Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF648 and GFF649 are separated by 223 nucleotides GFF649 and GFF650 overlap by 7 nucleotides
GFF648 - Phosphoglycerate mutase (EC 5.4.2.1), at 3,427 to 4,179
GFF648
GFF649 - Aldose 1-epimerase (EC 5.1.3.3), at 4,403 to 5,443
GFF649
GFF650 - Galactokinase (EC 2.7.1.6), at 5,437 to 6,585
GFF650
Position (kb) 4
5
6 Strain fitness (log2 ratio) -1
0
1 at 3.404 kb on - strand at 3.404 kb on - strand at 3.404 kb on - strand at 3.497 kb on + strand at 3.498 kb on - strand at 3.498 kb on - strand at 3.533 kb on + strand, within GFF648 at 3.533 kb on + strand, within GFF648 at 3.592 kb on - strand, within GFF648 at 3.970 kb on + strand, within GFF648 at 3.971 kb on - strand, within GFF648 at 4.103 kb on + strand, within GFF648 at 4.172 kb on - strand at 4.192 kb on - strand at 4.194 kb on - strand at 4.194 kb on - strand at 4.194 kb on - strand at 4.404 kb on + strand at 4.441 kb on - strand at 4.462 kb on + strand at 4.621 kb on - strand, within GFF649 at 4.874 kb on - strand, within GFF649 at 4.874 kb on - strand, within GFF649 at 4.915 kb on - strand, within GFF649 at 4.981 kb on + strand, within GFF649 at 4.981 kb on + strand, within GFF649 at 4.981 kb on + strand, within GFF649 at 4.982 kb on - strand, within GFF649 at 5.188 kb on + strand, within GFF649 at 5.191 kb on - strand, within GFF649 at 5.470 kb on + strand at 5.471 kb on - strand at 5.471 kb on - strand at 5.474 kb on + strand at 5.650 kb on + strand, within GFF650 at 5.719 kb on - strand, within GFF650 at 5.783 kb on - strand, within GFF650 at 5.783 kb on - strand, within GFF650 at 5.783 kb on - strand, within GFF650 at 5.913 kb on + strand, within GFF650 at 5.916 kb on + strand, within GFF650 at 5.916 kb on + strand, within GFF650 at 5.917 kb on - strand, within GFF650 at 5.919 kb on - strand, within GFF650 at 6.395 kb on - strand, within GFF650 at 6.423 kb on + strand, within GFF650
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 3,404 - +0.2 3,404 - +0.3 3,404 - +0.2 3,497 + +0.1 3,498 - -0.4 3,498 - +0.2 3,533 + GFF648 0.14 +0.3 3,533 + GFF648 0.14 +0.9 3,592 - GFF648 0.22 +0.0 3,970 + GFF648 0.72 +0.8 3,971 - GFF648 0.72 -0.2 4,103 + GFF648 0.90 -0.3 4,172 - -0.2 4,192 - -0.1 4,194 - -0.1 4,194 - +0.2 4,194 - -0.7 4,404 + +0.4 4,441 - -0.7 4,462 + -1.1 4,621 - GFF649 0.21 +0.1 4,874 - GFF649 0.45 -0.9 4,874 - GFF649 0.45 -0.2 4,915 - GFF649 0.49 +0.2 4,981 + GFF649 0.56 +0.1 4,981 + GFF649 0.56 -1.5 4,981 + GFF649 0.56 -0.3 4,982 - GFF649 0.56 -0.1 5,188 + GFF649 0.75 -1.0 5,191 - GFF649 0.76 -0.3 5,470 + -1.5 5,471 - +0.2 5,471 - +0.3 5,474 + -0.1 5,650 + GFF650 0.19 -1.2 5,719 - GFF650 0.25 -0.1 5,783 - GFF650 0.30 -0.7 5,783 - GFF650 0.30 +0.3 5,783 - GFF650 0.30 +0.3 5,913 + GFF650 0.41 -0.3 5,916 + GFF650 0.42 -0.5 5,916 + GFF650 0.42 -0.5 5,917 - GFF650 0.42 -0.2 5,919 - GFF650 0.42 -0.2 6,395 - GFF650 0.83 +0.4 6,423 + GFF650 0.86 -0.5
Or see this region's nucleotide sequence