Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF523 and GFF524 are separated by 82 nucleotides GFF524 and GFF525 are separated by 91 nucleotides GFF525 and GFF526 overlap by 4 nucleotides
GFF523 - NAD(FAD)-utilizing dehydrogenases, at 254,813 to 256,009
GFF523
GFF524 - hypothetical protein, at 256,092 to 256,223
GFF524
GFF525 - Putative membrane protein, at 256,315 to 257,382
GFF525
GFF526 - ABC-type multidrug transport system, permease component, at 257,379 to 260,120
GFF526
Position (kb) 256
257
258 Strain fitness (log2 ratio) -1
0
1
2 at 255.394 kb on + strand, within GFF523 at 255.395 kb on - strand, within GFF523 at 255.400 kb on - strand, within GFF523 at 255.404 kb on - strand, within GFF523 at 255.414 kb on + strand, within GFF523 at 255.549 kb on - strand, within GFF523 at 255.567 kb on + strand, within GFF523 at 255.567 kb on + strand, within GFF523 at 255.582 kb on + strand, within GFF523 at 255.696 kb on + strand, within GFF523 at 255.723 kb on + strand, within GFF523 at 255.796 kb on + strand, within GFF523 at 255.858 kb on + strand, within GFF523 at 255.947 kb on - strand at 256.035 kb on - strand at 256.057 kb on - strand at 256.057 kb on - strand at 256.059 kb on + strand at 256.067 kb on + strand at 256.067 kb on + strand at 256.076 kb on + strand at 256.127 kb on + strand, within GFF524 at 256.149 kb on + strand, within GFF524 at 256.149 kb on + strand, within GFF524 at 256.149 kb on + strand, within GFF524 at 256.150 kb on - strand, within GFF524 at 256.150 kb on - strand, within GFF524 at 256.763 kb on + strand, within GFF525 at 256.870 kb on - strand, within GFF525 at 256.875 kb on + strand, within GFF525 at 256.878 kb on + strand, within GFF525 at 256.956 kb on - strand, within GFF525 at 257.061 kb on + strand, within GFF525 at 257.062 kb on - strand, within GFF525 at 257.152 kb on - strand, within GFF525 at 257.290 kb on + strand at 257.360 kb on + strand at 257.469 kb on - strand at 257.493 kb on - strand at 257.493 kb on - strand at 257.679 kb on + strand, within GFF526 at 257.944 kb on + strand, within GFF526 at 257.944 kb on + strand, within GFF526 at 257.954 kb on + strand, within GFF526 at 258.000 kb on + strand, within GFF526 at 258.001 kb on - strand, within GFF526 at 258.002 kb on + strand, within GFF526 at 258.158 kb on + strand, within GFF526 at 258.180 kb on + strand, within GFF526
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 255,394 + GFF523 0.49 +0.1 255,395 - GFF523 0.49 +0.8 255,400 - GFF523 0.49 -0.5 255,404 - GFF523 0.49 -1.5 255,414 + GFF523 0.50 -0.1 255,549 - GFF523 0.61 +0.8 255,567 + GFF523 0.63 -0.4 255,567 + GFF523 0.63 +0.2 255,582 + GFF523 0.64 +0.2 255,696 + GFF523 0.74 +0.0 255,723 + GFF523 0.76 +0.6 255,796 + GFF523 0.82 -0.1 255,858 + GFF523 0.87 +0.5 255,947 - +0.2 256,035 - -0.2 256,057 - -0.8 256,057 - +0.2 256,059 + -0.0 256,067 + +0.5 256,067 + +0.3 256,076 + +0.1 256,127 + GFF524 0.27 +0.7 256,149 + GFF524 0.43 -0.8 256,149 + GFF524 0.43 -0.4 256,149 + GFF524 0.43 -1.3 256,150 - GFF524 0.44 +0.2 256,150 - GFF524 0.44 -0.3 256,763 + GFF525 0.42 +0.3 256,870 - GFF525 0.52 -0.8 256,875 + GFF525 0.52 +0.8 256,878 + GFF525 0.53 -0.4 256,956 - GFF525 0.60 +0.5 257,061 + GFF525 0.70 +0.1 257,062 - GFF525 0.70 -0.3 257,152 - GFF525 0.78 +2.7 257,290 + +0.1 257,360 + +0.6 257,469 - -0.0 257,493 - +0.7 257,493 - +1.9 257,679 + GFF526 0.11 -0.2 257,944 + GFF526 0.21 +0.0 257,944 + GFF526 0.21 +0.3 257,954 + GFF526 0.21 -0.4 258,000 + GFF526 0.23 +0.3 258,001 - GFF526 0.23 +0.4 258,002 + GFF526 0.23 -0.7 258,158 + GFF526 0.28 +0.2 258,180 + GFF526 0.29 -0.3
Or see this region's nucleotide sequence