Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF652 and GFF653 are separated by 222 nucleotides GFF653 and GFF654 are separated by 170 nucleotides
GFF652 - UDP-N-acetylglucosamine 4-epimerase (EC 5.1.3.7) / UDP-glucose 4-epimerase (EC 5.1.3.2), at 7,645 to 8,661
GFF652
GFF653 - Putative inner membrane protein, at 8,884 to 9,792
GFF653
GFF654 - Putative molybdenum transport ATP-binding protein modF, at 9,963 to 11,438
GFF654
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 7.884 kb on + strand, within GFF652 at 7.886 kb on + strand, within GFF652 at 7.887 kb on - strand, within GFF652 at 7.890 kb on + strand, within GFF652 at 7.890 kb on + strand, within GFF652 at 8.053 kb on - strand, within GFF652 at 8.054 kb on + strand, within GFF652 at 8.054 kb on + strand, within GFF652 at 8.055 kb on - strand, within GFF652 at 8.206 kb on - strand, within GFF652 at 8.298 kb on + strand, within GFF652 at 8.313 kb on + strand, within GFF652 at 8.680 kb on + strand at 8.681 kb on - strand at 8.686 kb on - strand at 8.693 kb on - strand at 8.698 kb on + strand at 8.985 kb on + strand, within GFF653 at 8.986 kb on - strand, within GFF653 at 9.041 kb on + strand, within GFF653 at 9.042 kb on - strand, within GFF653 at 9.203 kb on + strand, within GFF653 at 9.212 kb on + strand, within GFF653 at 9.303 kb on + strand, within GFF653 at 9.439 kb on + strand, within GFF653 at 9.597 kb on + strand, within GFF653 at 9.617 kb on - strand, within GFF653 at 10.031 kb on + strand at 10.098 kb on + strand at 10.101 kb on + strand at 10.102 kb on - strand at 10.107 kb on + strand at 10.187 kb on + strand, within GFF654 at 10.260 kb on - strand, within GFF654 at 10.340 kb on + strand, within GFF654 at 10.374 kb on + strand, within GFF654 at 10.484 kb on + strand, within GFF654 at 10.484 kb on + strand, within GFF654 at 10.499 kb on - strand, within GFF654 at 10.520 kb on + strand, within GFF654 at 10.529 kb on + strand, within GFF654 at 10.557 kb on - strand, within GFF654 at 10.602 kb on + strand, within GFF654 at 10.603 kb on - strand, within GFF654 at 10.630 kb on + strand, within GFF654 at 10.649 kb on + strand, within GFF654 at 10.720 kb on - strand, within GFF654
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 7,884 + GFF652 0.24 -1.6 7,886 + GFF652 0.24 -1.5 7,887 - GFF652 0.24 -3.9 7,890 + GFF652 0.24 -2.0 7,890 + GFF652 0.24 -1.0 8,053 - GFF652 0.40 -1.2 8,054 + GFF652 0.40 -2.3 8,054 + GFF652 0.40 -3.0 8,055 - GFF652 0.40 -2.3 8,206 - GFF652 0.55 -1.7 8,298 + GFF652 0.64 -3.1 8,313 + GFF652 0.66 -3.6 8,680 + -1.3 8,681 - -1.4 8,686 - -1.3 8,693 - -0.6 8,698 + -1.9 8,985 + GFF653 0.11 -0.5 8,986 - GFF653 0.11 -0.9 9,041 + GFF653 0.17 +0.8 9,042 - GFF653 0.17 +0.4 9,203 + GFF653 0.35 -1.1 9,212 + GFF653 0.36 -0.1 9,303 + GFF653 0.46 -0.5 9,439 + GFF653 0.61 -0.7 9,597 + GFF653 0.78 +0.0 9,617 - GFF653 0.81 -0.2 10,031 + -0.1 10,098 + +0.1 10,101 + -1.2 10,102 - +0.2 10,107 + -1.3 10,187 + GFF654 0.15 -1.3 10,260 - GFF654 0.20 +0.5 10,340 + GFF654 0.26 -0.3 10,374 + GFF654 0.28 -0.0 10,484 + GFF654 0.35 -0.3 10,484 + GFF654 0.35 -0.1 10,499 - GFF654 0.36 +0.0 10,520 + GFF654 0.38 +0.5 10,529 + GFF654 0.38 -0.1 10,557 - GFF654 0.40 +0.3 10,602 + GFF654 0.43 -0.0 10,603 - GFF654 0.43 +0.7 10,630 + GFF654 0.45 -0.5 10,649 + GFF654 0.46 -0.1 10,720 - GFF654 0.51 +0.3
Or see this region's nucleotide sequence