Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF805 and GFF806 are separated by 3 nucleotides GFF806 and GFF807 overlap by 4 nucleotides GFF807 and GFF808 are separated by 10 nucleotides GFF808 and GFF809 are separated by 44 nucleotides GFF809 and GFF810 are separated by 20 nucleotides GFF810 and GFF811 overlap by 29 nucleotides
GFF805 - Phage protein, at 152,918 to 153,193
GFF805
GFF806 - Phage tail completion protein, at 153,197 to 153,781
GFF806
GFF807 - Phage minor tail protein, at 153,778 to 154,179
GFF807
GFF808 - Phage major tail protein, at 154,190 to 154,933
GFF808
GFF809 - Phage minor tail protein, at 154,978 to 155,382
GFF809
GFF810 - Phage minor tail protein, at 155,403 to 155,717
GFF810
GFF811 - Phage tail length tape-measure protein 1, at 155,689 to 158,850
GFF811
Position (kb) 154
155 Strain fitness (log2 ratio) -1
0
1 at 153.253 kb on + strand at 153.554 kb on + strand, within GFF806 at 153.574 kb on + strand, within GFF806 at 153.608 kb on - strand, within GFF806 at 153.706 kb on + strand, within GFF806 at 153.741 kb on - strand at 154.017 kb on + strand, within GFF807 at 154.078 kb on - strand, within GFF807 at 154.083 kb on + strand, within GFF807 at 154.109 kb on - strand, within GFF807 at 154.169 kb on - strand at 154.183 kb on + strand at 154.312 kb on + strand, within GFF808 at 154.364 kb on + strand, within GFF808 at 154.365 kb on - strand, within GFF808 at 154.365 kb on - strand, within GFF808 at 154.741 kb on + strand, within GFF808 at 155.001 kb on + strand at 155.001 kb on + strand at 155.006 kb on - strand at 155.006 kb on - strand at 155.071 kb on - strand, within GFF809 at 155.142 kb on + strand, within GFF809 at 155.226 kb on - strand, within GFF809 at 155.228 kb on - strand, within GFF809 at 155.343 kb on + strand at 155.609 kb on - strand, within GFF810 at 155.790 kb on + strand at 155.932 kb on + strand at 155.932 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 153,253 + -0.3 153,554 + GFF806 0.61 +0.3 153,574 + GFF806 0.64 +0.2 153,608 - GFF806 0.70 +0.5 153,706 + GFF806 0.87 +0.0 153,741 - -0.7 154,017 + GFF807 0.59 +0.1 154,078 - GFF807 0.75 -0.8 154,083 + GFF807 0.76 -0.5 154,109 - GFF807 0.82 +0.1 154,169 - +0.0 154,183 + +0.5 154,312 + GFF808 0.16 +0.5 154,364 + GFF808 0.23 -0.2 154,365 - GFF808 0.24 +0.3 154,365 - GFF808 0.24 -0.3 154,741 + GFF808 0.74 +0.3 155,001 + -0.0 155,001 + +0.5 155,006 - +0.1 155,006 - +0.1 155,071 - GFF809 0.23 +0.2 155,142 + GFF809 0.40 -0.9 155,226 - GFF809 0.61 -0.0 155,228 - GFF809 0.62 +0.2 155,343 + -0.6 155,609 - GFF810 0.65 +0.8 155,790 + -0.3 155,932 + +1.5 155,932 + +0.9
Or see this region's nucleotide sequence