.5 73,888 + GFF893 0.21 +0.2 73,889 - GFF893 0.22 +0.2 73,902 - GFF893 0.23 -2.2 74,194 - GFF893 0.58 -0.2 74,418 + GFF893 0.85 -0.2 74,433 - GFF893 0.87 +0.1 74,532 + -1.0 74,541 - +0.7 74,541 - +0.2 74,618 + -0.6 74,647 + +0.2
Or see this region's nucleotide sequence