Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF570 and GFF571 are separated by 177 nucleotides GFF571 and GFF572 overlap by 4 nucleotides GFF572 and GFF573 are separated by 229 nucleotides GFF573 and GFF574 are separated by 29 nucleotides
GFF570 - Gluconate utilization system Gnt-I transcriptional repressor, at 299,958 to 300,911
GFF570
GFF571 - Gluconokinase (EC 2.7.1.12), at 301,089 to 301,583
GFF571
GFF572 - Low-affinity gluconate/H+ symporter GntU, at 301,580 to 302,920
GFF572
GFF573 - FIG01046423: hypothetical protein, at 303,150 to 303,314
GFF573
GFF574 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 303,344 to 304,450
GFF574
Position (kb) 301
302
303 Strain fitness (log2 ratio) -1
0
1 at 300.641 kb on + strand, within GFF570 at 300.878 kb on - strand at 300.928 kb on + strand at 300.929 kb on - strand at 301.033 kb on + strand at 301.191 kb on - strand, within GFF571 at 301.191 kb on - strand, within GFF571 at 301.246 kb on + strand, within GFF571 at 301.247 kb on - strand, within GFF571 at 301.565 kb on + strand at 301.575 kb on - strand at 301.886 kb on + strand, within GFF572 at 301.887 kb on - strand, within GFF572 at 302.189 kb on + strand, within GFF572 at 302.780 kb on - strand, within GFF572 at 302.838 kb on + strand at 302.864 kb on - strand at 302.970 kb on + strand at 302.970 kb on + strand at 302.971 kb on - strand at 302.981 kb on + strand at 303.109 kb on + strand at 303.110 kb on - strand at 303.114 kb on - strand at 303.123 kb on - strand at 303.275 kb on - strand, within GFF573 at 303.310 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 300,641 + GFF570 0.72 -0.3 300,878 - -0.2 300,928 + +1.0 300,929 - +0.1 301,033 + +0.2 301,191 - GFF571 0.21 -1.0 301,191 - GFF571 0.21 +0.2 301,246 + GFF571 0.32 +0.9 301,247 - GFF571 0.32 -0.3 301,565 + -0.2 301,575 - -0.1 301,886 + GFF572 0.23 +0.4 301,887 - GFF572 0.23 +0.2 302,189 + GFF572 0.45 +0.6 302,780 - GFF572 0.89 +0.1 302,838 + +0.3 302,864 - -0.2 302,970 + +0.4 302,970 + -0.4 302,971 - -0.8 302,981 + +0.3 303,109 + -0.6 303,110 - +0.6 303,114 - -0.1 303,123 - +0.1 303,275 - GFF573 0.76 +0.5 303,310 + -1.5
Or see this region's nucleotide sequence