Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF603 and GFF604 are separated by 58 nucleotides GFF604 and GFF605 are separated by 85 nucleotides GFF605 and GFF606 are separated by 35 nucleotides
GFF603 - NfuA Fe-S protein maturation, at 343,066 to 343,641
GFF603
GFF604 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 343,700 to 344,383
GFF604
GFF605 - Biotin synthesis protein BioH, at 344,469 to 345,191
GFF605
GFF606 - Uncharacterized protein YfaD, at 345,227 to 346,141
GFF606
Position (kb) 344
345
346 Strain fitness (log2 ratio) -2
-1
0
1 at 343.514 kb on + strand, within GFF603 at 343.514 kb on + strand, within GFF603 at 343.633 kb on - strand at 343.655 kb on + strand at 343.679 kb on - strand at 343.702 kb on - strand at 343.781 kb on - strand, within GFF604 at 343.811 kb on - strand, within GFF604 at 343.874 kb on + strand, within GFF604 at 343.990 kb on + strand, within GFF604 at 344.157 kb on - strand, within GFF604 at 344.173 kb on + strand, within GFF604 at 344.173 kb on + strand, within GFF604 at 344.174 kb on - strand, within GFF604 at 344.174 kb on - strand, within GFF604 at 344.176 kb on - strand, within GFF604 at 344.333 kb on + strand at 344.333 kb on + strand at 344.363 kb on + strand at 344.379 kb on - strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.383 kb on + strand at 344.384 kb on - strand at 344.384 kb on - strand at 344.415 kb on + strand at 344.415 kb on + strand at 344.415 kb on + strand at 344.416 kb on - strand at 344.446 kb on - strand at 344.504 kb on - strand at 344.540 kb on + strand at 344.938 kb on + strand, within GFF605 at 344.939 kb on - strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.027 kb on + strand, within GFF605 at 345.028 kb on - strand, within GFF605 at 345.028 kb on - strand, within GFF605 at 345.028 kb on - strand, within GFF605 at 345.106 kb on + strand, within GFF605 at 345.134 kb on + strand at 345.274 kb on + strand at 345.274 kb on + strand at 345.374 kb on - strand, within GFF606 at 345.392 kb on + strand, within GFF606 at 345.392 kb on + strand, within GFF606 at 345.406 kb on + strand, within GFF606 at 345.436 kb on + strand, within GFF606 at 345.437 kb on - strand, within GFF606 at 345.463 kb on + strand, within GFF606 at 345.498 kb on + strand, within GFF606 at 345.694 kb on - strand, within GFF606 at 345.696 kb on - strand, within GFF606 at 345.701 kb on - strand, within GFF606 at 345.808 kb on + strand, within GFF606 at 345.872 kb on - strand, within GFF606 at 345.872 kb on - strand, within GFF606 at 345.886 kb on + strand, within GFF606 at 345.909 kb on + strand, within GFF606 at 345.915 kb on + strand, within GFF606
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 343,514 + GFF603 0.78 -1.9 343,514 + GFF603 0.78 -0.8 343,633 - -1.0 343,655 + +0.4 343,679 - +0.3 343,702 - +0.2 343,781 - GFF604 0.12 +0.2 343,811 - GFF604 0.16 -0.4 343,874 + GFF604 0.25 -0.6 343,990 + GFF604 0.42 +0.6 344,157 - GFF604 0.67 +0.2 344,173 + GFF604 0.69 -1.4 344,173 + GFF604 0.69 -0.0 344,174 - GFF604 0.69 -0.1 344,174 - GFF604 0.69 +0.5 344,176 - GFF604 0.70 +0.1 344,333 + +0.1 344,333 + -0.1 344,363 + -0.4 344,379 - +0.7 344,383 + -0.6 344,383 + +0.0 344,383 + +0.0 344,383 + -0.0 344,383 + +0.4 344,383 + -0.3 344,383 + +0.1 344,384 - +0.3 344,384 - -0.9 344,415 + +0.4 344,415 + +0.3 344,415 + -0.0 344,416 - +0.8 344,446 - -0.1 344,504 - -0.3 344,540 + -0.1 344,938 + GFF605 0.65 +0.0 344,939 - GFF605 0.65 +1.2 345,027 + GFF605 0.77 +0.5 345,027 + GFF605 0.77 -1.0 345,027 + GFF605 0.77 +0.4 345,027 + GFF605 0.77 +0.2 345,028 - GFF605 0.77 -1.4 345,028 - GFF605 0.77 -0.1 345,028 - GFF605 0.77 -0.4 345,106 + GFF605 0.88 -0.2 345,134 + -0.3 345,274 + +0.8 345,274 + -0.1 345,374 - GFF606 0.16 +0.2 345,392 + GFF606 0.18 +1.2 345,392 + GFF606 0.18 -0.0 345,406 + GFF606 0.20 -0.0 345,436 + GFF606 0.23 -1.7 345,437 - GFF606 0.23 -0.2 345,463 + GFF606 0.26 +0.2 345,498 + GFF606 0.30 -0.1 345,694 - GFF606 0.51 -0.5 345,696 - GFF606 0.51 +0.4 345,701 - GFF606 0.52 +0.1 345,808 + GFF606 0.63 -0.2 345,872 - GFF606 0.70 +0.2 345,872 - GFF606 0.70 +0.4 345,886 + GFF606 0.72 -1.3 345,909 + GFF606 0.75 -1.5 345,915 + GFF606 0.75 -0.1
Or see this region's nucleotide sequence