Experiment: LB_plus_SM_buffer with 6H2_phage 0.5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF515 and GFF516 are separated by 210 nucleotides GFF516 and GFF517 are separated by 181 nucleotides
GFF515 - Protein involved in catabolism of external DNA, at 244,590 to 245,432
GFF515
GFF516 - Putative phosphatase, at 245,643 to 246,914
GFF516
GFF517 - Oligopeptidase A (EC 3.4.24.70), at 247,096 to 249,138
GFF517
Position (kb) 245
246
247 Strain fitness (log2 ratio) -2
-1
0
1 at 244.715 kb on + strand, within GFF515 at 244.821 kb on - strand, within GFF515 at 244.858 kb on + strand, within GFF515 at 244.880 kb on + strand, within GFF515 at 244.882 kb on + strand, within GFF515 at 245.192 kb on - strand, within GFF515 at 245.232 kb on - strand, within GFF515 at 245.252 kb on - strand, within GFF515 at 245.262 kb on - strand, within GFF515 at 245.381 kb on + strand at 245.382 kb on - strand at 245.442 kb on + strand at 245.455 kb on - strand at 245.471 kb on - strand at 245.488 kb on + strand at 245.534 kb on + strand at 245.642 kb on - strand at 245.657 kb on + strand at 245.662 kb on + strand at 245.708 kb on + strand at 245.709 kb on - strand at 245.819 kb on - strand, within GFF516 at 245.819 kb on - strand, within GFF516 at 245.819 kb on - strand, within GFF516 at 246.144 kb on + strand, within GFF516 at 246.144 kb on + strand, within GFF516 at 246.149 kb on + strand, within GFF516 at 246.182 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.257 kb on + strand, within GFF516 at 246.258 kb on - strand, within GFF516 at 246.258 kb on - strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.356 kb on + strand, within GFF516 at 246.455 kb on - strand, within GFF516 at 246.500 kb on + strand, within GFF516 at 246.501 kb on - strand, within GFF516 at 246.514 kb on - strand, within GFF516 at 246.519 kb on - strand, within GFF516 at 246.536 kb on + strand, within GFF516 at 246.537 kb on - strand, within GFF516 at 246.659 kb on + strand, within GFF516 at 246.659 kb on + strand, within GFF516 at 246.666 kb on + strand, within GFF516 at 246.684 kb on + strand, within GFF516 at 246.684 kb on + strand, within GFF516 at 246.684 kb on + strand, within GFF516 at 246.685 kb on - strand, within GFF516 at 246.956 kb on - strand at 246.956 kb on - strand at 246.968 kb on + strand at 246.992 kb on - strand at 247.112 kb on + strand at 247.113 kb on - strand at 247.113 kb on - strand at 247.206 kb on - strand at 247.206 kb on - strand at 247.237 kb on - strand at 247.237 kb on - strand at 247.290 kb on + strand at 247.351 kb on + strand, within GFF517 at 247.414 kb on + strand, within GFF517 at 247.599 kb on + strand, within GFF517 at 247.848 kb on + strand, within GFF517 at 247.849 kb on - strand, within GFF517
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6H2_phage 0.5 MOI remove 244,715 + GFF515 0.15 +0.0 244,821 - GFF515 0.27 +0.3 244,858 + GFF515 0.32 -0.3 244,880 + GFF515 0.34 +0.2 244,882 + GFF515 0.35 +0.1 245,192 - GFF515 0.71 -1.1 245,232 - GFF515 0.76 +0.9 245,252 - GFF515 0.79 -0.9 245,262 - GFF515 0.80 +0.3 245,381 + -0.0 245,382 - -0.1 245,442 + -0.1 245,455 - -0.2 245,471 - -0.6 245,488 + +0.1 245,534 + +0.3 245,642 - -1.4 245,657 + -0.4 245,662 + -0.3 245,708 + +0.3 245,709 - +0.1 245,819 - GFF516 0.14 +0.7 245,819 - GFF516 0.14 +0.6 245,819 - GFF516 0.14 -0.3 246,144 + GFF516 0.39 +0.4 246,144 + GFF516 0.39 +0.3 246,149 + GFF516 0.40 -0.1 246,182 + GFF516 0.42 -0.1 246,257 + GFF516 0.48 +0.1 246,257 + GFF516 0.48 +0.6 246,257 + GFF516 0.48 -0.2 246,257 + GFF516 0.48 -0.1 246,258 - GFF516 0.48 +0.2 246,258 - GFF516 0.48 +0.3 246,356 + GFF516 0.56 -0.4 246,356 + GFF516 0.56 -0.0 246,356 + GFF516 0.56 +0.0 246,455 - GFF516 0.64 -0.1 246,500 + GFF516 0.67 -0.2 246,501 - GFF516 0.67 +0.5 246,514 - GFF516 0.68 -0.6 246,519 - GFF516 0.69 -0.8 246,536 + GFF516 0.70 +0.2 246,537 - GFF516 0.70 -0.5 246,659 + GFF516 0.80 +0.8 246,659 + GFF516 0.80 +0.3 246,666 + GFF516 0.80 +0.3 246,684 + GFF516 0.82 +0.3 246,684 + GFF516 0.82 +0.4 246,684 + GFF516 0.82 +0.1 246,685 - GFF516 0.82 +0.8 246,956 - -0.3 246,956 - +0.8 246,968 + +0.2 246,992 - +0.3 247,112 + +0.9 247,113 - -0.3 247,113 - +0.3 247,206 - -0.2 247,206 - -0.9 247,237 - -1.2 247,237 - -0.2 247,290 + -0.7 247,351 + GFF517 0.12 -0.0 247,414 + GFF517 0.16 +0.4 247,599 + GFF517 0.25 -0.1 247,848 + GFF517 0.37 +0.2 247,849 - GFF517 0.37 -2.7
Or see this region's nucleotide sequence