Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF730 and GFF731 are separated by 90 nucleotides GFF731 and GFF732 are separated by 26 nucleotides GFF732 and GFF733 are separated by 69 nucleotides
GFF730 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 89,591 to 90,502
GFF730
GFF731 - Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase, at 90,593 to 91,918
GFF731
GFF732 - hypothetical protein, at 91,945 to 92,079
GFF732
GFF733 - biofilm regulator BssR, at 92,149 to 92,532
GFF733
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-2
-1
0
1 at 89.643 kb on + strand at 89.975 kb on + strand, within GFF730 at 89.975 kb on + strand, within GFF730 at 89.998 kb on + strand, within GFF730 at 90.148 kb on + strand, within GFF730 at 90.148 kb on + strand, within GFF730 at 90.176 kb on - strand, within GFF730 at 90.230 kb on - strand, within GFF730 at 90.231 kb on + strand, within GFF730 at 90.232 kb on - strand, within GFF730 at 90.232 kb on - strand, within GFF730 at 90.687 kb on + strand at 90.724 kb on + strand at 90.830 kb on - strand, within GFF731 at 91.020 kb on - strand, within GFF731 at 91.054 kb on + strand, within GFF731 at 91.210 kb on - strand, within GFF731 at 91.317 kb on - strand, within GFF731 at 91.333 kb on + strand, within GFF731 at 91.334 kb on - strand, within GFF731 at 91.395 kb on - strand, within GFF731 at 91.497 kb on + strand, within GFF731 at 91.517 kb on + strand, within GFF731 at 91.580 kb on + strand, within GFF731 at 91.602 kb on + strand, within GFF731 at 91.603 kb on - strand, within GFF731 at 91.765 kb on + strand, within GFF731 at 91.766 kb on - strand, within GFF731 at 92.148 kb on + strand at 92.218 kb on + strand, within GFF733 at 92.245 kb on + strand, within GFF733 at 92.247 kb on + strand, within GFF733 at 92.247 kb on + strand, within GFF733 at 92.248 kb on - strand, within GFF733 at 92.248 kb on - strand, within GFF733 at 92.787 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 89,643 + +0.2 89,975 + GFF730 0.42 +1.0 89,975 + GFF730 0.42 -0.2 89,998 + GFF730 0.45 +0.4 90,148 + GFF730 0.61 +0.3 90,148 + GFF730 0.61 +0.7 90,176 - GFF730 0.64 -0.1 90,230 - GFF730 0.70 +0.1 90,231 + GFF730 0.70 +0.3 90,232 - GFF730 0.70 +0.4 90,232 - GFF730 0.70 -1.8 90,687 + -0.2 90,724 + -0.1 90,830 - GFF731 0.18 +0.1 91,020 - GFF731 0.32 +0.6 91,054 + GFF731 0.35 -0.3 91,210 - GFF731 0.47 -0.8 91,317 - GFF731 0.55 +0.2 91,333 + GFF731 0.56 -1.0 91,334 - GFF731 0.56 -0.1 91,395 - GFF731 0.60 -0.2 91,497 + GFF731 0.68 -0.7 91,517 + GFF731 0.70 +0.0 91,580 + GFF731 0.74 -1.0 91,602 + GFF731 0.76 -0.6 91,603 - GFF731 0.76 -0.5 91,765 + GFF731 0.88 -1.1 91,766 - GFF731 0.88 -0.3 92,148 + -0.3 92,218 + GFF733 0.18 +0.5 92,245 + GFF733 0.25 -0.0 92,247 + GFF733 0.26 +0.4 92,247 + GFF733 0.26 +1.0 92,248 - GFF733 0.26 -0.4 92,248 - GFF733 0.26 +1.2 92,787 - +0.5
Or see this region's nucleotide sequence