Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF584 and GFF585 are separated by 192 nucleotides GFF585 and GFF586 are separated by 9 nucleotides GFF586 and GFF587 are separated by 46 nucleotides
GFF584 - Glycerol dehydrogenase (EC 1.1.1.6), at 319,476 to 320,585
GFF584
GFF585 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 320,778 to 322,238
GFF585
GFF586 - Putative inner membrane protein, at 322,248 to 322,793
GFF586
GFF587 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 322,840 to 324,348
GFF587
Position (kb) 320
321
322
323 Strain fitness (log2 ratio) -1
0
1
2 at 319.820 kb on + strand, within GFF584 at 319.862 kb on + strand, within GFF584 at 319.867 kb on - strand, within GFF584 at 319.893 kb on + strand, within GFF584 at 319.911 kb on - strand, within GFF584 at 319.940 kb on + strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 319.941 kb on - strand, within GFF584 at 320.094 kb on + strand, within GFF584 at 320.105 kb on - strand, within GFF584 at 320.105 kb on - strand, within GFF584 at 320.112 kb on + strand, within GFF584 at 320.187 kb on - strand, within GFF584 at 320.187 kb on - strand, within GFF584 at 320.192 kb on - strand, within GFF584 at 320.280 kb on + strand, within GFF584 at 320.324 kb on + strand, within GFF584 at 320.325 kb on - strand, within GFF584 at 320.325 kb on - strand, within GFF584 at 320.383 kb on + strand, within GFF584 at 320.383 kb on + strand, within GFF584 at 320.705 kb on + strand at 320.707 kb on + strand at 320.708 kb on - strand at 320.776 kb on + strand at 320.813 kb on + strand at 320.814 kb on - strand at 320.844 kb on + strand at 320.844 kb on + strand at 321.012 kb on - strand, within GFF585 at 321.012 kb on - strand, within GFF585 at 321.068 kb on + strand, within GFF585 at 321.069 kb on - strand, within GFF585 at 321.090 kb on - strand, within GFF585 at 321.124 kb on + strand, within GFF585 at 321.137 kb on - strand, within GFF585 at 321.213 kb on + strand, within GFF585 at 321.214 kb on - strand, within GFF585 at 321.313 kb on - strand, within GFF585 at 321.328 kb on - strand, within GFF585 at 321.364 kb on - strand, within GFF585 at 321.549 kb on + strand, within GFF585 at 321.611 kb on + strand, within GFF585 at 321.667 kb on + strand, within GFF585 at 321.679 kb on - strand, within GFF585 at 321.710 kb on + strand, within GFF585 at 321.822 kb on - strand, within GFF585 at 321.877 kb on + strand, within GFF585 at 322.002 kb on + strand, within GFF585 at 322.002 kb on + strand, within GFF585 at 322.034 kb on + strand, within GFF585 at 322.034 kb on + strand, within GFF585 at 322.092 kb on + strand, within GFF585 at 322.099 kb on - strand at 322.114 kb on - strand at 322.115 kb on + strand at 322.116 kb on - strand at 322.209 kb on + strand at 322.210 kb on - strand at 322.210 kb on - strand at 322.343 kb on + strand, within GFF586 at 322.349 kb on + strand, within GFF586 at 322.349 kb on + strand, within GFF586 at 322.438 kb on + strand, within GFF586 at 322.642 kb on - strand, within GFF586 at 322.670 kb on + strand, within GFF586 at 322.733 kb on - strand, within GFF586 at 322.751 kb on + strand at 322.752 kb on - strand at 322.763 kb on + strand at 322.763 kb on + strand at 322.800 kb on + strand at 322.801 kb on - strand at 322.801 kb on - strand at 322.822 kb on - strand at 322.842 kb on - strand at 322.950 kb on + strand at 323.100 kb on - strand, within GFF587
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 319,820 + GFF584 0.31 +1.8 319,862 + GFF584 0.35 -0.6 319,867 - GFF584 0.35 +0.0 319,893 + GFF584 0.38 +0.1 319,911 - GFF584 0.39 +0.5 319,940 + GFF584 0.42 +0.1 319,941 - GFF584 0.42 -0.2 319,941 - GFF584 0.42 +0.3 319,941 - GFF584 0.42 +0.5 320,094 + GFF584 0.56 -0.6 320,105 - GFF584 0.57 -0.0 320,105 - GFF584 0.57 -0.2 320,112 + GFF584 0.57 +0.2 320,187 - GFF584 0.64 +0.0 320,187 - GFF584 0.64 -0.2 320,192 - GFF584 0.65 -0.1 320,280 + GFF584 0.72 -0.9 320,324 + GFF584 0.76 -0.3 320,325 - GFF584 0.76 +0.2 320,325 - GFF584 0.76 -0.5 320,383 + GFF584 0.82 +0.4 320,383 + GFF584 0.82 -0.6 320,705 + -0.5 320,707 + +0.0 320,708 - +0.2 320,776 + +0.4 320,813 + +0.1 320,814 - -0.5 320,844 + +0.4 320,844 + +0.6 321,012 - GFF585 0.16 -1.1 321,012 - GFF585 0.16 -0.6 321,068 + GFF585 0.20 +0.3 321,069 - GFF585 0.20 +0.5 321,090 - GFF585 0.21 -1.0 321,124 + GFF585 0.24 +0.8 321,137 - GFF585 0.25 -0.4 321,213 + GFF585 0.30 -0.4 321,214 - GFF585 0.30 -0.2 321,313 - GFF585 0.37 -0.3 321,328 - GFF585 0.38 -1.4 321,364 - GFF585 0.40 +0.0 321,549 + GFF585 0.53 -1.0 321,611 + GFF585 0.57 -0.1 321,667 + GFF585 0.61 +0.2 321,679 - GFF585 0.62 -1.6 321,710 + GFF585 0.64 -0.1 321,822 - GFF585 0.71 -0.1 321,877 + GFF585 0.75 +0.0 322,002 + GFF585 0.84 -0.1 322,002 + GFF585 0.84 -0.1 322,034 + GFF585 0.86 -0.5 322,034 + GFF585 0.86 +0.4 322,092 + GFF585 0.90 +0.0 322,099 - +0.2 322,114 - -0.1 322,115 + -0.0 322,116 - -0.5 322,209 + -0.3 322,210 - -0.2 322,210 - -0.5 322,343 + GFF586 0.17 +0.2 322,349 + GFF586 0.18 +0.1 322,349 + GFF586 0.18 -1.2 322,438 + GFF586 0.35 -0.5 322,642 - GFF586 0.72 +1.0 322,670 + GFF586 0.77 +0.2 322,733 - GFF586 0.89 +0.5 322,751 + -0.2 322,752 - +0.2 322,763 + -0.0 322,763 + -0.1 322,800 + -0.1 322,801 - +0.4 322,801 - -1.2 322,822 - +0.6 322,842 - +0.5 322,950 + +0.6 323,100 - GFF587 0.17 -1.0
Or see this region's nucleotide sequence