Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF841 and GFF842 are separated by 44 nucleotides GFF842 and GFF843 are separated by 130 nucleotides GFF843 and GFF844 are separated by 210 nucleotides GFF844 and GFF845 overlap by 4 nucleotides GFF845 and GFF846 are separated by 104 nucleotides
GFF841 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 22,963 to 24,585
GFF841
GFF842 - Putative uncharacterized protein YjfN, at 24,630 to 24,905
GFF842
GFF843 - FIG01200701: possible membrane protein, at 25,036 to 25,338
GFF843
GFF844 - YjfP protein, at 25,549 to 26,298
GFF844
GFF845 - Ascorbate utilization transcriptional regulator UlaR, HTH-type, at 26,295 to 27,050
GFF845
GFF846 - Probable L-ascorbate-6-phosphate lactonase UlaG (EC 3.1.1.-) (L-ascorbate utilization protein G), at 27,155 to 28,219
GFF846
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.555 kb on - strand at 24.598 kb on - strand at 24.600 kb on + strand at 24.600 kb on + strand at 24.637 kb on + strand at 24.682 kb on + strand, within GFF842 at 24.682 kb on + strand, within GFF842 at 24.683 kb on - strand, within GFF842 at 24.917 kb on + strand at 24.920 kb on + strand at 24.957 kb on - strand at 25.104 kb on + strand, within GFF843 at 25.153 kb on + strand, within GFF843 at 25.154 kb on - strand, within GFF843 at 25.154 kb on - strand, within GFF843 at 25.189 kb on + strand, within GFF843 at 25.304 kb on - strand, within GFF843 at 25.304 kb on - strand, within GFF843 at 25.316 kb on - strand at 25.339 kb on - strand at 25.339 kb on - strand at 25.345 kb on - strand at 25.371 kb on - strand at 25.371 kb on - strand at 25.378 kb on + strand at 25.415 kb on + strand at 25.416 kb on - strand at 25.482 kb on + strand at 25.512 kb on - strand at 25.554 kb on - strand at 25.575 kb on - strand at 25.590 kb on - strand at 25.592 kb on + strand at 25.592 kb on + strand at 25.592 kb on + strand at 25.593 kb on - strand at 25.593 kb on - strand at 25.646 kb on - strand, within GFF844 at 25.646 kb on - strand, within GFF844 at 25.677 kb on + strand, within GFF844 at 25.677 kb on + strand, within GFF844 at 25.678 kb on - strand, within GFF844 at 25.830 kb on - strand, within GFF844 at 25.830 kb on - strand, within GFF844 at 25.830 kb on - strand, within GFF844 at 25.920 kb on + strand, within GFF844 at 25.921 kb on - strand, within GFF844 at 25.921 kb on - strand, within GFF844 at 25.921 kb on - strand, within GFF844 at 25.921 kb on - strand, within GFF844 at 26.050 kb on - strand, within GFF844 at 26.064 kb on - strand, within GFF844 at 26.247 kb on + strand at 26.308 kb on - strand at 26.432 kb on + strand, within GFF845 at 26.563 kb on + strand, within GFF845 at 26.613 kb on - strand, within GFF845 at 26.613 kb on - strand, within GFF845 at 26.633 kb on + strand, within GFF845 at 26.634 kb on - strand, within GFF845 at 26.665 kb on + strand, within GFF845 at 26.754 kb on - strand, within GFF845
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 24,555 - -1.0 24,598 - -0.7 24,600 + -0.0 24,600 + -0.0 24,637 + -0.2 24,682 + GFF842 0.19 +0.6 24,682 + GFF842 0.19 -0.7 24,683 - GFF842 0.19 -0.2 24,917 + +0.3 24,920 + -0.4 24,957 - -1.0 25,104 + GFF843 0.22 +0.5 25,153 + GFF843 0.39 -0.2 25,154 - GFF843 0.39 -0.8 25,154 - GFF843 0.39 -0.1 25,189 + GFF843 0.50 +0.1 25,304 - GFF843 0.88 +0.5 25,304 - GFF843 0.88 +0.4 25,316 - -2.4 25,339 - +0.2 25,339 - +0.1 25,345 - +0.2 25,371 - +0.2 25,371 - +0.1 25,378 + +0.0 25,415 + +0.3 25,416 - -0.7 25,482 + -0.1 25,512 - -0.2 25,554 - +0.1 25,575 - -0.7 25,590 - -1.9 25,592 + -0.3 25,592 + -0.7 25,592 + +0.2 25,593 - +0.1 25,593 - -0.2 25,646 - GFF844 0.13 -0.1 25,646 - GFF844 0.13 +0.5 25,677 + GFF844 0.17 +0.6 25,677 + GFF844 0.17 +0.2 25,678 - GFF844 0.17 +0.5 25,830 - GFF844 0.37 -0.3 25,830 - GFF844 0.37 +0.5 25,830 - GFF844 0.37 -0.0 25,920 + GFF844 0.49 +0.2 25,921 - GFF844 0.50 +0.2 25,921 - GFF844 0.50 +0.3 25,921 - GFF844 0.50 -0.1 25,921 - GFF844 0.50 +0.1 26,050 - GFF844 0.67 +0.0 26,064 - GFF844 0.69 +0.1 26,247 + -0.1 26,308 - -0.6 26,432 + GFF845 0.18 -0.6 26,563 + GFF845 0.35 -1.0 26,613 - GFF845 0.42 -0.6 26,613 - GFF845 0.42 -0.2 26,633 + GFF845 0.45 -1.4 26,634 - GFF845 0.45 -0.3 26,665 + GFF845 0.49 -0.5 26,754 - GFF845 0.61 -0.2
Or see this region's nucleotide sequence