Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF664 and GFF665 are separated by 44 nucleotides GFF665 and GFF666 are separated by 196 nucleotides GFF666 and GFF667 overlap by 1 nucleotides GFF667 and GFF668 are separated by 1 nucleotides
GFF664 - Formiminoglutamase (EC 3.5.3.8), at 20,125 to 21,066
GFF664
GFF665 - Histidine utilization repressor, at 21,111 to 21,836
GFF665
GFF666 - Urocanate hydratase (EC 4.2.1.49), at 22,033 to 23,178
GFF666
GFF667 - Urocanate hydratase (EC 4.2.1.49), at 23,178 to 23,717
GFF667
GFF668 - Histidine ammonia-lyase (EC 4.3.1.3), at 23,719 to 25,239
GFF668
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-1
0
1 at 21.114 kb on + strand at 21.114 kb on + strand at 21.115 kb on - strand at 21.316 kb on + strand, within GFF665 at 21.522 kb on + strand, within GFF665 at 21.600 kb on + strand, within GFF665 at 21.663 kb on - strand, within GFF665 at 21.710 kb on + strand, within GFF665 at 21.787 kb on + strand at 21.827 kb on + strand at 21.935 kb on - strand at 22.015 kb on + strand at 22.016 kb on - strand at 22.048 kb on + strand at 22.130 kb on + strand at 22.184 kb on - strand, within GFF666 at 22.415 kb on - strand, within GFF666 at 22.445 kb on - strand, within GFF666 at 22.446 kb on + strand, within GFF666 at 22.477 kb on + strand, within GFF666 at 22.477 kb on + strand, within GFF666 at 22.511 kb on - strand, within GFF666 at 22.653 kb on + strand, within GFF666 at 22.708 kb on - strand, within GFF666 at 22.841 kb on - strand, within GFF666 at 22.848 kb on + strand, within GFF666 at 22.849 kb on - strand, within GFF666 at 22.853 kb on - strand, within GFF666 at 22.996 kb on - strand, within GFF666 at 23.073 kb on + strand at 23.073 kb on + strand at 23.144 kb on + strand at 23.144 kb on + strand at 23.177 kb on - strand at 23.177 kb on - strand at 23.189 kb on + strand at 23.267 kb on - strand, within GFF667 at 23.594 kb on - strand, within GFF667 at 23.719 kb on - strand at 23.751 kb on - strand at 23.751 kb on - strand at 23.772 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 21,114 + -0.6 21,114 + +0.1 21,115 - -0.7 21,316 + GFF665 0.28 -0.1 21,522 + GFF665 0.57 -0.0 21,600 + GFF665 0.67 +0.1 21,663 - GFF665 0.76 +0.1 21,710 + GFF665 0.83 -0.1 21,787 + -0.0 21,827 + +0.4 21,935 - -1.2 22,015 + +0.4 22,016 - +0.4 22,048 + -0.9 22,130 + -0.7 22,184 - GFF666 0.13 -0.6 22,415 - GFF666 0.33 -1.0 22,445 - GFF666 0.36 -0.4 22,446 + GFF666 0.36 -0.1 22,477 + GFF666 0.39 -0.5 22,477 + GFF666 0.39 +1.1 22,511 - GFF666 0.42 +0.2 22,653 + GFF666 0.54 -0.1 22,708 - GFF666 0.59 -0.2 22,841 - GFF666 0.71 +0.4 22,848 + GFF666 0.71 -0.7 22,849 - GFF666 0.71 +0.2 22,853 - GFF666 0.72 -0.3 22,996 - GFF666 0.84 -0.5 23,073 + +0.2 23,073 + +0.2 23,144 + +0.2 23,144 + -0.9 23,177 - -0.0 23,177 - -0.6 23,189 + -0.0 23,267 - GFF667 0.16 -0.8 23,594 - GFF667 0.77 -0.2 23,719 - -0.1 23,751 - -0.1 23,751 - -0.6 23,772 + -1.0
Or see this region's nucleotide sequence